zinc finger transcription factors - required for head segment identity during Drosophila embryogenesis - required for optic lobe development and ventral appendage development
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.43
Gene model reviewed during 5.53
2.9 (northern blot)
568 (aa); 62 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\disco using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: reported as gnathal lobes anlage
Comment: reference states >=6 hr AEL
At early third instar larval stage, disco is expressed in the antennal and maxillary palp regions of the eye-antennal disc, in the labial disc, and in leg discs. It is also expressed in a small group of cells in the future scutellum of the wing disc, in a similar position in the haltere disc, and weakly in the anterior-ventral fold of the eye disc.
disco transcripts are first detected at 6hr of embryonic development. They are detected throughout embryonic and larval stages and increase in abundance at each larval molt. Levels remain high in pupae and drop in adults where transcripts are detected at higher levels in head than body RNA.
The disco transcript is first detected in the posterior pole of the cellular blastoderm, and expression in then detected in anterior regions. Following head involution, expression is detected in the gnathal buds, labral and antennal segments as well as in the posterior of the procephalic lobe. Signal is also detected in the ectoderm of the thoracic segments, as well as in the visceral mesoderm. Following germ band retraction, expression in detected in the ventral nerve cord and in a sheet of cells surrounding the gut.
disco protein expression essentially recapitualtes the disco transcript expression pattern. In addition to the regions marked by in situ hybridization, disco antibody staining detects expression in the cardioblast cells which give rise to the dorsal vessel. A segmentally repeated pattern is also detected in the lateral ectoderm near the peripheral nervous system. Expression is also detected in tissues which give rise to the visual system.
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
New stable cell line derived from S2-unspecified : 75 stable S2 cell lines containing a library of intrinsically disordered regions (IDRs) fluorescently tagged with mNeonGreen were constructed. In addition, stable S2 cell lines expressing the following full-length transcription factors were generated: da, rib, CG10321, CG13287, tgo, Spps, CG31510, brk, and disco.
DNA-protein interactions: genome-wide binding profile assayed for disco protein in 0-12 hr embryos; see mE1_TFBS_disco collection report.
disco expression in the optic lobe primordium is dependant on an autoregulatory feedback loop.
Mutations disrupt neural cell patterning in the visual system, leading to the loss of many optic lobe neurons. The presence of a single ventral lateral neurons (LNV) is sufficient to provoke robust circadian rhythmicity in locomotor activity if the LNV terminals reach the superior protocerebrum.
The development of an identified serotonergic aborization that projects from the central brain has been analysed. Using mutations of disco that impair the connectivity of the larval optic nerve to the larval neuropil centre it is concluded that neither cell viability nor the initial outgrowth of this projection depends on the presence of the larval optic nerve. Aborization of the projection within the larval optic neuropil requires the presence of the larval optic nerve.
Genetic mosaic analysis demonstrates that R cell degeneration in disco mutant does not depend on the genotype of cells in the eye and is strictly correlated with the failure of R cells to project to the optic ganglia. Retinal degeneration can be rescued by the establishment of connections with disorganized optic lobes.
disco flies are rhythmic, indicating they have an active circadian pacemaker that can be entrained by light. Analysis of mutants suggests they block or interfere with elements of the circadian system located between the central pacemaker and its outputs that mediate overt rhythms.
Increasing the copy number of disco+ has no major effect on development.
disco mutants sing in an essentially normal manner, although their locomotor activity is largely arrhythmic.
Mutant analysis suggests that light normally triggers the coupling of multiple ultradian oscillators into a functional circadian clock and that this process is disrupted in disco mutant flies as a result of the neural lesion.
disco mutants have a characteristic deformed eye: the absence of the Bolwig nerve in larvae prevents retinula axons from finding their way to the optic lobe.
Mosaic analysis and fate mapping data concludes that the primary defect responsible for the adult disco phenotype is a failure of Bolwig's nerve to contact its correct target cells during embryonic development.
The adult phenotype of disco is caused by a pathfinding defect of a larval pioneer nerve. disco is required for the navigation of a specific subset of neurons in the peripheral nervous system. Phenotypic analyses and genetic mosaic experiments suggest that disco mutations affect the nerve rather than the target in the CNS or cells guiding the nerve's growth cone.
Not allelic to bss or eas, two closely linked behavioral mutants in 14B4-13; order = disco eas bss.
