FB2024_03 , released June 25, 2024
Allele: Dmel\ktub1
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General Information
Symbol
Dmel\ktub1
Species
D. melanogaster
Name
FlyBase ID
FBal0319122
Feature type
allele
Associated gene
Dmel\ktub
Associated Insertion(s)
TI{TI}ktub1
Carried in Construct
Also Known As
dTulp1
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Park et al., 2013)
Mutagen
ends-out gene targeting
(Park et al., 2013)
Progenitor genotype
Associated Insertion(s)
TI{TI}ktub1
Cytology
Description

Deletion of residues 220-460 of the ktub coding region (this includes sequence encoding the conserved "tubby" domain). A w+mW.hs marker has been inserted in place of the deleted ktub sequence.

(Park et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ktub1/ktub1 or ktub1/ktubG flies show significant locomotor defects (reduced climbing ability) compared to wild type. ktub1/ktub1 or ktub1/Df(2R)BSC462 flies show a significant reduction in sound evoked potentials from the antennal nerve compared to wild type. ktub1/ktub1 flies show an overall normal Johnston's organ structure, though electron microscopy reveals an abnormal number of chordotonal cilia (in around 6% of scolopidia) and cilia are found outside the cap structure (in around 3% of scolopidia).

      (Park et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      INPP5EMI02221/INPP5EMI02221 ktub1/ktub1 flies with expression of ktubScer\UAS.cPa or ktubmutB.Scer\UAS driven by Scer\GAL4nan.PK show significantly reduced amplitude of sound-evoked potentials from antennal nerves compared to ktub1/ktub1 Scer\GAL4nan.PK>ktubScer\UAS.cPa flies (similar to wild type amplitude); however, INPP5EMI02221/INPP5EMI02221 ktub1/ktub1 flies with expression of ktubmutB.Scer\UAS have a significantly increased amplitude in comparison to those with expression of ktubScer\UAS.cPa.

      (Park et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      ktub1 is rescued by ktubUAS.cPa/Scer\GAL4nan.PK

      (Park et al., 2015)

      ktub1 is rescued by ktub+tCH321-59C17

      (Park et al., 2013)

      ktub1 is rescued by ktubUAS.cPa/Scer\GAL4nan.PK

      (Park et al., 2013)
      Partially rescued by

      ktub1 is partially rescued by ktubmutA.UAS/Scer\GAL4nan.PK

      (Park et al., 2013)

      ktub1 is partially rescued by Scer\GAL4nan.PK/ktubmutB.UAS

      (Park et al., 2013)
      Not rescued by

      ktub1 is not rescued by Scer\GAL4nan.PK/ktubmutB.UAS

      (Park et al., 2015)

      ktub1 is not rescued by ktubmutAB.UAS/Scer\GAL4nan.PK

      (Park et al., 2013)
      Comments

      Expression of ktubScer\UAS.cPa (but not ktubmutB.Scer\UAS) driven by Scer\GAL4nan.PK leads to amplitude of sound-evoked potentials from ktub1/ktub1 antennal nerves being similar to controls.

      (Park et al., 2015)

      ktub+tCH321-59C17 rescues locomotor and auditory defects in ktub1/ktub1 flies. Expression of ktubScer\UAS.cPa fully rescues, ktubmutA.Scer\UAS or ktubmutB.Scer\UAS partially rescues, and ktubmutAB.Scer\UAS fails to rescue auditory defects in ktub1/ktub1 flies when driven by Scer\GAL4nan.PK .

      (Park et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dTulp1
      (Kwon et al., 2020, Park et al., 2015, Park et al., 2013)
      ktub1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)