FB2024_04 , released June 25, 2024
Gene: Dmel\cpo
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General Information
Symbol
Dmel\cpo
Species
D. melanogaster
Name
couch potato
Annotation Symbol
CG43738
Feature Type
FlyBase ID
FBgn0263995
Gene Model Status
Stock Availability
Gene Summary
couch potato (cpo) is most strongly expressed in nuclei of the central and peripheral nervous systems and the ring gland, where its product may regulate gene transcription to control complex neurological and neuroendocrine functions. It is required for normal synaptic transmission, climate adaptation, olfaction, diapause and behavioral responses. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-61
RefSeq locus
NT_033777 REGION:17919832..18018892
Sequence
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN002574557
enables RNA binding
inferred from electronic annotation with InterPro:IPR000504
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from mutant phenotype
involved_in dormancy process
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
couch potato (cpo) is most strongly expressed in nuclei of the central and peripheral nervous systems and the ring gland, where its product may regulate gene transcription to control complex neurological and neuroendocrine functions. It is required for normal synaptic transmission, climate adaptation, olfaction, diapause and behavioral responses. [Date last reviewed: 2019-03-07]
Protein Function (UniProtKB)
May play a role in the development or function of the peripheral nervous system by regulating the processing of nervous system-specific transcripts.
(UniProt, Q01617)
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
7

Please see the JBrowse view of Dmel\cpo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q01617)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.44

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.53

Multiphase exon postulated: exon reading frame differs in alternative transcripts.

Stop-codon suppression (UGA) postulated; FBrf0216885.

Unconventional translation start (CUG) postulated: conserved CUG (downstream of site proposed in GenBank accession Z14974, FBrf0056119).

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0330269
5675
606
FBtr0330270
9118
748
FBtr0330271
5461
841
FBtr0330272
4524
762
FBtr0330273
7247
749
FBtr0330274
9229
962
FBtr0330275
9743
607
FBtr0330276
5086
606
FBtr0330277
8397
607
FBtr0330278
4469
606
FBtr0330279
6116
606
FBtr0330280
6131
606
Additional Transcript Data and Comments
Reported size (kB)

7.8, 5.0, 4.5 (northern blot); 3.035 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0303301
63.3
606
9.45
FBpp0303305
77.5
749
8.70
FBpp0303306
100.4
962
9.07
FBpp0303307
63.4
607
9.45
FBpp0303308
63.3
606
9.45
FBpp0303309
63.4
607
9.45
FBpp0303310
63.3
606
9.45
FBpp0303311
63.3
606
9.45
FBpp0303312
63.3
606
9.45
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

606 aa isoforms: cpo-PN, cpo-PU, cpo-PW, cpo-PX, cpo-PY
607 aa isoforms: cpo-PT, cpo-PV
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cpo using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.75

Transcript Expression
Polypeptide Expression
Expression Deduced from Reporters
Reporter: P{GT1}cpoBG02810
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacW}cpol3
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacZcpo.P}
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacZ}cpol2
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacZ}cpol6
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lArB}cpo1
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lArB}cpo2
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{PZ}cpo06015
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\cpo in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 104 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of cpo
Transgenic constructs containing regulatory region of cpo
Aberrations (Deficiencies and Duplications) ( 23 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
7 of 14
Yes
Yes
1  
7 of 14
Yes
Yes
1  
1 of 14
No
No
1  
1 of 14
No
No
2  
1 of 14
No
No
3  
1 of 14
No
No
2  
1 of 14
No
No
1 of 14
No
No
1  
1 of 14
No
No
1  
1 of 14
No
No
2  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (8)
7 of 14
Yes
Yes
7 of 14
Yes
Yes
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
Mus musculus (laboratory mouse) (8)
7 of 14
Yes
Yes
7 of 14
Yes
Yes
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (11)
5 of 13
Yes
Yes
4 of 13
No
Yes
4 of 13
No
Yes
4 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Danio rerio (Zebrafish) (14)
7 of 14
Yes
Yes
7 of 14
Yes
Yes
5 of 14
No
Yes
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
7 of 14
Yes
Yes
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
7 of 12
Yes
Yes
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (17)
7 of 13
Yes
Yes
6 of 13
No
Yes
5 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
2 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
Yes
Schizosaccharomyces pombe (Fission yeast) (5)
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
Yes
1 of 12
Yes
No
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:cpo. Refer to their site for version information.
Paralogs
Paralogs (via DIOPT v9.1)
Drosophila melanogaster (Fruit fly) (2)
1 of 13
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Other Interaction Browsers

    Please see the Physical Interaction reports below for full details
    RNA-RNA
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-61
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    90D1-90E1
    Limits computationally determined from genome sequence between P{PZ}l(3)0788207882 and P{EP}cpoEP3679
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    90D-90D
    (determined by in situ hybridisation)
    90D1-90D2
    (determined by in situ hybridisation)
    90D-90E
    (determined by in situ hybridisation)
    90D1-90D4
    (determined by in situ hybridisation)
    65D4-65D5
    66D10--13 67E5--7 85C3--5 90D4--5 96C8--9 96C8--D1
    90D2-90E2
    (determined by in situ hybridisation)
    90D1-90E1
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (44)
    Genomic Clones (72)
    cDNA Clones (68)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

        Candidate gene for quantitative trait (QTL) locus determining bristle number.

        Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

        The cpo gene encodes a nuclear protein, and achaete, scute and daughterless gene function are required for proper expression of cpo in the PNS.

        Mutant cpo individuals exhibit an abnormal and hypoactive behaviour.

        Relationship to Other Genes
        Source for database merge of

        Source for merge of: cpo E(sda)N

        Source for merge of: cpo CG18434

        Source for merge of: cpo BcDNA:AT31405

        Source for merge of: cpo CG12349 CG18435

        Source for merge of: cpo CG42457

        Additional comments

        Annotations CG31243 and CG42457 merged as CG43738 in release 5.44 of the genome annotation. Merge supported by stop codon read-through analysis (FBrf0216884).

        Annotations CG12349, CG18434 and CG18435 merged as CG31243 in release 3 of the genome annotation.

        Source for merge of cpo BcDNA:AT31405 was a shared cDNA ( date:030728 ).

        Nomenclature History
        Source for database identify of
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (29)
        Reported As
        Symbol Synonym
        BcDNA:AT31405
        CG18434
        E(sda)N
        cg18435
        l(3)01432
        l(3)j4A1
        l(3)j4D4
        l(3)j7E2
        l(3)j9B3
        l(3)rJ553
        l(3)s2336
        Secondary FlyBase IDs
        • FBgn0000363
        • FBgn0011025
        • FBgn0011358
        • FBgn0011368
        • FBgn0011405
        • FBgn0011416
        • FBgn0011503
        • FBgn0038560
        • FBgn0038561
        • FBgn0038562
        • FBgn0051243
        • FBgn0063288
        • FBgn0066121
        • FBgn0259934
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 100 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        References (194)