FB2024_03 , released June 25, 2024
Allele: Dmel\Eip78CΔ31
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General Information
Symbol
Dmel\Eip78CΔ31
Species
D. melanogaster
Name
FlyBase ID
FBal0319596
Feature type
allele
Associated gene
Dmel\Eip78C
Associated Insertion(s)
PBac{RBr}Eip78CΔ31
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Ables et al., 2015)
Mutagen
FLPase
(Ables et al., 2015)
Progenitor genotype
PBac{RB}Eip78Ce02853
(Ables et al., 2015)
PBac{RB}e02923
(Ables et al., 2015)
Associated Insertion(s)
PBac{RBr}Eip78CΔ31
Cytology
Description

Deletion of approximately 31kb of genomic sequence that removes the predicted translation start site of all Eip78C isoforms and the DNA-binding domain of isoforms RA/RD/RE. Generated by FLP-mediated recombination between the two progenitor insertions (PBac{RB}e02923 and PBac{RB}Eip78Ce02853).

(Ables et al., 2015)
Allele components
Component
Use(s)
Inserted element
PBac{RBr}
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eip78CΔ31/Eip78CΔ31 animals are viable and male fertile but completely female sterile.

      The number of eggs laid by the Eip78CΔ31/Df(3L)BSC419 transheterozygote females as well as the proportion of laid eggs that actually hatch is significantly decreased compared to age-matched controls. Majority of embryos derived from Eip78CΔ31 females fails to gastrulate.

      Eip78CΔ31/Df(3L)BSC419 transheterozygotes show increased developmental lethality when raised on a sugar-rich (K12 media) diet, which can be rescued by addition of yeast paste.

      Eip78CΔ31/Df(3L)BSC419 transheterozygote females have on average fewer germline stem cells (GSC) per germarium and show higher proportion of germaria containing less than three GSCs compared to age-matched controls. However, the rate of proliferation of GSCs and their maintenance over time is not perturbed in Eip78CΔ31 mutants compared to controls.

      Eip78CΔ31/Df(3L)BSC419 transheterozygote females show slight but significant decrease in the average number of cap cells per germarium, regardless of age of the female.

      The proportion of ovarioles containing dying follicles (both early and late stages) is highly increased in Eip78CΔ31/Df(3L)BSC419 transheterozygote females compared to wild-type as well as in mosaic animals with ovarioles containing Eip78CΔ31/Eip78CΔ31 germline clones. Stage 4/5 Eip78CΔ31 mutant cysts are significantly overrepresented, while stage 8-10 mutant cysts are significantly underrepresented in ovarioles containing Eip78CΔ31 germline clones compared to controls.

      (Ables et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The levels of follicle death (scored as percentage of ovarioles containing at least one dying follicle) in Eip78CΔ31;EcRM554fs double heterozygote females is significantly increased compared to either Eip78CΔ31/+ or EcRM554fs/+ alone.

      The levels of follicle death (scored as percentage of ovarioles containing at least one dying follicle) in Eip78CΔ31;ecd1 double heterozygote females is significantly increased compared to either Eip78CΔ31/+ or ecd1/+ alone.

      The levels of follicle death (scored as percentage of ovarioles containing at least one dying follicle) in Eip78CΔ31;Eip75BΔ51 double heterozygote females is not significantly increased compared to either Eip78CΔ31/+ or Eip75BΔ51/+ alone.

      (Ables et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Df(3L)BSC419/Eip78CΔ31 is partially rescued by Eip78CsfGFP,Tag:FLAG

      (Ables et al., 2015)
      Comments

      The decreased number of cap cells per germarium and the high proportion of ovarioles that contain dying follicles characteristic for Eip78CΔ31/Df(3L)BSC419 adult females can be partially restored by combination with single copy of Eip78CT:Avic\GFP-SF,T:Zzzz\FLAG but the reduced number of germline stem cells per germarium is not rescued.

      (Ables et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      E78Δ31
      (Ables et al., 2015)
      Eip78CΔ31
      Name Synonyms
      Secondary FlyBase IDs
        References (1)