FB2024_03 , released June 25, 2024
Allele: Dmel\FrlDN.UAS
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General Information
Symbol
Dmel\FrlDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0318783
Feature type
allele
Associated gene
Dmel\Frl
Associated Insertion(s)
Carried in Construct
P{UAS-Frl.DN}
Key Links
Transgenic product class
dominant_negative_variant
(Dollar et al., 2016)
inframe_deletion
(Dollar et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Dollar et al., 2016)
inframe_deletion
(Dollar et al., 2016)
Mutagen
in vitro construct
(Dollar et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Frl.DN}
Cytology
Description

UASt regulates expression of dominant negative (DN) Frl through deletion of the FH2 domain and C terminus (ΔFH2).

(Dollar et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Frl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of FrlDN.Scer\UAS driven by Scer\GAL4hs.2sev causes a severe rough eye phenotype; when driven by Scer\GAL4sev.EP there are ommatidial rotation defects (planar cell polarity-like defects) in adult eyes, along with the loss of R-cell clusters (co-expression of FrlCA.Scer\UAS does not alter the phenotype). Third instar larvae with expression of FrlDN.Scer\UAS driven by Scer\GAL4sev.EP have misrotated ommatidial clusters and clusters lacking R-cells in eye discs.

      (Dollar et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Statement
      Reference

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by nmoP1

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by shg1

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by shgk03401

      (Dollar et al., 2016)

      FrlDN.UAS, Scer\GAL4sev.EP has ommatidium phenotype, non-enhanceable by nmoDB

      (Dollar et al., 2016)
      Suppressed by
      Enhancer of
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      R-cell loss in flies with Cdc42Scer\UAS.cWa driven by Scer\GAL4sev.EP is completely suppressible with co-expression of FrlDN.Scer\UAS.

      Co-expression of Cdc42Scer\UAS.cWa suppresses the ommatidial rotation phenotypes (planar cell polarity-like defects) seen in flies with FrlDN.Scer\UAS driven by Scer\GAL4sev.EP. Presence of Cdc421/+ enhances the ommatidial rotation phenotypes and increases R-cell loss seen eyes of in flies with FrlDN.Scer\UAS driven by Scer\GAL4sev.EP.

      Expression of FrlDN.Scer\UAS driven by Scer\GAL4sev.EP enhances (likely additive) eye phenotypes (ommatidial rotation defects and R-cell loss) in flies with Rac1V12.Scer\UAS driven by Scer\GAL4hs.2sev.

      Presence of shg1, shgk03401, nmoDB or nmoP1 does not significantly alter eye phenotypes seen in flies with FrlDN.Scer\UAS driven by Scer\GAL4sev.EP.

      Presence of Df(2L)CadNΔ14/+ or Df(2L)BSC148/+ enhances ommatidial rotation (planar cell polarity-like defects) seen in flies with FrlDN.Scer\UAS driven by Scer\GAL4sev.EP.

      (Dollar et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      FrlDN.Scer\UAS
      FrlDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)