FB2024_02 , released April 23, 2024
Gene: Dmel\Pop2
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General Information
Symbol
Dmel\Pop2
Species
D. melanogaster
Name
Pop2
Annotation Symbol
CG5684
Feature Type
protein_coding_gene
FlyBase ID
FBgn0036239
Gene Model Status
Current
Stock Availability
13 publicly available
Enzyme Name (EC)
poly(A)-specific ribonuclease (3.1.13.4)
Gene Summary
Pop2 (Pop2) encodes a poly(A)-specific ribonuclease involved in translation inhibition. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)
All Summaries
Also Known As

CAF1

Key Links
Genomic Location
Cytogenetic map
68F1-68F1
Sequence location
Recombination map
3-38
RefSeq locus
NT_037436 REGION:11998398..12002915
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables poly(A)-specific ribonuclease activity
inferred from direct assay
(Temme et al., 2004)
inferred from mutant phenotype
(Temme et al., 2010)
enables protein binding
inferred from physical interaction with UniProtKB:Q7KQM6
(Ruscica et al., 2019)
inferred from physical interaction with UniProtKB:Q8SY33
(Braun et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR036397
(InterPro Project Members, 2004-)
enables poly(A)-specific ribonuclease activity
inferred from electronic annotation with InterPro:IPR039637
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000084083
(GO Reference Genome Project, 2011-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in mRNA catabolic process
inferred from direct assay
(Bawankar et al., 2013)
involved_in negative regulation of translation
inferred from direct assay
(Bawankar et al., 2013)
involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
inferred from mutant phenotype
(Arvola et al., 2020)
involved_in nuclear-transcribed mRNA poly(A) tail shortening
inferred from mutant phenotype
(Temme et al., 2010, Bönisch et al., 2007)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of CCR4-NOT complex
inferred from direct assay
(Temme et al., 2010, Temme et al., 2004)
inferred from physical interaction with FLYBASE:Marf1; FB:FBgn0039972
(Zhu et al., 2018)
located_in cytoplasm
inferred from direct assay
(Temme et al., 2004)
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of CCR4-NOT complex
inferred from electronic annotation with InterPro:IPR039637
(InterPro Project Members, 2004-)
part_of CCR4-NOT core complex
inferred from biological aspect of ancestor with PANTHER:PTN000084083
(GO Reference Genome Project, 2011-)
is_active_in P-body
inferred from biological aspect of ancestor with PANTHER:PTN000084083
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
poly(A)-specific ribonuclease activity
Exonucleolytic cleavage of poly(A) to 5'-AMP (3.1.13.4)
Summaries
Gene Snapshot
Pop2 (Pop2) encodes a poly(A)-specific ribonuclease involved in translation inhibition. [Date last reviewed: 2019-08-01]
Gene Group (FlyBase)
CCR4-NOT COMPLEX -
The CCR4-NOT complex is a conserved multi-subunit complex implicated in various cellular processes to regulated gene expression. It is best characterised in its role as the main enzyme responsible for the deadenylation of mRNA. The conserved core of the complex is composed of two modules: the NOT module and a catalytic module comprising two deadenylases, twin and Pop2. (Adapted from FBrf0221179 and FBrf0231273).
3'-5' RNA EXONUCLEASES -
3'-5'-exoribonucleases catalyze the sequential degradation of mononucleotides from a free 3' terminus of an RNA molecule.
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
5

Please see the JBrowse view of Dmel\Pop2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry X2JCC3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Stop-codon suppression (UGA) postulated; FBrf0243886.

