FB2024_04 , released June 25, 2024
Gene: Dmel\mof
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General Information
Symbol
Dmel\mof
Species
D. melanogaster
Name
males absent on the first
Annotation Symbol
CG3025
Feature Type
protein_coding_gene
FlyBase ID
FBgn0014340
Gene Model Status
Current
Stock Availability
11 publicly available
Enzyme Name (EC)
histone acetyltransferase (2.3.1.48)
Gene Summary
males absent on the first (mof) encodes a MYST family histone acetyltransferase that acetylates the product of His4 at Lysine 16. It is part of the male-specific lethal complex that mediates dosage compensation by transcriptional upregulation of the male X chromosome. In addition, it is part of the non-specific lethal complex which regulates expression of housekeeping genes in males and females. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

max, males absent on first, dMOF, males-absent on the X

Key Links
Genomic Location
Cytogenetic map
5C5-5C5
Sequence location
Recombination map
1-14
RefSeq locus
NC_004354 REGION:5873840..5876853
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (24 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Straub et al., 2008)
enables histone acetyltransferase activity
inferred from direct assay
(Schiemann et al., 2010)
enables histone H4K16 acetyltransferase activity
inferred from direct assay
(Samata et al., 2020, Prestel et al., 2010, Kind et al., 2008, Akhtar and Becker, 2000)
inferred from mutant phenotype
(Bhadra et al., 2012, Conrad et al., 2012, Schiemann et al., 2010)
enables lncRNA binding
inferred from direct assay
(Akhtar et al., 2000)
enables protein binding
inferred from physical interaction with UniProtKB:A1Z8P9
(Aleman et al., 2021)
inferred from physical interaction with FLYBASE:nsl1; FB:FBgn0262527
(Raja et al., 2010)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from biological aspect of ancestor with PANTHER:PTN004172926
(GO Reference Genome Project, 2011-)
enables histone acetyltransferase activity
inferred from sequence or structural similarity with SGD:S000000148
(Hilfiker et al., 1997)
inferred from sequence or structural similarity with SGD:S000004734
(Hilfiker et al., 1997)
traceable author statement
(Carrozza et al., 2003)
inferred from electronic annotation with InterPro:IPR002717
(InterPro Project Members, 2004-)
enables histone H4K16 acetyltransferase activity
traceable author statement
(Brehm et al., 2004)
inferred from biological aspect of ancestor with PANTHER:PTN007449682
(GO Reference Genome Project, 2011-)
enables transcription coregulator activity
inferred from biological aspect of ancestor with PANTHER:PTN004172926
(GO Reference Genome Project, 2011-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in DNA damage response, signal transduction by p53 class mediator
inferred from mutant phenotype
(Bhadra et al., 2012)
involved_in DNA repair-dependent chromatin remodeling
inferred from mutant phenotype
(Bhadra et al., 2012)
involved_in dosage compensation by hyperactivation of X chromosome
inferred from direct assay
(Samata et al., 2020, Kind et al., 2008)
involved_in positive regulation of DNA-templated transcription
inferred from mutant phenotype
(Schiemann et al., 2010)
involved_in positive regulation of gene expression, epigenetic
inferred from direct assay
(Samata et al., 2020)
involved_in sex-chromosome dosage compensation
inferred from direct assay
(Conrad et al., 2012)
inferred from mutant phenotype
(Hilfiker et al., 1997)
inferred from genetic interaction with FLYBASE:msl-2; FB:FBgn0005616
(Hilfiker et al., 1997)
inferred from mutant phenotype
(Aleman et al., 2021)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in dosage compensation by hyperactivation of X chromosome
non-traceable author statement
(Schutt and Nothiger, 2000)
involved_in regulation of DNA-templated transcription
inferred from electronic annotation with InterPro:IPR002717
(InterPro Project Members, 2004-)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN004172926
(GO Reference Genome Project, 2011-)
Cellular Component (10 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
part_of MSL complex
inferred from direct assay
(Meller et al., 2000)
inferred from direct assay
(Hallacli et al., 2012, Schiemann et al., 2010)
part_of NSL complex
inferred from direct assay
(Lam et al., 2012, Prestel et al., 2010)
inferred from direct assay
(Raja et al., 2010)
located_in nuclear chromosome
inferred from direct assay
(Figueiredo et al., 2014)
located_in nucleus
inferred from direct assay
(Raja et al., 2010)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
