FB2024_04 , released June 25, 2024
Gene: Dmel\moi
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General Information
Symbol
Dmel\moi
Species
D. melanogaster
Name
modigliani
Annotation Symbol
CG42350
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261019
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Summary
modigliani (moi) encodes a fast evolving, not evolutionary conserved, protein required for telomere maintenance. It is a member of terminin capping-complex that prevents telomere fusions. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

l(3)S096713

Key Links
Genomic Location
Cytogenetic map
90F11-90F11
Sequence location
Recombination map
3-62
RefSeq locus
NT_033777 REGION:18255530..18257677
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q95RV2
(Raffa et al., 2009)
contributes_to single-stranded telomeric DNA binding
inferred from direct assay
(Zhang et al., 2016)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in protection from non-homologous end joining at telomere
inferred from mutant phenotype
(Komonyi et al., 2009)
inferred from genetic interaction with FLYBASE:mei-41; FB:FBgn0004367
(Komonyi et al., 2009)
inferred from genetic interaction with FLYBASE:tefu; FB:FBgn0045035
(Komonyi et al., 2009)
involved_in telomere capping
inferred from mutant phenotype
(Raffa et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in telomere capping
inferred by curator from GO:0000782,GO:0043047
(Zhang et al., 2016)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in chromosome, telomeric region
inferred from direct assay
(Komonyi et al., 2009, Raffa et al., 2009)
located_in polytene chromosome interband
inferred from direct assay
(Komonyi et al., 2009)
part_of telomere cap complex
inferred from direct assay
(Zhang et al., 2016, Raffa et al., 2010)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Snapshot
    modigliani (moi) encodes a fast evolving, not evolutionary conserved, protein required for telomere maintenance. It is a member of terminin capping-complex that prevents telomere fusions. [Date last reviewed: 2019-03-14]
    Protein Function (UniProtKB)
    Part of the MTV complex that associates with the HipHop-HOAP complex to form the terminin telomere-capping complex involved in telomere maintenance and prevention of telomere fusion (PubMed:19240120, PubMed:27835648). Potentially functions downstream of mei-41/ATR (PubMed:19240120). As part of the MTV complex binds single stranded DNA in a sequence-independent manner, protecting it from degradation (PubMed:27835648).
    (UniProt, B7Z0L8)
    Summary (Interactive Fly)

    a novel protein enriched at telomeres that is required to prevent telomeric fusion - forms a protein complex that is functionally analogous to shelterin

    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\moi for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure
    Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry B7Z0L8)

    If you don't see a structure in the viewer, refresh your browser.
    Model Confidence:
    • Very high (pLDDT > 90)
    • Confident (90 > pLDDT > 70)
    • Low (70 > pLDDT > 50)
    • Very low (pLDDT < 50)

    AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

    Experimentally Determined Structures
    Crossreferences
    Comments on Gene Model

    Gene model reviewed during 5.52

    Multiphase exon postulated: this gene shares a region of coding sequence with an overlapping gene, but different reading frames are utilized in the overlapping coding region.

    Sequence Ontology: Class of Gene
    gene
    gene_with_dicistronic_mRNA
    (Raffa et al., 2009, FlyBase Genome Annotators, 2008)
    nuclear_gene
    (FlyBase Genome Annotators, 2008)
    protein_coding_gene
    (FlyBase Genome Annotators, 2008)
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    moi-RA
    FBtr0299938
    NM_001144601
    2089
    178
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    UniProt
    RefSeq ID
    GenBank
    moi-PA
    FBpp0289215
    20.2
    178
    9.27
    B7Z0L8
    NP_001138073
    ACL83531
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Probably homodimerizes (PubMed:15684427). Component of the MTV complex, composed of moi/modigliani, tea and ver/verrocchio (PubMed:27835648). Interacts with ver/verrochio and tea (via C-terminus); the interactions are direct and require fully intact moi/modigliani and ver/verrocchio (PubMed:20679394, PubMed:27835648). The MTV complex is recruited to telomeres by the HipHop-HOAP complex, consisting of HipHop, cav/HOAP and Su(var)205/HP1 to form the terminin telomere-capping complex (PubMed:19181850, PubMed:20679394, PubMed:27835648). Interacts with cav/HOAP and Su(var)205/HP1; the interactions are direct (PubMed:19181850). Probably interacts with peo (via N-terminus and UBC domain) (PubMed:26110638).

