FlyBase Allele Report: Dmel\nmo[DB]

General Information

Symbol

Dmel\nmo^DB

Species

D. melanogaster

Name

FlyBase ID

FBal0260897

Feature type

allele

Associated gene

Dmel\nmo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Mirkovic et al., 2011)

Mutagen

Delta2-3 transposase

(Mirkovic et al., 2011)

Progenitor genotype

P{lacW}nmo^P1

(Mirkovic et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of nucleotides 44-1198 of the nmo transcript (this removes the predicted start codon at position 1035 and the following 54 amino acids).

(Mirkovic et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:7,979,636..7,980,982 [+]

Comment:

Inferred coordinates of a deletion resulting from the imprecise excision of P{lacW}nmo^P1 that is reported as a deletion of nucleotides 44-1198 of the nmo transcript that removes the predicted start codon at position 1035 and the following 54 amino acids.

(Mirkovic et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity

(Mirkovic et al., 2011)

abnormal planar polarity | somatic clone | third instar larval stage

(Mirkovic et al., 2011)

lethal - all die before end of P-stage

(Mirkovic et al., 2011)

some die during pharate adult stage

(Mirkovic et al., 2011)

Phenotype Manifest In

ommatidial precursor cluster | somatic clone

(Mirkovic et al., 2011)

ommatidium

(Mirkovic et al., 2011)

ommatidium | somatic clone

(Mirkovic et al., 2011)

wing vein | ectopic

(Mirkovic et al., 2011)

Detailed Description

Statement

Reference

Mutant die as pharate adults, with a low frequency of escapers that show ectopic vein material.

Homozygous clones in the third larval instar eye disc show severe under-rotation of ommatidial precursors.

(Mirkovic et al., 2011)

Mutant ommatidia show severely reduced rotation of ommatidia compared to controls (in many cases, clusters do not rotate at all). Clonal analysis indicates that the requirement for nmo[+] in ommatidial rotation is not restricted to any particular photoreceptor cell subtype, nmo[+] is required in a cluster-autonomous manner and more normal rotation correlates with the number of wild-type photoreceptor cells in a mosaic ommatidium.

(Mirkovic et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

nmo^DB is a non-enhancer of abnormal planar polarity | adult stage phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Suppressor of

Statement

Reference

nmo^DB/nmo[+] is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, gish^GD10588

(Weber et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

nmo^DB is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

nmo^DB is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

NOT Enhancer of

Statement

Reference

nmo^DB is a non-enhancer of ommatidium phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Suppressor of

Statement

Reference

nmo^DB/nmo[+] is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, gish^GD10588

(Weber et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of nmo^DB does not significantly alter eye phenotypes seen in flies with Frl^DN.Scer\UAS driven by Scer\GAL4^sev.EP.

(Dollar et al., 2016)

Dosage reduction of nmo, through a nmo^DB mutant background is able to enhance the eye roughness seen in Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

A nmo^DB mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

(Muñoz-Soriano et al., 2013)

nmo^DB/+ enhances the ommatidial rotation defects induced by Scer\GAL4^hs.2sev>gish^GD10588.

(Weber et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nmo^P1/nmo^DB is rescued by nmo^II.tub.GFP

(Mirkovic et al., 2011)

Partially rescued by

nmo^P1/nmo^DB is partially rescued by nmo^UAS.cUa/Scer\GAL4^hs.2sev

(Mirkovic et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nmo^DB24

(Mirkovic et al., 2011)

nmo^DB

(Thuveson et al., 2019, Collu et al., 2018, Dollar et al., 2016, Muñoz-Soriano et al., 2013, Weber et al., 2012, Mirkovic et al., 2011, Mirkovic et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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