Gene model reviewed during 6.33

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Pop2-RA
FBtr0076058
NM_140281
1688
297
Pop2-RB
FBtr0076059
NM_168484
1893
293
Pop2-RC
FBtr0076060
NM_168485
1827
293
Pop2-RD
FBtr0300272
NM_001169958
1531
357
Pop2-RE
FBtr0344758
NM_001300112
1750
361
Pop2-RG
FBtr0479823
NM_001382181
1688
341
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Pop2-PA
FBpp0075790
34.0
297
4.85
Q9VTS4
NP_648538
AAF49972
Pop2-PB
FBpp0075791
33.5
293
4.77
Q8I0I5
NP_729775
AAN12248
Pop2-PC
FBpp0075792
33.5
293
4.77
Q8I0I5
NP_729776
AAN12249
Pop2-PD
FBpp0289500
40.2
357
5.35
E1JHY7
NP_001163429
ACZ94700
Pop2-PE
FBpp0311089
40.7
361
5.52
X2JCC3
NP_001287041
AHN58066
Pop2-PG
FBpp0428156
38.7
341
4.81
QJC18617
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

293 aa isoforms: Pop2-PB, Pop2-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pop2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.93

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
hindgut anlage
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
anterior endoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
procephalic neuroblast
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
ovary
(Temme et al., 2004)
oogenesis stage S10
follicle cell
(Temme et al., 2004)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- larval stage
(Temme et al., 2004)
adult stage | female
ovary
(Temme et al., 2004)
adult stage | male
organism
(Temme et al., 2004)
Additional Descriptive Data

Expression is observed in all cells of the ovary at all stages of oogenesis.

(Temme et al., 2004)
Marker for
 
ovary
(Temme et al., 2004)
Subcellular Localization
CV Term
Evidence
References
part_of CCR4-NOT complex
inferred from direct assay
(Temme et al., 2010, Temme et al., 2004)
inferred from physical interaction with FLYBASE:Marf1; FB:FBgn0039972
(Zhu et al., 2018)
located_in cytoplasm
inferred from direct assay
(Temme et al., 2004)
located_in cytosol
inferred from high throughput direct assay
(Lye et al., 2014)
Expression Deduced from Reporters
Reporter: PBac{754.P.FSVS-0}Pop2CPTI002818
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pop2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pop2
Transgenic constructs containing regulatory region of Pop2
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3L)Exel6115
(Ryder, 2004.4.26)
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
Hsap\CNOT7
14 of 14
Yes
Yes
1  
Hsap\CNOT8
13 of 14
No
Yes
Hsap\PARN
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
Rnor\Cnot7
14 of 14
Yes
Yes
Rnor\Cnot8
12 of 14
No
Yes
Rnor\Parn
1 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Cnot7
13 of 14
Yes
Yes
Mmus\Cnot8
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\cnot7
11 of 13
Yes
Yes
Xtro\cnot8
6 of 13
No
Yes
Xtro\toe1
1 of 13
No
Yes
Xtro\ube2v2
1 of 13
No
No
Danio rerio (Zebrafish) (3)
Drer\cnot7
14 of 14
Yes
Yes
Drer\cnot8
13 of 14
No
Yes
Drer\toe1
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\ccf-1
14 of 14
Yes
Yes
Cele\parn-1
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP011413
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (11)
Atha\AT5G10960
13 of 13
Yes
Yes
Atha\AT1G80780
12 of 13
No
Yes
Atha\AT2G32070
12 of 13
No
Yes
Atha\AT1G15920
11 of 13
No
Yes
Atha\CAF1a
7 of 13
No
Yes
Atha\CAF1b
7 of 13
No
Yes
Atha\AT1G06450
4 of 13
No
Yes
Atha\AT1G27820
4 of 13
No
Yes
Atha\AT1G27890
4 of 13
No
Yes
Atha\AT1G61470
4 of 13
No
Yes
Atha\AT3G44240
4 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\POP2
11 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\caf1
12 of 12
Yes
Yes
Spom\SPBC29A10.09c
1 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Pop2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Pop2HM05235
model of  heart disease
CEA
(Elmén et al., 2020)
Pop2GD12804
model of  cancer
CEA with DeltaUAS.cDa
(Vicente et al., 2018)
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Pop2DG14804
ameliorates  oculopharyngeal muscular dystrophy
modeled by Hsap\PABPN117ala.Act88F
(Chartier et al., 2015)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
CNOT7; CCR4-NOT transcription complex subunit 7
14 of 14
604913
       