    (UniProt, B7Z0L8)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\moi using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Testis-specificity index

    The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

    NA

    Transcript Expression
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in chromosome, telomeric region
    inferred from direct assay
    (Komonyi et al., 2009, Raffa et al., 2009)
    located_in polytene chromosome interband
    inferred from direct assay
    (Komonyi et al., 2009)
    part_of telomere cap complex
    inferred from direct assay
    (Zhang et al., 2016, Raffa et al., 2010)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    JBrowse - Visual display of RNA-Seq signals

    View Dmel\moi in JBrowse
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 12 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 15 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of moi
    Transgenic constructs containing regulatory region of moi
    Aberrations (Deficiencies and Duplications) ( 4 )
    Inferred from experimentation ( 4 )
    Gene disrupted in
    Df(3R)d58
    (Komonyi et al., 2009)
    Df(3R)d192
    (Komonyi et al., 2009)
    Df(3R)P14
    (Raffa et al., 2009)
    Gene partially disrupted in
    Df(3R)d189
    (Komonyi et al., 2005)
    Inferred from location ( 0 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    lethal
    lethal (with moi1)
    lethal (with moiCB02140)
    lethal (with moiS096713)
    lethal | recessive
    lethal - all die during larval stage
    lethal - all die during P-stage
    lethal - all die during pupal stage, with Df(3R)d189/Df(3R)d58
    lethal - all die during pupal stage, with Df(3R)d189/Df(3R)d192
    lethal - all die during pupal stage (with Df(3R)d189)
    viable, with Df(3R)d189/moiS096713
    viable (with Df(3R)d189), with moium.t4.6
    Other Phenotypes
    Allele
    abnormal locomotor behavior, with Df(3R)d189/moiS096713
    abnormal locomotor behavior (with Df(3R)d189), with moium.t4.6
    abnormal mitotic cell cycle | larval stage
    short lived, with Df(3R)d189/moiS096713
    short lived (with Df(3R)d189), with moium.t4.6
    Phenotype manifest in
    Allele
    adult thoracic sensillum, with Df(3R)d189/moiS096713
    adult thoracic sensillum (with Df(3R)d189), with moium.t4.6
    chromosome
    chromosome, telomeric region
    chromosome, telomeric region, with Df(3R)d189/Df(3R)d58
    chromosome, telomeric region, with Df(3R)d189/Df(3R)d192
    chromosome, telomeric region, with Df(3R)d189/moiS096713
    chromosome, telomeric region (with Df(3R)d189), with moium.t4.6
    chromosome, telomeric region (with Df(3R)P14)
    chromosome, telomeric region (with moi1)
    chromosome, telomeric region (with moiCB02140)
    chromosome, telomeric region (with moiS096713)
    chromosome | larval stage
    eye, with Df(3R)d189/moiS096713
    eye (with Df(3R)d189), with moium.t4.6
    larval neuroblast
    wing margin, with Df(3R)d189/moiS096713
    wing margin (with Df(3R)d189), with moium.t4.6
    wing vein, with Df(3R)d189/moiS096713
    wing vein (with Df(3R)d189), with moium.t4.6
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (0)
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (0)
    Mus musculus (laboratory mouse) (0)
    Xenopus tropicalis (Western clawed frog) (0)
    Danio rerio (Zebrafish) (0)
    Caenorhabditis elegans (Nematode, roundworm) (0)
    Anopheles gambiae (African malaria mosquito) (1)
    Agam\AgaP_AGAP004911
    2 of 12
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    Schizosaccharomyces pombe (Fission yeast) (0)
    Escherichia coli (enterobacterium) (0)
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:moi. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      esyN Network Key:
      Suppression
      Enhancement
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      moi
      suppressible
      mei-41
      (Komonyi et al., 2009)
      moi
      enhanceable
      tefu
      (Komonyi et al., 2009)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      cav
      suppressible
      moi
      (Dubruille and Loppin, 2015)
      tefu
      enhanceable
      moi
      (Komonyi et al., 2009)
      Tgs1
      suppressible
      moi
      (Cheng et al., 2020)
      External Data
      Subunit Structure (UniProtKB)
      Probably homodimerizes (PubMed:15684427). Component of the MTV complex, composed of moi/modigliani, tea and ver/verrocchio (PubMed:27835648). Interacts with ver/verrochio and tea (via C-terminus); the interactions are direct and require fully intact moi/modigliani and ver/verrocchio (PubMed:20679394, PubMed:27835648). The MTV complex is recruited to telomeres by the HipHop-HOAP complex, consisting of HipHop, cav/HOAP and Su(var)205/HP1 to form the terminin telomere-capping complex (PubMed:19181850, PubMed:20679394, PubMed:27835648). Interacts with cav/HOAP and Su(var)205/HP1; the interactions are direct (PubMed:19181850). Probably interacts with peo (via N-terminus and UBC domain) (PubMed:26110638).
      (UniProt, B7Z0L8 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-62
      Cytogenetic map
      90F11-90F11
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      90F11-90F11
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-62