part_of transcription regulator complex
inferred from direct assay
(Prestel et al., 2010)
part_of X chromosome located dosage compensation complex, transcription activating
inferred from direct assay
(Conrad et al., 2012, Kind et al., 2008, Akhtar et al., 2000, Meller et al., 2000)
inferred from direct assay
(Prestel et al., 2010)
is_active_in X chromosome
inferred from direct assay
(Conrad et al., 2012, Kind et al., 2008)
located_in X chromosome
inferred from direct assay
(Akhtar et al., 2000)
inferred from direct assay
(Schunter et al., 2017)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
part_of chromatin
inferred from biological aspect of ancestor with PANTHER:PTN004172926
(GO Reference Genome Project, 2011-)
part_of MSL complex
inferred from biological aspect of ancestor with PANTHER:PTN008308198
(GO Reference Genome Project, 2011-)
part_of NSL complex
inferred from biological aspect of ancestor with PANTHER:PTN008308198
(GO Reference Genome Project, 2011-)
part_of NuA4 histone acetyltransferase complex
inferred from biological aspect of ancestor with PANTHER:PTN007449682
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN004172926
(GO Reference Genome Project, 2011-)
part_of X chromosome located dosage compensation complex, transcription activating
non-traceable author statement
(Schutt and Nothiger, 2000)
located_in X chromosome
traceable author statement
(Brehm et al., 2004)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MYST (SAS/MOZ) family. (O02193)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
histone acetyltransferase activity
acetyl-CoA + L-lysyl-[protein] = CoA + H(+) + N(6)-acetyl-L-lysyl- [protein] (2.3.1.48)
RHEA 21992: histone H4K16 acetyltransferase activity
acetyl-CoA + L-lysyl-[protein] = CoA + H(+) + N(6)-acetyl-L-lysyl- [protein] (2.3.1.48)
Summaries
Gene Snapshot
males absent on the first (mof) encodes a MYST family histone acetyltransferase that acetylates the product of His4 at Lysine 16. It is part of the male-specific lethal complex that mediates dosage compensation by transcriptional upregulation of the male X chromosome. In addition, it is part of the non-specific lethal complex which regulates expression of housekeeping genes in males and females. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
MALE SPECIFIC LETHAL COMPLEX -
The Male Specific Lethal (MSL) complex is a chromatin modifying complex composed of five protein subunits and two non-coding RNAs. MSL is involved in X chromosome dosage compensation in males. (Adapted from FBrf0228243).
HISTONE ACETYLTRANSFERASES -
Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).
Protein Function (UniProtKB)
Histone acetyltransferase that catalyzes the formation of the majority of histone H4 acetylation at 'Lys-16' (H4K16ac), an epigenetic mark that prevents chromatin compaction and constitutes the only acetylation mark intergenerationally transmitted (PubMed:10882077, PubMed:18510926, PubMed:20620953, PubMed:22421046, PubMed:32502394). Catalytic component of the male-specific lethal (MSL) complex, a multiprotein complex essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation) (PubMed:10882077, PubMed:11014199, PubMed:16543150, PubMed:18510926, PubMed:34133927, PubMed:9155031). The MSL complex specifically associates with the single X chromosome in males and mediates formation of H4K16ac, promoting a two-fold activation of X chromosome (PubMed:11258702, PubMed:18510926, PubMed:20620953, PubMed:22421046). Dosage compensation ensures that males with a single X chromosome have the same amount of most X-linked gene products as females with two X chromosomes (PubMed:18510926, PubMed:9155031). In oocytes, mof is also part of a maternal MSL subcomplex that mediates H4K16ac deposition for intergenerational transmission: H4K16ac prepares the chromatin landscape for establishment of nucleosome accessibility and poises genes for future activation (PubMed:32502394). H4K16ac constitutes the only acetylation mark maintained from oocytes to fertilized embryos (PubMed:32502394). Mof also constitutes the catalytic component of the non-specific lethal (NLS) complex, which promotes expression of housekeeping genes on X chromosome and autosomes (PubMed:20620953, PubMed:20620954, PubMed:22723752). The NSL complex promotes strong expression of housekeeping genes compared to the two-fold expression mediated by the MSL complex on X chromosome, suggesting that the activation potential of mof is constrained in the context of dosage compensation (PubMed:20620953).
(UniProt, O02193)
Summary (Interactive Fly)

