FB2024_03 , released June 25, 2024
Allele: Dmel\VincCO.UASp.TagRFP
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General Information
Symbol
Dmel\VincCO.UASp.TagRFP
Species
D. melanogaster
Name
FlyBase ID
FBal0325478
Feature type
allele
Associated gene
Dmel\Vinc
Associated Insertion(s)
Carried in Construct
P{UASp-Vinc.CO.TagRFP}
Also Known As
vinc-CO
Key Links
Transgenic product class
missense_variant
(Maartens et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Maartens et al., 2016)
Mutagen
in vitro construct
(Maartens et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-Vinc.CO.TagRFP}
Cytology
Description

UASp regulatory sequences drive expression of a constitutively open form of Vinc (full-length protein in which the 'T12' cluster of charged residues in the tail - residues 869-873 - have been mutated, from DRRIR to AAAIA) tagged at the C-terminal end with TagRFP.

(Maartens et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Vinc
Tagged with
TagRFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of either Scer\GAL4Act5C.PU or Scer\GAL4Mef2.PU leads to lethality; expression under the control of Scer\GAL4Mef2.PU results in a range of embryonic muscle defects in the majority of cases, including misshapen or misplaced muscles, gaps in the muscle pattern, and ectopic attachments, and to the formation of ectopic VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates in embryonic muscles; clonal expression under the control of Scer\GAL4Act5C.PU in the follicular and pupal wing epithelia also leads to the formation of ectopic VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates, as compared to controls.

      (Maartens et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaC110

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaΔ486-1937.GFP, Df(3L)79a/Df(3L)79a

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaΔhead.GFP, Df(3L)79a/Df(3L)79a

      (Maartens et al., 2016)

      Scer\GAL4Mef2.PU, VincCO.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by rheaC110

      (Maartens et al., 2016)

      Scer\GAL4Mef2.PU, VincCO.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by rheaVBS1.UASp.GFP/Scer\GAL4Mef2.PU

      (Maartens et al., 2016)

      Scer\GAL4Mef2.PU, VincCO.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by Scer\GAL4Mef2.PU/rheaVBS2.UASp.GFP

      (Maartens et al., 2016)

      Scer\GAL4Mef2.PU, VincCO.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by rheaIBS2.UAS.mGFP6/Scer\GAL4Mef2.PU

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by mys11

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible by rheaB28

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible by rheaA109

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible by rheaB128

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaB7

      (Maartens et al., 2016)

      Scer\GAL4Act5C.PU, VincCO.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaC18

      (Maartens et al., 2016)
      NOT suppressed by
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality induced by the expression of VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of Scer\GAL4Mef2.PU is suppressed by the co-expression of either rheaIBS2.Scer\UAS.T:Avic\GFP-m6, rheaVBS1.Scer\UAS.P\T.T:Avic\GFP or rheaVBS2.Scer\UAS.P\T.T:Avic\GFP. The frequency and severity of embryonic muscle defects induced by the expression of VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of Scer\GAL4Mef2.PU are increased by the co-expression of PaxScer\UAS.cZa, and decreased by the co-expression of rheaIBS2.Scer\UAS.T:Avic\GFP-m6, but are not significantly affected by the co-expression of rheaScer\UAS.cTa.

      The ectopic VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates formed in embryonic muscles upon Scer\GAL4Mef2.PU-driven expression are nearly abrogated by the co-expression of either one copy of rheaVBS1.Scer\UAS.P\T.T:Avic\GFP, one copy of rheaVBS1.Scer\UAS.P\T.T:Disc\RFP-mCherry, two copies of rheaVBS2.Scer\UAS.P\T.T:Avic\GFP or one copy of rheaIBS2.Scer\UAS.T:Avic\GFP-m6, but are not significantly affected by the co-expression of either PaxScer\UAS.cZa or rheaScer\UAS.cTa. The ectopic VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates formed upon Scer\GAL4Act5C.PU-driven expression in follicle cell clones are nearly abrogated by either rheaB28, rheaA109 or rheaB128 homozygosity, strongly reduced by either rheaB7, rheaC18 or rheaC110 homozygosity and moderately reduced by mys11 homozygosity, but not reduced by PaxΔ1 homozygosity; these ectopic aggregates in follicle cell clones are also reduced if the clones are also homozygous for Df(3L)79a and the individuals express rheaΔ486-1937.T:Avic\GFP (leading to a strong reduction of aggregates) and rheaΔhead.T:Avic\GFP (leading to a moderate reduction of aggregates).

      (Maartens et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      VincCO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP
      VincCO.UASp.TagRFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)