FB2024_03 , released June 25, 2024
Allele: Dmel\Eip78CsfGFP,Tag:FLAG
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General Information
Symbol
Dmel\Eip78CsfGFP,Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0268856
Feature type
allele
Associated gene
Dmel\Eip78C
Associated Insertion(s)
Carried in Construct
PBac{Eip78C-GFP.FLAG}
Key Links
Transgenic product class
genomic_DNA
(Spokony and White, 2012.5.22)
wild_type
(Spokony and White, 2012.5.22)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Spokony and White, 2012.5.22)
wild_type
(Spokony and White, 2012.5.22)
Mutagen
in vitro construct
(Spokony and White, 2012.5.22)
Progenitor genotype
Carried in construct
PBac{Eip78C-GFP.FLAG}
Cytology
Description

Genomic fragment including the Eip78C gene, from BAC clone CH321-05P13. A sfGFP-Tag:FLAG tag cassette has been introduced at the C-terminal end of the Eip78C gene, such that protein isoforms A, B and C are tagged.

(Spokony and White, 2012.5.22)
Allele components
Component
Use(s)
Regulatory region(s)
Eip78C
Encoded product / tool
Eip78C
Tagged with
sfGFP
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | femaleoogenesis stage S1 -- S10
      nurse cell
      • nucleus
      (Ables et al., 2015)
      adult stage | femaleoogenesis stage S8 -- S12
      main body follicle cell
      • nucleus
      (Ables et al., 2015)
      border follicle cell
      • nucleus
      (Ables et al., 2015)
      Additional Information
      Statement
      Reference

      Eip78CsfGFP,Tag:FLAG protein is detected in the nuclei of nurse cells beginning in germarium regions 2b/3, and continuing through oogenesis stage S10. Eip78CsfGFP,Tag:FLAG protein is also detected in follicle cells, including in oocyte associated (main body) follicle cells, and in border follicle cells.

      (Ables et al., 2015)
       
      Marker for
      Reflects expression of
      Eip78C
      (Ables et al., 2015)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Eip78CsfGFP,Tag:FLAG partially rescues Df(3L)BSC419/Eip78CΔ31

      (Ables et al., 2015)
      Comments

      The decreased number of cap cells per germarium and the high proportion of ovarioles that contain dying follicles characteristic for Eip78CΔ31/Df(3L)BSC419 adult females can be partially restored by combination with single copy of Eip78CT:Avic\GFP-SF,T:Zzzz\FLAG but the reduced number of germline stem cells per germarium is not rescued.

      (Ables et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Eip78CT:Avic\GFP-SF,T:Zzzz\FLAG
      Eip78CsfGFP,Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)