a MYST family histone acetyltransferase that acetylates His4 at Lysine 16. Mof is part of the male-specific lethal complex that mediates dosage compensation by transcriptional upregulation of the male X chromosome - part of the non-specific lethal complex which regulates expression of housekeeping genes in males and females - regulates DIAP1 and induces apoptosis in a JNK dependent pathway

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mof for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O02193)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mof-RA
FBtr0070829
NM_078496
3014
827
Additional Transcript Data and Comments
Reported size (kB)

3.2 (northern blot)

(Hilfiker et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mof-PA
FBpp0070794
92.7
827
4.42
O02193
NP_511051
AAF46095
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

827 (aa)

(Hilfiker et al., 1997)
Comments
External Data
Post Translational Modification

Autoacetylation at Lys-638 is required for binding histone H4 with high affinity and for proper function.

Ubiquitinated by msl-2.

(UniProt, O02193)
Subunit Structure (UniProtKB)

Component of the male-specific lethal (MSL) histone acetyltransferase complex, composed of mof, mle, msl-1, msl-2 and msl-3 proteins, as well as roX1 and roX2 non-coding RNAs (PubMed:10679323, PubMed:11014199, PubMed:16543150, PubMed:18510926, PubMed:20620953, PubMed:20620954, PubMed:22421046). Component of a maternal MSL subcomplex composed of mof, msl-1 and msl-3 (PubMed:32502394). Component of the non-specific lethal (NLS) histone acetyltransferase complex at least composed of mof, nls1, dgt1/NSL2, Rcd1/NSL3, Rcd5/MCRS2, MBD-R2 and wds (PubMed:16543150, PubMed:20620953, PubMed:20620954, PubMed:22723752). In males, interacts with nucleoporin Mtor (PubMed:34133927).

(UniProt, O02193)
Domain

The tudor-knot domain (also named chromobarrel domain) mediates association with roX1 and roX2 non-coding RNAs, promoting recruitment to the male X chromosome (PubMed:11014199). It also potentiates the histone acetyltransferase activity after chromatin binding (PubMed:22421046).

(UniProt, O02193)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mof using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.21

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage -- adult stage
(Hilfiker et al., 1997)
Additional Descriptive Data

mof transcript is found in larvae and adults of both sexes.

