UASt regulatory sequences drive expression of a secretion signal from aos (MPTTLMLLPCMLLLLLTAAAVAVGG) followed by the CS6253 peptide where citrulline residues are replaced by arginine (EVRSKLEEWLAALRELAEELLARAKS). This peptide is an Hsap\ABCA1 agonist (drives Hsap\APOE lipidation). Tagged at the C-terminal with 3xTag:HA. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence. A loxP site is present upstream of UASt.
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\APOE4.R112.R158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a non-enhancer of lipid droplet | adult stage phenotype of Hsap\APOE2.C112.C158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a non-enhancer of pigment cell | adult stage phenotype of Hsap\APOE2.C112.C158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a non-enhancer of lipid droplet | adult stage phenotype of Hsap\APOE3.C112.R158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a non-enhancer of pigment cell | adult stage phenotype of Hsap\APOE3.C112.R158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a suppressor of rhabdomere | adult stage phenotype of Hsap\APOE4.R112.R158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0
Zzzz\aptamerCS6253.UAS.SS.Tag:HA, Scer\GAL4GLaz-MI02243-TG4.0 is a suppressor of retina | adult stage phenotype of Hsap\APOE4.R112.R158.UAS, MarfninaE.GD11094, Scer\GAL4GLaz-MI02243-TG4.0