FlyBase Allele Report: Dmel\promL[A3-1]

General Information

Symbol

Dmel\promL^A3-1

Species

D. melanogaster

Name

FlyBase ID

FBal0347581

Feature type

allele

Associated gene

Dmel\promL

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zheng et al., 2019)

Mutagen

CRISPR/Cas9

(Zheng et al., 2019)

Progenitor genotype

Cytology

Description

24bp deletion plus 23bp insertion within prominin-like. This results in a frameshift and a stop codon in the first exon.

(Zheng et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:3,120,875..3,120,898 [-]

Comment:

24 bases in the first coding exon of promL are deleted and replaced with 23 bases causing a frameshift and leading to early translation termination.

Inserted_sequence:

GGGCCGGTTGCAGATCGCGGGAG

(Zheng et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with promL^Δ)

(Zheng et al., 2019)

abnormal locomotor behavior | adult stage (with promL^Δ)

(Zheng et al., 2019)

abnormal neuroanatomy | adult stage (with promL^Δ)

(Zheng et al., 2019)

abnormal size | adult stage (with promL^Δ)

(Zheng et al., 2019)

abnormal starvation stress response | adult stage (with promL^Δ)

(Zheng et al., 2019)

decreased size | adult stage

(Hurbain et al., 2022)

decreased size | larval stage

(Hurbain et al., 2022)

fertile (with promL^Δ)

(Zheng et al., 2019)

increased body size | adult stage (with promL^Δ)

(Zheng et al., 2019)

increased body size | larval stage (with promL^Δ)

(Zheng et al., 2019)

increased body size | P-stage (with promL^Δ)

(Zheng et al., 2019)

long lived | calorie restriction conditional (with promL^Δ)

(Zheng et al., 2019)

viable

(Zheng et al., 2019)

visible | adult stage (with promL^Δ)

(Zheng et al., 2019)

visible | larval stage (with promL^Δ)

(Zheng et al., 2019)

visible | P-stage (with promL^Δ)

(Zheng et al., 2019)

Phenotype Manifest In

adult abdominal fat mass (with promL^Δ)

(Zheng et al., 2019)

epithelial cell of wing disc

(Hurbain et al., 2022)

lipid droplet | adult stage (with promL^Δ)

(Zheng et al., 2019)

microvillus | decreased number | larval stage

(Hurbain et al., 2022)

mushroom body calyx | adult stage (with promL^Δ)

(Zheng et al., 2019)

wing

(Hurbain et al., 2022)

wing disc

(Hurbain et al., 2022)

wing pouch

(Hurbain et al., 2022)

Detailed Description

Statement

Reference

In promL^A3-1 larvae, the wing disc pouch is small, and microvilli are absent or severely shortened/misshapen.

(Hurbain et al., 2022)

In promL^Δ/promL^A3-1 adults the body weight is significantly increased, the average daytime locomotor activity is decreased and the size of the mushroom body calyx (FAS II) is decreased while during development there is no alteration in neuroblast asymmetric division (Miranda) at third instar larval stage or in the developmental timing when compared to controls.

promL^Δ/promL^A3-1 animals, on a normal diet, exhibit a significant increase in body size at pupal and adult stages; they exhibit an increase in lipid droplet size in the adult abdominal fat body, but not on the third instar larval fat body when compared to controls.

In promL^Δ/promL^A3-1 animals on the low protein diet the pupation rate is significantly greater, the eclosion time is significantly shorter, the adult body is significantly heavier and both larvae and prepupae are larger than controls.

(Zheng et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

promL^Δ/promL^A3-1 has increased body size | pupal stage phenotype, suppressible by Scer\GAL4^Cg.PU/Hsap\PROM1^UAS.cZa

(Zheng et al., 2019)

promL^Δ/promL^A3-1 has visible | P-stage phenotype, suppressible by Scer\GAL4^Cg.PU/Hsap\PROM1^UAS.cZa