(Hilfiker et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of MSL complex
inferred from direct assay
(Meller et al., 2000)
inferred from direct assay
(Hallacli et al., 2012, Schiemann et al., 2010)
part_of NSL complex
inferred from direct assay
(Lam et al., 2012, Prestel et al., 2010)
inferred from direct assay
(Raja et al., 2010)
located_in nuclear chromosome
inferred from direct assay
(Figueiredo et al., 2014)
located_in nucleus
inferred from direct assay
(Raja et al., 2010)
located_in polytene chromosome interband
inferred from direct assay
(Raja et al., 2010)
part_of transcription regulator complex
inferred from direct assay
(Prestel et al., 2010)
part_of X chromosome located dosage compensation complex, transcription activating
inferred from direct assay
(Conrad et al., 2012, Kind et al., 2008, Akhtar et al., 2000, Meller et al., 2000)
inferred from direct assay
(Prestel et al., 2010)
is_active_in X chromosome
inferred from direct assay
(Conrad et al., 2012, Kind et al., 2008)
located_in X chromosome
inferred from direct assay
(Akhtar et al., 2000)
inferred from direct assay
(Schunter et al., 2017)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mof in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 53 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mof
Transgenic constructs containing regulatory region of mof
Aberrations (Deficiencies and Duplications) ( 1 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal | male
lethal | male | recessive
lethal | male limited, with Scer\GAL4Act5C.PI
lethal - all die before end of larval stage | germline clone | maternal effect
lethal - all die before end of larval stage | male limited
lethal - all die during embryonic stage | germline clone | male limited | maternal effect
lethal - all die during larval stage, with Scer\GAL4e22c
partially lethal - majority die | female
partially lethal - majority die | female limited | germline clone
partially lethal - majority live | female limited
viable | female
viable | female limited (with mof3)
viable | female limited (with mof6)
Sterility
Allele
female fertile
female fertile, with Scer\GAL4nanos.PG
female sterile
Other Phenotypes
Allele
abnormal cell cycle | oogenesis, with Scer\GAL4nanos.PG
abnormal stress response | oogenesis, with Scer\GAL4nanos.PG
decreased cell growth rate, with Scer\GAL4ppl.PP
increased body size | female limited, with Scer\GAL4Act5C.PI
increased cell death | third instar larval stage
non-suppressor of variegation, with P{UAS-lacZ.Abd-B.5F24}25,2a
radiation sensitive | embryonic stage | male limited
radiation sensitive | first instar larval stage | male limited (with mof3)
radiation sensitive | first instar larval stage | male limited (with mof6)
radiation sensitive | larval stage
short lived | female
short lived | female limited | germline clone
wild-type
Phenotype manifest in
Allele
embryo | germline clone | male limited | maternal effect
embryonic/larval fat body, with Scer\GAL4ppl.PP
eye disc | third instar larval stage
female germline stem cell, with Scer\GAL4nanos.PG
wing disc | larval stage
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\KAT8
13 of 14
Yes
Yes
Hsap\KAT5
5 of 14
No
No
Hsap\KAT6A
3 of 14
No
No
Hsap\KAT6B
3 of 14
No
No
Hsap\KAT7
3 of 14
No
No
Hsap\SAMD1
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Kat8
13 of 14
Yes
Yes
Rnor\Kat5
4 of 14
No
No
Rnor\Kat6a
2 of 14
No
No
Rnor\Kat6b
2 of 14
No
No
Rnor\Kat7
2 of 14
No
No
Mus musculus (laboratory mouse) (6)
Mmus\Kat8
12 of 14
Yes
Yes
Mmus\Kat5
5 of 14
No
No
Mmus\Kat6a
3 of 14
No
No
Mmus\Kat6b
3 of 14
No
No
Mmus\Kat7
3 of 14
No
No
Mmus\Samd1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (11)
Xtro\kat8
7 of 13
Yes
Yes
Xtro\fmnl2
6 of 13
No
No
Xtro\ribc2
5 of 13
No
No
Xtro\kat5
2 of 13
No
No
Xtro\pde10a
2 of 13
No
No
Xtro\trim69.2
2 of 13
No
Yes
Xtro\appl1
1 of 13
No
Yes
Xtro\kat6a
1 of 13
No
No
Xtro\kat7
1 of 13
No
No
Xtro\trim69.1
1 of 13
No
No
Xtro\xnf7
1 of 13
No
No
Danio rerio (Zebrafish) (8)
Drer\kat8
13 of 14
Yes
Yes
Drer\kat5b
5 of 14
No
No
Drer\kat5a
4 of 14
No
No
Drer\kat6a
3 of 14
No
No
Drer\kat7b
3 of 14
No
No
Drer\kat6b
1 of 14
No
Yes
Drer\kat7a
1 of 14
No
No
Drer\samd1b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\mys-2
12 of 14
Yes
Yes
Cele\mys-1
5 of 14
No
No
Cele\mys-4
4 of 14
No
No
Cele\lsy-12
