FlyBase Allele Report: Dmel\sloth1[KO]

General Information

Symbol

Dmel\sloth1^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0386630

Feature type

allele

Associated gene

Dmel\sloth1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Bosch et al., 2022)

Mutagen

CRISPR/Cas9

(Bosch, 2023.8.3, Bosch et al., 2022)

Progenitor genotype

Cytology

Description

Two deletions (13bp and 12bp) in sloth1, resulting in a frameshift.

(Bosch, 2023.8.3)

A frame-shift indel (comprising deletions of 13bp and 12bp) within the sloth1 coding region.

(Bosch et al., 2022)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:7,011,615..7,011,627 [+]

Comment:

13bp in sloth1, resulting in a frameshift. One of two deletions in mutant. The second deletion of 12bp begins 20bp downstream.

(Bosch, 2023.8.3)

deletion

X:7,011,647..7,011,658 [+]

Comment:

12bp in sloth1. One of two deletions in mutant. The other deletion of 13bp ends 20bp upstream.

(Bosch, 2023.8.3)

delins

X:7,011,615..7,011,658 [+]

Inserted_sequence:

GTCGTCTGCTGGACAGTTG

Comment:

Two deletions of 13bp and 12bp separated by GTCGTCTGCTGGACAGTTG (equivalent of a 44bp deletion and insertion of GTCGTCTGCTGGACAGTTG) in the sloth1 coding region leads to a frameshift.

(Bosch et al., 2022)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased rate of adult locomotory behavior

(Bosch et al., 2022)

lethal

(Bosch, 2023.8.3)

lethal | male

(Bosch et al., 2022)

visible | adult stage

(Bosch et al., 2022)

Phenotype Manifest In

scutellar bristle

(Bosch et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sloth1^KO has lethal | male phenotype, suppressible by Scer\GAL4^da.PU/Hsap\UQCC5^UAS.cBa

(Bosch et al., 2022)

NOT suppressed by

Statement

Reference

sloth1^KO has lethal | male phenotype, non-suppressible by sloth2^+tBa

(Bosch et al., 2022)

sloth1^KO has lethal | male phenotype, non-suppressible by Scer\GAL4^da.PU/sloth2^UAS.cBa

(Bosch et al., 2022)

sloth1^KO has lethal | male phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\UQCC6^UAS.cBa

(Bosch et al., 2022)

Other

Statement

Reference

sloth1^KO, sloth2^KO has viable | female phenotype

(Bosch et al., 2022)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sloth1^KO is rescued by sloth1^+tBa.sloth2

(Bosch et al., 2022)

sloth1^KO is rescued by sloth1^+tBa

(Bosch et al., 2022)

sloth1^KO is rescued by Scer\GAL4^da.PU/sloth1^UAS.cBa

(Bosch et al., 2022)

sloth1^KO is rescued by sloth1^{UAS.sloth2.cBa}/Scer\GAL4^da.PU

(Bosch et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

94062

y¹ w^* sloth1^KO/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG32736²

(Bosch, 2023.8.3)

sloth1-KO

(Bosch et al., 2022)

sloth1^KO

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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