FlyBase Allele Report: Dmel\Kdm3[KO]

General Information

Symbol

Dmel\Kdm3^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0335397

Feature type

allele

Associated gene

Dmel\Kdm3

Associated Insertion(s)

TI{TI}Kdm3^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Shalaby et al., 2017)

Mutagen

ends-out gene targeting

(Shalaby et al., 2017)

FLPase

(Shalaby et al., 2017)

I-SceI

(Shalaby et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}Kdm3^KO

Cytology

Description

The endogenous JHDM2 open reading frame has been replaced by a loxP cassette which contains both Disc\RFP^3xP3.PC and the kanamycin resistance gene. A portion of the JHDM2 ORF remains in an exogenous location within the targeting vector.

(Shalaby et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:9,518,275..9,520,839 [+]

Comment:

The Kdm3 ORF was replaced with a knock-in cassette containing a Red Fluorescent Protein (RFP) transgene. Replaced region determined from reported primer sequences.

(Shalaby et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Pinzón et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation, with In(1)w^m4

(Shalaby et al., 2017)

enhancer of variegation, with T(2;3)Sb^V

(Shalaby et al., 2017)

viable

(Shalaby et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

JHDM2^KO mutants are viable and fertile and do not display any developmental timing defects but they do enhance the variegated phenotype of the w or Sb locus in the In(1)w^m4 or T(2;3)Sb^V background, respectively.

(Shalaby et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

JHDM2[+]/Kdm3^KO is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Suppressor of

Statement

Reference

JHDM2[+]/Kdm3^KO is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

JHDM2[+]/Kdm3^KO is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

JHDM2[+]/Kdm3^KO is a non-enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Suppressor of

Statement

Reference

JHDM2[+]/Kdm3^KO is a suppressor | partially of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

JHDM2[+]/Kdm3^KO is a non-suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

JHDM2[+]/Kdm3^KO is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Kdm4A^KO;JHDM2^KO as well as Kdm4B^KO;JHDM2^KO double mutants are viable and fertile.

Combination with a single copy of JHDM2^KO does not modify the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel nor the massive eye overgrowth in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} with Scer\GAL4^GMR.PF but it ameliorates the disrupted wing vein phenotype of flies expressing smo^{5A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^C-765.

(Shalaby et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

JHDM2^KO

KDM3^KO

(Shalaby et al., 2018, Pinzón et al., 2017, Shalaby et al., 2017)

Kdm3^KO

(Casier et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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