2 of 14
No
No
Cele\Y22D7AR.10
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (7)
Agam\AgaP_AGAP012229
11 of 12
Yes
Yes
Agam\AgaP_AGAP001539
4 of 12
No
No
Agam\AgaP_AGAP002231
3 of 12
No
No
Agam\AgaP_AGAP002735
3 of 12
No
No
Agam\AgaP_AGAP009676
2 of 12
No
No
Agam\AgaP_AGAP009677
2 of 12
No
No
Agam\AgaP_AGAP010081
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\HAM1
10 of 13
Yes
Yes
Atha\HAM2
10 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\ESA1
4 of 13
Yes
No
Scer\SAS2
3 of 13
No
Yes
Scer\SAS3
3 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\mst1
4 of 12
Yes
No
Spom\mst2
3 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:mof. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
Tip60
7 of 13
chm
6 of 13
Myst5
6 of 13
enok
5 of 13
d4
1 of 13
e(y)3
1 of 13
tth
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    mof2
    DOES NOT exacerbate  Huntington's disease
    modeled by Hsap\HTTQ93.ex1.UAS
    (Bodai et al., 2012)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KAT8; lysine acetyltransferase 8
    13 of 14
    609912
      KAT5; lysine acetyltransferase 5
      5 of 14
      601409
        KAT6A; lysine acetyltransferase 6A
        3 of 14
        601408
        KAT6B; lysine acetyltransferase 6B
        3 of 14
        605880
        KAT7; lysine acetyltransferase 7
        3 of 14
        609880
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            mof
            enzymatic study, autoradiography, anti tag coimmunoprecipitation, western blot
            (Mendjan et al., 2006, Buscaino et al., 2003)
            mof - Chd1
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            mof - Chro
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            mof - Dis3
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            mof - E(bx)
            anti bait coimmunoprecipitation, western blot
            (Lam et al., 2019)
            mof - His2A
            enzymatic study, autoradiography
            (Smith et al., 2000)
            mof - His3
            enzymatic study, autoradiography
            (Smith et al., 2000)
            mof - His4
            enzymatic study, autoradiography, histone acetylase assay, western blot, inferred by curator
            (Raja et al., 2010, Mendjan et al., 2006, Smith et al., 2000)
            mof - MBD-R2
            anti tag coimmunoprecipitation, Identification by mass spectrometry, coimmunoprecipitation, western blot, anti bait coimmunoprecipitation
            (Lam et al., 2019, Raja et al., 2010, Mendjan et al., 2006)
            mof - Mgtor
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
            (Aleman et al., 2021, Mendjan et al., 2006)
            mof - Nup153
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
            (Mendjan et al., 2006)
            mof - Nup98-96
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            mof - Polr2A
            anti bait coimmunoprecipitation, western blot
            (Andersen et al., 2010)
            mof - Rcd1
            anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, molecular weight estimation by staining
            (Meunier et al., 2015, Mendjan et al., 2006)
            mof - Rcd5
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Lam et al., 2019, Raja et al., 2010, Mendjan et al., 2006)
            mof - bon
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot
            (Godneeva et al., 2023)
            mof - dgt1
            anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Lam et al., 2019, Dias et al., 2014, Mendjan et al., 2006)
            mof - mle
            proximity ligation assay, fluorescence microscopy, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
            (Aleman et al., 2021, Schunter et al., 2017, Lindehell et al., 2015, Buscaino et al., 2003, Akhtar et al., 2000)
            mof - msl-1
            anti tag coimmunoprecipitation, western blot, pull down, anti bait coimmunoprecipitation, affinity technology, molecular weight estimation by staining, anti tag western blot, Identification by mass spectrometry, proximity ligation assay, fluorescence microscopy, autoradiography