      (FlyBase, 2016)

      3-61.9

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (4)
      Genomic Clones (22)
      cDNA Clones (14)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Mutant larvae have increased frequencies of telomeric fusions in brain cells.

            (Cenci et al., 1999)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: moi tlu

            (Raffa et al., 2009)
            Additional comments

            One or more of the processed transcripts for these genes contain two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG42350 (FBgn0259681), CG31241 (FBgn0051241).

            (FlyBase Genome Annotators, 2008)

            One or more of the processed transcripts for this gene encodes two overlapping ORFs in different reading frames, represented by tatl (downstream ORF) and moi (upstream ORF).

            (Komonyi et al., 2005)
            Nomenclature History
            Source for database identify of

            Source for identity of: tlu CG42350

            (Komonyi et al., 2005)
            Nomenclature comments

            FlyBase curator comment: In 2005, Komonyi et al. (FBrf0184045) referred to the original single CG31241 annotation, which produces a dicistronic transcript, as 'dtl'. In February 2009, Raffa et al. (FBrf0206682) characterized the upstream ORF, referring to it as 'moi' (which now has its own annotation ID of CG42350), and retained the 'dtl' designation for the downstream ORF (which now has its own annotation ID of CG44890). In March 2009, Komonyi et al. (FBrf0207599) also characterized the upstream ORF (CG42350) but retained the 'dtl'/'DTL' designation for it, while referring to the downstream ORF (CG44890) as 'TGS1'. In an attempt to reduce confusion, the upstream ORF (CG42350) is named 'moi' in FlyBase (according to FBrf0206682) and the downstream ORF (CG44890) is named 'Tsg1' in FlyBase (according to FBrf0207599), with 'dtl' being retained as a synonym of both.

            (Komonyi et al., 2009)
            Etymology

            The gene is named "modigliani" after the name of an Italian train, after the mutant phenotype of multiple telomeric associations in the same metaphase spread, which often results in multicentric linear chromosomes that resemble little "trains" of chromosomes.

            (Raffa et al., 2009)

            Named "modigliani" after an Italian train, as the multicentric chromosomes observed in mutants resemble little trains of chromosomes.

            (Cenci et al., 1999)
            Synonyms and Secondary IDs (18)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 20 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (41)