FlyBase Allele Report: Dmel\promL[Δ]

Approximately 11.4kb deletion that removes the majority of the prominin-like gene (the entire coding sequence is removed). Generated by FLP mediated recombination between the two progenitor insertions (PBac{RB}prominin-like^e03410 and PBac{WH}CG11537^f01754); a hybrid PBac{RB5.WH5} element remains in place of the deleted sequence.

(Zheng et al., 2019)

Allele components

Component

Use(s)

Inserted element

PBac{RB5.WH5}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

complex_substitution

3L:3,115,802..3,128,051 [-]

Comment:

Deletion generated by FLP-mediated recombination between two progenitor insertions that removes the promL coding sequence; a hybrid PBac{RB5.WH5} element remains in place of the deleted sequence. The estimated deletion breakpoints correspond to the insertion sites of the progenitor insertions.

(Zheng et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with promL^A3-1)

(Zheng et al., 2019)

abnormal locomotor behavior | adult stage (with promL^A3-1)

(Zheng et al., 2019)

abnormal neuroanatomy | adult stage (with promL^A3-1)

(Zheng et al., 2019)

abnormal size | adult stage (with promL^A3-1)

(Zheng et al., 2019)

abnormal starvation stress response | adult stage (with promL^A3-1)

(Zheng et al., 2019)

fertile (with promL^A3-1)

(Zheng et al., 2019)

increased body size | adult stage (with promL^A3-1)

(Zheng et al., 2019)

increased body size | larval stage (with promL^A3-1)

(Zheng et al., 2019)

increased body size | P-stage (with promL^A3-1)

(Zheng et al., 2019)

long lived | calorie restriction conditional (with promL^A3-1)

(Zheng et al., 2019)

viable

(Zheng et al., 2019)

visible | adult stage (with promL^A3-1)

(Zheng et al., 2019)

visible | larval stage (with promL^A3-1)

(Zheng et al., 2019)

visible | P-stage (with promL^A3-1)

(Zheng et al., 2019)

Phenotype Manifest In

adult abdominal fat mass (with promL^A3-1)

(Zheng et al., 2019)

lipid droplet | adult stage (with promL^A3-1)

(Zheng et al., 2019)

mushroom body calyx | adult stage (with promL^A3-1)

(Zheng et al., 2019)

Detailed Description

Statement

Reference

In promL^Δ/promL^A3-1 adults the body weight is significantly increased, the average daytime locomotor activity is decreased and the size of the mushroom body calyx (FAS II) is decreased while during development there is no alteration in neuroblast asymmetric division (Miranda) at third instar larval stage or in the developmental timing when compared to controls.

promL^Δ/promL^A3-1 animals, on a normal diet, exhibit a significant increase in body size at pupal and adult stages; they exhibit an increase in lipid droplet size in the adult abdominal fat body, but not on the third instar larval fat body when compared to controls.

In promL^Δ/promL^A3-1 animals on the low protein diet the pupation rate is significantly greater, the eclosion time is significantly shorter, the adult body is significantly heavier and both larvae and prepupae are larger than controls.

(Zheng et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

promL^Δ/promL^A3-1 has increased body size | pupal stage phenotype, suppressible by Scer\GAL4^Cg.PU/Hsap\PROM1^UAS.cZa

(Zheng et al., 2019)

promL^Δ/promL^A3-1 has visible | P-stage phenotype, suppressible by Scer\GAL4^Cg.PU/Hsap\PROM1^UAS.cZa