      CNOT8; CCR4-NOT transcription complex subunit 8
      13 of 14
      603731
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Show neighbor-neighbor interactions:
          Show/hide secondary interactors 
          (data from AllianceMine provided by esyN)
          Select Layout:
          Legend:
          Protein
          RNA
          Selected Interactor(s)
          Interactions Browser
          View in Interactions Browser
          Please see the Physical Interaction reports below for full details
          MIST Molecular Interaction Search Tool
          View in MIST tool (external link)
          Please see the Physical Interaction reports below for full details
          protein-protein
          Physical Interaction
          Assay
          References
          Pop2 - AGO1
          anti bait coimmunoprecipitation, western blot
          (Iwasaki et al., 2009)
          Pop2 - Gyf
          anti tag coimmunoprecipitation, anti tag western blot
          (Ruscica et al., 2019)
          Pop2 - Not1
          anti tag coimmunoprecipitation, anti tag western blot, molecular sieving, molecular weight estimation by staining, anti bait coimmunoprecipitation, Identification by mass spectrometry, experimental knowledge based, western blot
          (Pekovic and Wahle, 2024, Bawankar et al., 2013, Petit et al., 2012, Guruharsha et al., 2011, Temme et al., 2010)
          Pop2 - Not3
          molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot
          (Pekovic and Wahle, 2024, Zekri et al., 2009)
          Pop2 - Patr-1
          anti tag coimmunoprecipitation, anti tag western blot
          (Haas et al., 2010)
          Pop2 - Rcd-1
          molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot
          (Pekovic and Wahle, 2024, Bawankar et al., 2013)
          Pop2 - Rga
          molecular sieving, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot
          (Pekovic and Wahle, 2024, Haugen et al., 2022)
          Pop2 - Tob
          anti tag coimmunoprecipitation, anti tag western blot, two hybrid
          (Hu et al., 2017.6.13, White-Grindley et al., 2014)
          Pop2 - cu
          anti tag coimmunoprecipitation, western blot
          (Temme et al., 2010)
          Pop2 - cup
          anti tag coimmunoprecipitation, anti tag western blot
          (Igreja and Izaurralde, 2011)
          Pop2 - pum
          pull down, anti tag western blot, autoradiography
          (Weidmann et al., 2014, Kadyrova et al., 2007)
          Pop2 - roq
          anti tag coimmunoprecipitation, anti tag western blot
          (Sgromo et al., 2017)
          Pop2 - smg
          anti tag coimmunoprecipitation, western blot
          (Semotok et al., 2005)
          Pop2 - twin
          ion exchange chromatography, molecular sieving, western blot, anti tag coimmunoprecipitation, experimental knowledge based, molecular weight estimation by staining, anti tag western blot
          (Pekovic and Wahle, 2024, Fu et al., 2015, Bawankar et al., 2013, Joly et al., 2013, Petit et al., 2012, Guruharsha et al., 2011, Temme et al., 2010, Temme et al., 2004)
          RNA-protein
          Physical Interaction
          Assay
          References
          Pop2 - nanos
          pull down, western blot, Identification by mass spectrometry
          (Götze et al., 2017, Jeske et al., 2011)
          Summary of Genetic Interactions
          esyN Network Diagram
          Show/hide secondary interactors 
          (data from AllianceMine provided by esyN)
          esyN Network Key:
          Suppression
          Enhancement
          View in Interactions Browser   View in MIST tool (external link)
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Pop2
          suppressible
          Tis11
          (McClelland et al., 2017)
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          brat
          enhanceable
          Pop2
          (Komori et al., 2018)
          Delta
          enhanceable
          Pop2
          (Vicente et al., 2018)
          Pink1
          suppressible
          Pop2
          (Gehrke et al., 2015)
          Tis11
          suppressible
          Pop2
          (McClelland et al., 2017)
          External Data
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          External Data
          Linkouts
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3L
          Recombination map
          3-38
          Cytogenetic map
          68F1-68F1
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          68F1-68F1
          Limits computationally determined from genome sequence between P{PZ}CycA02461&P{PZ}CycA03946 and P{PZ}rols08232&P{lacW}l(3)j2D3j2D3
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          3-38

          (FlyBase, 2016)

          3-30.4

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (13)
          Genomic Clones (24)
          cDNA Clones (141)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           

          polyclonal

          (Temme et al., 2004)
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
              Other Comments

              dsRNA made from templates generated with primers directed against this gene are used to study its function.

