FlyBase Allele Report: Dmel\Cdc42[1]

General Information

Symbol

Dmel\Cdc42¹

Species

D. melanogaster

Name

FlyBase ID

FBal0062066

Feature type

allele

Associated gene

Dmel\Cdc42

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Fehon et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: G10D. Nucleotide substitution: G50A.

(Genova et al., 2000)

Amino acid replacement: G10D.

(Fehon et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:19,697,986..19,697,986 [+]

Nucleotide change:

G19697986A

Reported nucleotide change:

G50A

Amino acid change:

G10D | Cdc42-PA; G10D | Cdc42-PC; G10D | Cdc42-PD

Reported amino acid change:

G10D

(Genova et al., 2000, Fehon et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Pagliarini and Xu, 2003)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Genova et al., 2000, Fehon et al., 1997)

visible (with Cdc42⁵)

(Genova et al., 2000)

Phenotype Manifest In

anterior crossvein | ectopic

(Genova et al., 2000)

anterior crossvein | ectopic (with Cdc42⁵)

(Genova et al., 2000)

eye (with Cdc42⁵)

(Genova et al., 2000)

follicle cell | somatic clone

(Genova et al., 2000)

interommatidial bristle (with Cdc42⁵)

(Genova et al., 2000)

ommatidium (with Cdc42⁵)

(Genova et al., 2000)

wing (with Cdc42⁵)

(Genova et al., 2000)

Detailed Description

Statement

Reference

Cdc42¹/+ fly eyes do not show R-cell loss or ommatidial rotation defects (planar cell polarity-like defects).

(Dollar et al., 2016)

12% of flies heterozygous for Cdc42¹ exhibit extra crossveins. Cdc42¹/Cdc42⁵ mutant eyes display disruptions in the normal ommatidial pattern, ommatidial fusion, and loss of bristles in the dorsal posterior quadrant. All photoreceptor cells are present within the ommatidia and in the proper orientation. Cdc42¹/Cdc42⁵ mutant wings often have extra anterior crossveins. Somatic Cdc42¹ clones in the follicle cells leads to the loss of columnar cell shape, formation of multiple layers of cells at the posterior end of the egg chamber, and apparent fusion of adjacent egg chambers.

(Genova et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cdc42¹/Cdc42[+] is an enhancer of abnormal planar polarity | adult stage phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Cdc42¹/Cdc42[+] is an enhancer of decreased cell number | adult stage phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Cdc42¹/Cdc42¹ is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Pagliarini and Xu, 2003)

NOT Enhancer of

Statement

Reference

Cdc42¹ is a non-enhancer of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

NOT Suppressor of

Statement

Reference

Cdc42¹ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Cdc42¹ is a non-suppressor of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Cdc42¹ has phenotype, non-suppressible by Mer^hs.PM

(Fehon et al., 1997)

Enhancer of

Statement

Reference

Cdc42¹/Cdc42[+] is an enhancer of ommatidium phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Cdc42¹/Cdc42[+] is an enhancer of eye photoreceptor cell phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

Cdc42¹/Cdc42[+] is an enhancer of eye phenotype of Frl^DN.UAS, Scer\GAL4^sev.EP

(Dollar et al., 2016)

NOT Suppressor of

Statement

Reference

Cdc42¹ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Cdc42¹/+ enhances the ommatidial rotation phenotypes and increases R-cell loss seen in eyes of flies with Frl^DN.Scer\UAS driven by Scer\GAL4^sev.EP.

(Dollar et al., 2016)

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa cannot be suppressed by combination with Cdc42¹ in heterozygous state.

(Ruan et al., 2016)

The Cdc42¹ mutation fails to modify the cv-c^M62 phenotype in the Malpighian tubules.

(Denholm et al., 2005)

Marked clones in the eye disc expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI and which are also homozygous for Cdc42¹ show metastatic behaviour.

(Pagliarini and Xu, 2003)

Not rescued by Mer^hs.PM.

(Fehon et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cdc42¹ is rescued by Cdc42^Ubi-p63E.PF

(Fehon et al., 1997)

Partially rescued by

Cdc42¹ is partially rescued by Cdc42^{Ubi-p63E.Tag:MYC}

(Genova et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8783

y¹ w^* Cdc42¹/FM6

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF153423

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAD43787

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdc42¹

(Dollar et al., 2016, Popodi et al., 2010-)

cdc42¹

(Ruan et al., 2016, Denholm et al., 2005, Pagliarini and Xu, 2003, Asha et al., 1999, Sawamoto et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close