FlyBase Allele Report: Dmel\shot[VV]

General Information

Symbol

Dmel\shot^VV

Species

D. melanogaster

Name

FlyBase ID

FBal0294003

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Valakh et al., 2013)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Valakh et al., 2013)

abnormal neuroanatomy | third instar larval stage (with shot³)

(Valakh et al., 2013)

Phenotype Manifest In

NMJ bouton | increased number | third instar larval stage

(Valakh et al., 2013)

NMJ bouton | increased number | third instar larval stage (with shot³)

(Valakh et al., 2013)

Detailed Description

Statement

Reference

Homozygous and shot³/shot^VV third instar larvae show overgrowth (increase in bouton number) at the neuromuscular junction.

shot³/shot^VV axons show enhanced regenerative axonal sprouting compared to controls after a crushing injury.

(Valakh et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by wnd[+]/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wnd³/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Valakh et al., 2013)

shot^VV/shot³ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Valakh et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible | partially by wnd[+]/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by wnd³/wnd²

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/kay^Fbz.UAS

(Valakh et al., 2013)

shot^VV/shot³ has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PU/bsk^K53R.UAS

(Valakh et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

wnd²/+ partially suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

wnd²/wnd³ fully suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

Expression of either kay^Fbz.Scer\UAS or bsk^{K53R.Scer\UAS} under the control of Scer\GAL4^elav.PU suppresses the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

shot³

(Valakh et al., 2013)

Rescued by

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^LA.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^{LA-ΔPlakin.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is rescued by Scer\GAL4^elav.PU/shot^{LA-Δrod1.UAS.GFP}

(Valakh et al., 2013)

Not rescued by

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^LA.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^LC.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^LC.UAS.GFP

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^{LA-ΔEF-hand.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-ΔEF-hand.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by shot^{LA-ΔPlakin.UAS.GFP}/Scer\GAL4^how-24B

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-Δrod1.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^elav.PU/shot^{LA-ΔGAS2.UAS.GFP}

(Valakh et al., 2013)

shot^VV/shot³ is not rescued by Scer\GAL4^how-24B/shot^{LA-ΔGAS2.UAS.GFP}

(Valakh et al., 2013)

Comments

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae, while expression under the control of Scer\GAL4^how-24B fails to rescue the phenotype.

Expression of either shot^{LA-ΔPlakin.Scer\UAS.T:Avic\GFP} or shot^{LA-Δrod1.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

Expression of either shot^{LC.Scer\UAS.T:Avic\GFP}, shot^{LA-ΔEF-hand.Scer\UAS.T:Avic\GFP} or shot^{LA-ΔGAS2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU fails to rescue the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae.

(Valakh et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

shot^VV

(Valakh et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close