FlyBase Allele Report: Dmel\wnd[2]

General Information

Symbol

Dmel\wnd²

Species

D. melanogaster

Name

FlyBase ID

FBal0194610

Feature type

allele

Associated gene

Dmel\wnd

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Collins et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: term978N.

(Collins et al., 2006)

Mutation in the wild-type stop codon that adds an extra 104 residues to the protein.

(Collins et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3L:19,625,548..19,625,550 [-]

Reported amino acid change:

term978N

Comment:

The reported stop to Asn amino acid change (TAA to AAT/C) requires substitution at two positions in the codon.

(Collins et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by shot³, tau^MR22

(Voelzmann et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | conditional (with wnd¹)

(Xiong et al., 2010)

abnormal neuroanatomy | larval stage (with wnd¹)

(Klinedinst et al., 2013)

Phenotype Manifest In

larval segmental nerve | conditional (with wnd¹)

(Xiong et al., 2010)

NMJ bouton | larval stage (with wnd¹)

(Klinedinst et al., 2013)

Detailed Description

Statement

Reference

wnd¹/wnd² larvae show an increase in maximum bouton diameter at the neuromuscular junction compared to controls.

The ability of injured axons to sprout after injury is inhibited in wnd¹/wnd² larvae.

(Klinedinst et al., 2013)

Under control conditions, wnd²/wnd³ transheterozygous third instar larvae do not display significant changes in the number of sensory axons per nerve, as compared to controls.

(Bhattacharya et al., 2012)

Axonal sprouting of the injured axon after a crush injury to the segmental nerve in third instar larvae is dramatically inhibited in wnd¹/wnd² animals.

(Xiong et al., 2010)

wnd² mutants exhibit wild-type synapse size.

(Franciscovich et al., 2008)

wnd¹/wnd² mutants exhibit normal synaptic function.

(Collins et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wnd¹/wnd² is a suppressor | partially of lethal | dominant phenotype of Scer\GAL4^elav.PU, SkpA^GD65, faf^UAS.cUa

(Brace et al., 2014)

wnd¹/wnd² is a suppressor | partially of abnormal neurophysiology | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd[+]/wnd² is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of shot^VV/shot³

(Valakh et al., 2013)

wnd³/wnd² is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of shot^VV/shot³

(Valakh et al., 2013)

wnd[+]/wnd² is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, sgg^A81T.UAS

(Franciscovich et al., 2008)

wnd¹/wnd² is a suppressor of abnormal neurophysiology phenotype of hiw^ND8

(Collins et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

wnd¹/wnd² is a suppressor | partially of NMJ bouton | maternal effect | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd¹/wnd² is a suppressor | partially of embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd[+]/wnd² is a suppressor | partially of NMJ bouton | increased number | third instar larval stage phenotype of shot^VV/shot³

(Valakh et al., 2013)

wnd³/wnd² is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of shot^VV/shot³

(Valakh et al., 2013)

wnd[+]/wnd² is a suppressor of synapse phenotype of Scer\GAL4^elav-C155, sgg^A81T.UAS

(Franciscovich et al., 2008)

wnd¹/wnd² is a suppressor of neuromuscular junction phenotype of Scer\GAL4^elav-C155, faf^EP381

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of bouton phenotype of Scer\GAL4^elav-C155, faf^EP381

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of neuromuscular junction phenotype of hiw^ND8

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of bouton phenotype of hiw^ND8

(Collins et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in larvae derived from homozygous SkpA^GD65 females is significantly (but not completely) suppressed by wnd¹/wnd².

(Brace et al., 2014)

wnd²/+ partially suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

wnd²/wnd³ fully suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

A wnd²/+ background completely abolishes the synapse growth seen through sgg^{A81T.Scer\UAS} expression pan-neuronally under the control of Scer\GAL4^elav-C155.

(Franciscovich et al., 2008)

hiw^ND8; wnd¹/wnd² mutants are not significantly different from wild-type, indicating complete suppression of hiw^ND8.

wnd¹/wnd² mutants do not suppress the hiw^ND8 defect in quantal size: both the amplitude and the frequency of spontaneous miniature events in the hiw^ND8; wnd¹/wnd² double mutant are similar to wild-type. In contrast, the evoked potentials of the double mutant are only modestly increased, and this is due entirely to the increased quantal size. The quantal content, calculated as the excitatory junction potential (EJP) amplitude divided by the miniature EJP (mEJP) remains the same between hiw^ND8 and hiw^ND8; wnd¹/wnd² double mutants. Therefore wnd does not suppress the primary defect in hiw^ND8 synaptic function, the reduced number of vesicles released by the nerve.

(Collins et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wnd²

(Wolfstetter et al., 2020, Voelzmann et al., 2016, Brace et al., 2014, Kim et al., 2013, Klinedinst et al., 2013, Valakh et al., 2013, Bhattacharya et al., 2012, Nechipurenko and Broihier, 2012, Xiong et al., 2010, Shen and Ganetzky, 2009, Franciscovich et al., 2008, Collins et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (12)

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