            (Samata et al., 2020, Schunter et al., 2017, Chlamydas et al., 2016, Lindehell et al., 2015, Ilik et al., 2013, Conrad et al., 2012, Hallacli et al., 2012, Kadlec et al., 2011, Prabhakaran and Kelley, 2010, Raja et al., 2010, Mendjan et al., 2006, Morales et al., 2004, Scott et al., 2000, Smith et al., 2000)
            mof - msl-2
            anti tag coimmunoprecipitation, anti tag western blot, proximity ligation assay, fluorescence microscopy, western blot, enzymatic study, Identification by mass spectrometry, molecular weight estimation by staining, anti bait coimmunoprecipitation
            (Schunter et al., 2017, Lindehell et al., 2015, Hallacli et al., 2012, Villa et al., 2012, Mendjan et al., 2006, Straub et al., 2005, Akhtar et al., 2000, Smith et al., 2000)
            mof - msl-3
            anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry, proximity ligation assay, fluorescence microscopy, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, affinity technology, molecular weight estimation by staining, enzymatic study, autoradiography, experimental knowledge based
            (Samata et al., 2020, Schunter et al., 2017, Zee et al., 2016, Lindehell et al., 2015, Alekseyenko et al., 2014, Rhee et al., 2014, Conrad et al., 2012, Kadlec et al., 2011, Mendjan et al., 2006, Morales et al., 2004, Buscaino et al., 2003, Akhtar et al., 2000)
            mof - nsl1
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry, pull down, molecular weight estimation by staining
            (Lam et al., 2019, Dias et al., 2014, Kadlec et al., 2011, Raja et al., 2010, Mendjan et al., 2006)
            mof - pzg
            anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            mof - wds
            anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot
            (Raja et al., 2010, Mendjan et al., 2006)
            mof - αTub84B
            anti tag coimmunoprecipitation, Identification by mass spectrometry
            (Mendjan et al., 2006)
            RNA-protein
            Physical Interaction
            Assay
            References
            mof - lncRNA:roX1
            anti tag coimmunoprecipitation, northern blot
            (Mendjan et al., 2006)
            mof - lncRNA:roX2
            anti bait coimmunoprecipitation, reverse transcription pcr, anti tag coimmunoprecipitation, northern blot, primer specific pcr
            (Mendjan et al., 2006, Buscaino et al., 2003, Akhtar et al., 2000)
            Summary of Genetic Interactions
            esyN Network Diagram
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            esyN Network Key:
            Suppression
            Enhancement
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Iswi
            enhanceable
            mof
            (Corona et al., 2002)
            sc|sisA
            enhanceable
            mof
            (Gladstein et al., 2010)
            Top2
            suppressible
            mof
            (Mengoli et al., 2014)
            External Data
            Subunit Structure (UniProtKB)
            Component of the male-specific lethal (MSL) histone acetyltransferase complex, composed of mof, mle, msl-1, msl-2 and msl-3 proteins, as well as roX1 and roX2 non-coding RNAs (PubMed:10679323, PubMed:11014199, PubMed:16543150, PubMed:18510926, PubMed:20620953, PubMed:20620954, PubMed:22421046). Component of a maternal MSL subcomplex composed of mof, msl-1 and msl-3 (PubMed:32502394). Component of the non-specific lethal (NLS) histone acetyltransferase complex at least composed of mof, nls1, dgt1/NSL2, Rcd1/NSL3, Rcd5/MCRS2, MBD-R2 and wds (PubMed:16543150, PubMed:20620953, PubMed:20620954, PubMed:22723752). In males, interacts with nucleoporin Mtor (PubMed:34133927).
            (UniProt, O02193 )
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map
            1-14
            Cytogenetic map
            5C5-5C5
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            5C5-5C5
            Limits computationally determined from genome sequence between P{EP}CG15770EP390 and P{EP}Act5CEP1604&P{EP}EP444
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            5C3-5C3
            (Hilfiker, 1996.9.18)
            5C3-5C3
            (determined by in situ hybridisation)
            (Hilfiker et al., 1997)
            Experimentally Determined Recombination Data
            Location