              (Behm-Ansmant et al., 2006)

              dsRNA has been made from templates generated with primers directed against this gene. RNAi of Pop2 causes dorsal overextension of the primary dendrite in ddaE and an overall increase in dendritic length in both ddaD and ddaE neurons. RNAi also causes defects in muscle and defects in dendrite morphogenesis.

              (Parrish et al., 2006)

              Depletion of Pop2 using dsRNA interference (RNAi) in S2 cells results in a strong increase in the average mRNA poly(A) length.

              (Temme et al., 2004)
              Relationship to Other Genes
              Source for database merge of
              Additional comments
              Nomenclature History
              Source for database identify of

              Source for identity of: CG5684 Pop2

              (Semotok et al., 2005)
              Nomenclature comments
              Etymology
              Synonyms and Secondary IDs (7)
              Reported As
              Symbol Synonym
              CAF1
              (Niinuma and Tomari, 2017, Sgromo et al., 2017, Joly et al., 2013, Collart and Panasenko, 2012, Jäger and Dorner, 2010, Temme et al., 2010, Lauwers et al., 2009, Bönisch et al., 2007, Behm-Ansmant et al., 2006, Bjorklund et al., 2006, Siomi et al., 2005, Temme et al., 2004)
              CG5684
              (Kolkhof et al., 2017, Meadows, 2017.1.11, Shan et al., 2017, Schertel et al., 2013, Jäger and Dorner, 2010, Ni et al., 2010.12.1, Perkins et al., 2009, Bjorklund et al., 2006, Parrish et al., 2006, Myrick et al., 2005.5.16, Brody et al., 2002)
              CNOT7
              (Sgromo et al., 2017)
              Caf1
              (Pekovic and Wahle, 2024, Zhu et al., 2018, Götze et al., 2017, Jeske et al., 2011, Temme et al., 2010, Iwasaki et al., 2009)
              POP2
              (Foley et al., 2019, Ruscica et al., 2019, Shimaji et al., 2016, Temme et al., 2014, Bawankar et al., 2013, Nishihara et al., 2013, Zekri et al., 2013, Petit et al., 2012, Kondo and Perrimon, 2011, Haas et al., 2010, Neely et al., 2010, Temme et al., 2010, Zekri et al., 2009, Seydoux and Braun, 2006, Semotok et al., 2005)
              Pop2
              (Titlow et al., 2023, Haugen et al., 2022, Kamiyama and Niwa, 2022, Arvola et al., 2020, Elmén et al., 2020, FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-, Grima et al., 2019, Jia et al., 2019, Lai et al., 2019, Story et al., 2019, Vicente et al., 2018, Zeng et al., 2018, Hu et al., 2017.6.13, McClelland et al., 2017, Shan et al., 2017, Vrettos et al., 2017, Brewer-Jensen et al., 2016, Gene Disruption Project members, 2016-, Chartier et al., 2015, Fu et al., 2015, Gehrke et al., 2015, Newton et al., 2015, Okada et al., 2015, Temme et al., 2014, Weidmann et al., 2014, White-Grindley et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Jäger and Dorner, 2010, Temme et al., 2010, Iwasaki et al., 2009, Kadyrova et al., 2007)
              pop2
              (Flora et al., 2018, Komori et al., 2018, Lauwers et al., 2009)
              Name Synonyms
              Pop2
              Secondary FlyBase IDs
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 67 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                References (110)