FB2024_03 , released June 25, 2024
Allele: Dmel\shotLA-ΔPlakin.UAS.GFP
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General Information
Symbol
Dmel\shotLA-ΔPlakin.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0240657
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
P{UAS-shot.L(A)-ΔPlakin-GFP}
Key Links
Transgenic product class
inframe_deletion
(Bottenberg et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Bottenberg et al., 2009)
Mutagen
in vitro construct
(Bottenberg et al., 2009)
Progenitor genotype
shotLA.UAS.GFP
(Bottenberg et al., 2009)
Carried in construct
P{UAS-shot.L(A)-ΔPlakin-GFP}
Cytology
Description

Deletion of sequences encoding amino acid residues 488-1325 corresponding to the Plakin-like domain of a shot L(A) isoform.

(Bottenberg et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav.PU/shotLA-ΔPlakin.UAS.GFP rescues shotVV/shot3

      (Valakh et al., 2013)
      Partially rescues

      shotLA-ΔPlakin.UAS.GFP/Scer\GAL4unspecified partially rescues shotunspecified

      (Bottenberg et al., 2009)

      shotLA-ΔPlakin.UAS.GFP/Scer\GAL4Vsx1-MzVum partially rescues shotunspecified

      (Bottenberg et al., 2009)
      Fails to rescue

      shotLA-ΔPlakin.UAS.GFP/Scer\GAL4how-24B fails to rescue shotVV/shot3

      (Valakh et al., 2013)
      Comments

      Expression of shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.

      (Valakh et al., 2013)

      shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP expression in tendon cells fully rescues the shotunspecified mutant stretch phenotype. While shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP expression fully rescues the shotunspecified mutant dendritic growth phenotypes, targeted expression of shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP in shotunspecified mutant motorneurons achieves only a partial rescue (47%) of the motoraxonal stall phenotype.

      Scer\GAL4Vsx1-MzVum driven expression of shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP produces a partial rescue of axonal extension (67%) and a full rescue of dendritic growth.

      (Bottenberg et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shotLA-ΔPlakin.Scer\UAS.T:Avic\GFP
      shotLA-ΔPlakin.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)