            1-14

            (FlyBase, 2016)

            1-15.4

            (Comeron et al., 2012)

            1-13.8

            (Hilfiker et al., 1998, Hilfiker et al., 1995)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (11)
            Genomic Clones (18)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (40)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Riddle et al., 2012, Prestel et al., 2010, Smith et al., 2000)
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments

                  The MSL complex does not mediate dosage compensation directly, but rather its activity overrides the high level of histone acetylation and counteracts the potential overexpression of X-linked genes to achieve the proper twofold up-regulation in males.

                  (Sun et al., 2013)

                  mof protein distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2 protein, a global facilitator of transcription.

                  (Prestel et al., 2010)

                  Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

                  (Liu and Lehmann, 2008)

                  Gene products of the male specific lethal (msl) group of genes including msl-1, msl-2, msl-3, mle, and mof are associated with all female chromosomes at a low level but are sequestered to the X chromosome in males. There is evidence for the presence of nucleation sites for association of msl proteins with the X chromosome rather than individual gene binding sites. mof is still capable of associating with the chromosome in msl mutant individuals. It appears to be able to dissociate from other members of the MSL complex under these circumstances and act independently.

                  (Bhadra et al., 1999)

                  Gene products of the male specific lethal (msl) group of genes preferentially associate with the male X chromosome and may have a role in dosage compensation. This may be achieved by regulating an inverse dosage effect, which would be maintained on the male X and nullified on the autosomes.

                  (Bhadra et al., 1999)

                  mof colocalises with the MSL complex on the X chromosome: a sequence of binding events results in the formation of the MSL complex on the X chromosome in males and in the targeting of mof to its presumed site of action.

                  (Gu et al., 1998)

                  mof encodes a putative histone acetyl transferase (HAT) that plays a direct role in the specific histone acetylation associated with dosage compensation, the male-specific hypertranscription of X-linked genes. mof function in dosage compensation is supported by the effect of the mutation on the binding of other dosage compensation regulatory factors to the X chromosome and its effect on the normal consequences that this binding has on nucleosomal structure.

                  (Hilfiker et al., 1997)

                  Male lethal locus that exhibits a strong maternal effect and is responsible for dosage compensation in males.

                  (Hilfiker et al., 1995)
                  Relationship to Other Genes
                  Source for database merge of
                  Additional comments

                  The mof gene may have been derived from the chm gene by retroposition.

                  (Pan and Zhang, 2009)
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: mof CG3025

