FlyBase Allele Report: Dmel\SkpA[GD65]

General Information

Symbol

Dmel\SkpA^GD65

Species

D. melanogaster

Name

FlyBase ID

FBal0297325

Feature type

allele

Associated gene

Dmel\SkpA

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Brace et al., 2014)

Mutagen

ethyl methanesulfonate

(Brace et al., 2014)

Progenitor genotype

Cytology

Description

Amino acid replacement: E?K.

(Brace et al., 2014)

Nucleotide substitution: G94A.

(Brace et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:657,203..657,203 [+]

Nucleotide change:

G657203A

Reported nucleotide change:

G94A

Amino acid change:

Reported amino acid change:

E?K

Comment:

The mutation is reported to be a change from G to A at base 94. Assiming that refers to base 94 of of the CDS, it was mapped to codon E32.

(Brace et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage | maternal effect

(Brace et al., 2014)

abnormal neuroanatomy | larval stage | maternal effect (with Df(1)ED6443)

(Brace et al., 2014)

abnormal neuroanatomy | larval stage | maternal effect (with SkpA¹)

(Brace et al., 2014)

abnormal neuroanatomy | larval stage | maternal effect (with SkpA^G0389)

(Brace et al., 2014)

viable

(Brace et al., 2014)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage | maternal effect

(Brace et al., 2014)

NMJ bouton | larval stage | maternal effect

(Brace et al., 2014)

NMJ bouton | larval stage | maternal effect (with Df(1)ED6443)

(Brace et al., 2014)

NMJ bouton | larval stage | maternal effect (with SkpA¹)

(Brace et al., 2014)

NMJ bouton | larval stage | maternal effect (with SkpA^G0389)

(Brace et al., 2014)

Detailed Description

Statement

Reference

Homozygous larvae derived from homozygous females show a significant increase in the number of boutons and number of synaptic branches at the neuromuscular junction compared to wild type, while the size of the boutons is significantly reduced. Heterozygous larvae derived from homozygous females also show a significant increase in the number of boutons at the neuromuscular junction compared to wild type, although this overgrowth phenotype is less severe than that seen for homozygous larvae derived from homozygous females.

Mutant larvae show a decrease in axonal degeneration after crush injury compared to wild type.

(Brace et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SkpA^GD65 has abnormal neuroanatomy | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/Jra^Jbz.UAS

(Brace et al., 2014)

SkpA^GD65 has abnormal neuroanatomy | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Brace et al., 2014)

SkpA^GD65 has abnormal neuroanatomy | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Brace et al., 2014)

Scer\GAL4^elav.PU, SkpA^GD65, faf^UAS.cUa has lethal | dominant phenotype, suppressible | partially by wnd¹/wnd²

(Brace et al., 2014)

SkpA^GD65 has abnormal neurophysiology | larval stage phenotype, suppressible | partially by wnd¹/wnd²

(Brace et al., 2014)

Enhancer of

Statement

Reference

SkpA^GD65 is an enhancer of abnormal neuroanatomy | larval stage phenotype of hiw^ND51

(Brace et al., 2014)

Suppressor of

Statement

Reference

SkpA^GD65 is a suppressor of abnormal neuroanatomy | larval stage phenotype of α-Spec^KK101541, Scer\GAL4^elav.PU

(Brace et al., 2014)

Other

Statement

Reference

Scer\GAL4^elav.PU, SkpA^GD65, faf^UAS.cUa has lethal | dominant phenotype

(Brace et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

SkpA^GD65 has NMJ bouton | maternal effect | larval stage phenotype, suppressible | partially by wnd¹/wnd²

(Brace et al., 2014)

SkpA^GD65 has embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype, suppressible | partially by wnd¹/wnd²

(Brace et al., 2014)

SkpA^GD65 has NMJ bouton | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/Jra^Jbz.UAS

(Brace et al., 2014)

SkpA^GD65 has embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/Jra^Jbz.UAS

(Brace et al., 2014)

SkpA^GD65 has NMJ bouton | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Brace et al., 2014)

SkpA^GD65 has embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Brace et al., 2014)

SkpA^GD65 has NMJ bouton | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Brace et al., 2014)

SkpA^GD65 has embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Brace et al., 2014)

Enhancer of

Statement

Reference

SkpA^GD65 is an enhancer of NMJ bouton | larval stage phenotype of hiw^ND51

(Brace et al., 2014)

SkpA^GD65 is an enhancer of embryonic/larval neuromuscular junction | larval stage phenotype of hiw^ND51

(Brace et al., 2014)

Suppressor of

Statement

Reference

SkpA^GD65 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of α-Spec^KK101541, Scer\GAL4^elav.PU

(Brace et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in hiw^ND51 mutants is dramatically enhanced in hiw^ND51 SkpA^GD65 double mutants.

hiw^ND51 SkpA^GD65 double mutant larvae show a decrease in excitatory junctional potential amplitude and in quantal content compared to wild type.

The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in larvae derived from homozygous SkpA^GD65 females is significantly (but not completely) suppressed by wnd¹/wnd².

The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in larvae derived from homozygous SkpA^GD65 females is partially suppressed by expression of one of either Jra^Jbz.Scer\UAS, kay^Fbz.Scer\UAS or bsk^{K53R.Scer\UAS} under the control of Scer\GAL4^elav.PU.

The synaptic retraction phenotype seen at the neuromuscular junction in larvae expressing α-Spec^KK101541 under the control of Scer\GAL4^elav.PU is suppressed by SkpA^GD65.

The reduced axonal degeneration seen in SkpA^GD65 larvae compared to controls after crush injury is suppressed by expression of Nmnat^JF03337 under the control of Scer\GAL4^tey-5053A.

(Brace et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

SkpA^GD65/SkpA¹ is rescued by Scer\GAL4^elav.PU/SkpA^{UAS.Tag:FLAG,Tag:HA}

(Brace et al., 2014)

Not rescued by

SkpA^GD65/SkpA¹ is not rescued by SkpA^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^G7

(Brace et al., 2014)

Comments

The neuromuscular junction overgrowth phenotype (increase in number of boutons) seen in larvae derived from SkpA^GD65/SkpA¹ females is completely rescued by expression of SkpA^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^elav.PU, while expression under the control of Scer\GAL4^G7 does not rescue the phenotype.

(Brace et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GD65

(Brace et al., 2014)

SkpA^GD65

(Brace et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

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