                  (Zhong, 1999.11)
                  Nomenclature comments
                  Etymology
                  Synonyms and Secondary IDs (14)
                  Reported As
                  Symbol Synonym
                  CG3025
                  (Meadows, 2017.1.11, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Ni et al., 2008, Kim et al., 2004)
                  MOF
                  (Richards et al., 2022, Aleman et al., 2021, Dai et al., 2021, Makki and Meller, 2021, Wolfe et al., 2021, Zhang et al., 2021, Samata et al., 2020, Bhardwaj et al., 2019, Lam et al., 2019, Leatham-Jensen et al., 2019, Lv et al., 2019, Mujoo et al., 2017, Cugusi et al., 2016, Zee et al., 2016, Keller and Akhtar, 2015, Lindehell et al., 2015, Lucchesi and Kuroda, 2015, Meunier et al., 2015, Saberi et al., 2015, Vensko and Stone, 2015, Alekseyenko et al., 2014, Chen et al., 2014, Chery and Larschan, 2014, Comoglio and Paro, 2014, Dias et al., 2014, Ferrari et al., 2014, Figueiredo et al., 2014, Ho et al., 2014, Lubelsky et al., 2014, McElroy et al., 2014, Zhimulev et al., 2014, Ilik et al., 2013, Philip and Stenberg, 2013, Conrad et al., 2012, Dunlap et al., 2012, Feller et al., 2012, Lam et al., 2012, Lv et al., 2012, Maenner et al., 2012, Philip et al., 2012, Villa et al., 2012, Georgiev et al., 2011, Morra et al., 2011, Regnard et al., 2011, Stenberg and Larsson, 2011, Straub and Becker, 2011, Andersen et al., 2010, Deng and Disteche, 2010, Raja et al., 2010, Schiemann et al., 2010, Vaquerizas et al., 2010, Grimaud and Becker, 2009, Patalano et al., 2009, Birchler et al., 2008, Kind et al., 2008, Straub et al., 2008, Sural et al., 2008, Mendjan and Akhtar, 2007, Morra et al., 2007, Park et al., 2007, Roy et al., 2007, Straub and Becker, 2007, Birchler et al., 2006, Buscaino et al., 2006, Dahlsveen et al., 2006, Deng and Meller, 2006, Deng and Meller, 2006, Furuhashi et al., 2006, Gilfillan et al., 2006, Park et al., 2006, de la Cruz et al., 2005, Li et al., 2005, Morales et al., 2005, Nielsen et al., 2005, Nusinow and Panning, 2005, Straub et al., 2005, Kelley, 2004, Kellum, 2003, Bouazoune et al., 2002, Corona et al., 2002, Akhtar and Becker, 2001, Akhtar and Becker, 2001, Rattner and Meller, 2001, Kelley and Kuroda, 2000, Scott et al., 2000, Smith et al., 2000, Kouzarides, 1999)
                  Mof
                  (Miao et al., 2022, Lauria Sneideman and Meller, 2021, Hallacli et al., 2012, Kadlec et al., 2011, Schwaiger et al., 2009, Kim et al., 2005, Kouzarides, 1998)
                  dMOF
                  (Bell et al., 2007, Li et al., 2007, Carrozza et al., 2003)
                  dmMOF
                  (Pandey et al., 2002)
                  max
                  (Hilfiker et al., 1998, Lucchesi, 1996, Hilfiker, 1995.5.26, Hilfiker et al., 1995)
                  mof
                  (Lee et al., 2024, Godneeva et al., 2023, Ding et al., 2022, Ota et al., 2021, Witt et al., 2021, Haberle et al., 2019, Luzhin et al., 2019, Molnar et al., 2019, Prayitno et al., 2019, Gene Disruption Project members, 2018-, Rennie et al., 2018, Valsecchi et al., 2018, Janssens et al., 2017, Schunter et al., 2017, Transgenic RNAi Project members, 2017-, Birchler, 2016, Chlamydas et al., 2016, Hirano et al., 2016, Ulianov et al., 2016, Koya and Meller, 2015, Lucchesi and Kuroda, 2015, Sun et al., 2015, dos Santos, 2014, Figueiredo et al., 2014, Mengoli et al., 2014, Czech et al., 2013, Philip and Stenberg, 2013, Pushpavalli et al., 2013, Sun et al., 2013, Vaque et al., 2013, Wang et al., 2013, Bateman et al., 2012, Bhadra et al., 2012, Bodai et al., 2012, Conrad et al., 2012, Conrad et al., 2012, Hainaut et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Larschan et al., 2012, Birchler et al., 2011, Friedman et al., 2011, Stenberg and Larsson, 2011, Gan et al., 2010, Gladstein et al., 2010, Prabhakaran and Kelley, 2010, Prestel et al., 2010, Schiemann et al., 2010, Yu et al., 2010, Zhang et al., 2010, Gelbart et al., 2009, Menon and Meller, 2009, Sun and Birchler, 2009, Bell et al., 2008, Kulkarni-Shukla et al., 2008, Li et al., 2008, Liu and Lehmann, 2008, Matyunina et al., 2008, Morra et al., 2008, Spierer et al., 2008, Kind and Akhtar, 2007, Levine et al., 2007, Rodriguez et al., 2007, Worringer and Panning, 2007, Yokoyama et al., 2007, Furuhashi et al., 2006, Mendjan et al., 2006, Pal Bhadra et al., 2006, Schubeler, 2006, Ray and Fox, 2005, Zhimulev et al., 2003, Gim et al., 2001)
                  Name Synonyms
                  MOF histone acetylase
                  (Sun and Birchler, 2007)
                  Males absent on first
                  (Deng and Meller, 2006)
                  Males absent on the first
                  (McElroy et al., 2014, Gim et al., 2001)
                  males absent on first
                  (Conrad et al., 2012, Prabhakaran and Kelley, 2010, Raja et al., 2010)
                  males absent on the first
                  (Mehrotra and Deshpande, 2018, Mujoo et al., 2017, Birchler, 2016, Kuroda et al., 2016, Comoglio and Paro, 2014, Dias et al., 2014, Bhadra et al., 2012, Birchler et al., 2011, Georgiev et al., 2011, Morra et al., 2011, Straub and Becker, 2011, Deng and Disteche, 2010, Kulkarni-Shukla et al., 2008, Birchler et al., 2006)
                  males-absent on the X
                  (Hilfiker, 1995.5.26)
                  males-absent-on-the-first
                  (Lv et al., 2012, Morales et al., 2005)
                  Secondary FlyBase IDs
                    Datasets (2)
                    Study focus (2)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    • bait_protein
                    modENCODE_chromatin_proteins_cells
                    Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
                    • bait_protein
                    modENCODE_chromatin_proteins_flies
                    Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 42 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    FlyMine - An integrated database for Drosophila genomics
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    PDB - An information portal to biological macromolecular structures
                    Linkouts
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    References (285)