FLP mediated recombination between the two progenitor insertions, PBac{RB}Cp110e00667 and P{XP}l(1)G0196d05025 has deleted the Cp110 coding region.
Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them, removing the Cp110 coding sequence.
Cp110Δ mutant males display long centriole phenotype in some male germline stem cells in adult testes.
There is no difference in centriole number or number of mitotic defects between Cp110Δ mutant larval brain cells and wild-type.
Cp110Δ syncytial embryos do not exhibit any defects in centrosome behavior, spindle formation, progression through the cell cycle, or chromosome segregation.
Cp110Δ primary spermatocytes exhibit centrioles that are indistinguishable from wild-type. Older spermatocytes develop centrioles that may be slightly elongated and axonemes that are slightly shorter than in wild-type. Approximately 4% of axonemes exhibit one or more missing microtubule doublet. The centrioles in Cp110Δ spermatocytes also display a slight but significant tendency to separate prematurely (often leading to centriole mis-segregation during meiosis).
Cp110Δ mutant larval wing disc centrioles exhibit extensive microtubule protrusions emanating from their distal ends. The protrusions are often many times longer than the centrioles themselves - the longest being >5υm in length, which is approximately 50 times longer than the centriole. These microtubule protrusions are also seen in larval brain cells.
The centrioles in Cp110Δ wing discs are slightly (approximately 10%) but significantly longer than those in wild-type controls.
Cp110Δ has abnormal locomotor behavior phenotype, enhanceable by Cep97Δ
Cp110Δ has abnormal touch response | third instar larval stage phenotype, non-enhanceable by Cep97Δ
Cp110Δ has male semi-sterile phenotype, non-enhanceable by Cep97Δ
Cp110Δ has decreased fecundity | male phenotype, non-enhanceable by Cep97Δ
Cp110Δ has abnormal touch response | third instar larval stage phenotype, non-suppressible by Cep97Δ
Cp110Δ has male semi-sterile phenotype, non-suppressible by Cep97Δ
Cp110Δ has decreased fecundity | male phenotype, non-suppressible by Cep97Δ
Cp110Δ is a non-enhancer of male semi-sterile phenotype of Cep97Δ
Cp110Δ is a non-enhancer of decreased fecundity | male phenotype of Cep97Δ
Cp110Δ is a non-enhancer of abnormal touch response | third instar larval stage phenotype of Cep97Δ
Cp110Δ is a non-enhancer of abnormal locomotor behavior phenotype of Cep97Δ
Cp110Δ is a non-suppressor of abnormal touch response | third instar larval stage phenotype of Cep97Δ
Cp110Δ is a non-suppressor of abnormal locomotor behavior phenotype of Cep97Δ
Cp110Δ is a non-suppressor of male semi-sterile phenotype of Cep97Δ
Cp110Δ is a non-suppressor of decreased fecundity | male phenotype of Cep97Δ
Cp110Δ has spermatozoon phenotype, enhanceable by Cep97Δ
Cp110Δ has centriole phenotype, enhanceable by Sas-4Ubi.P.GFP
Cp110Δ has wing disc | third instar larval stage phenotype, non-enhanceable by Cep97Δ
Cp110Δ has wing disc | third instar larval stage phenotype, non-suppressible by Cep97Δ
Cp110Δ is a non-enhancer of primary spermatocyte | P-stage phenotype of Cep97Δ
Cp110Δ is a non-enhancer of wing disc | third instar larval stage phenotype of Cep97Δ
Cp110Δ is a non-enhancer of spermatozoon phenotype of Cep97Δ
Cp110Δ is a non-suppressor of wing disc | third instar larval stage phenotype of Cep97Δ
Cp110Δ is a non-suppressor of spermatozoon phenotype of Cep97Δ
Cp110Δ is a non-suppressor of primary spermatocyte | P-stage phenotype of Cep97Δ
In a Cp110Δ background, Sas-4Ubi.P.T:Avic\GFP expression leads to an increase in centriole length by approximately 30%.
In a Cp110Δ background, Sas-4Ubi.P.T:Avic\GFP expression leads to an increase in centriole length by approximately 30%.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Scer\UAS.P\T.T:Avic\GFP does not perturb centriole length in wild-type wing disc cells.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Scer\UAS.P\T.T:Avic\GFP dramatically increases centriole length (by approximately 60% in Cp110Δ wing disc cells.
Overexpression of ana2Scer\UAS.P\T.T:Avic\GFP increases centriole length (by approximately 60% in Cp110Δ wing disc cells.
Overexpression of Sas-6Ubi.P.T:Avic\GFP increases centriole length (by approximately 60% in Cp110Δ wing disc cells.
Overexpression of Sas-6Ubi.P.T:Avic\GFP in Cp110Δ third instar larval brain cells leads to significant centriole overduplication (with approximately 60% of cells exhibiting overduplication).
Cp110Δ is rescued by Cp110S.Ubi-p63E.mGFP6
Cp110Δ is rescued by Cp110L.Ubi-p63E.mGFP6
A Cp110S.Ubi-p63E.T:Avic\GFP-m6 background rescues premature centriole mis-segregation during meiosis in Cp110Δ mutants.
The formation of microtubule protrusions from Cp110Δ centrioles is suppressed by the presence of Cp110S.Ubi-p63E.T:Avic\GFP-m6.
A Cp110L.Ubi-p63E.T:Avic\GFP-m6 background rescues premature centriole mis-segregation during meiosis in Cp110Δ mutants.
The formation of microtubule protrusions from Cp110Δ centrioles is suppressed by the presence of Cp110L.Ubi-p63E.T:Avic\GFP-m6.
Expression of Cp110L.Ubi-p63E.T:Avic\GFP-m6 rescues the long centriole phenotype found in Cp110Δ wing disc cells.
Expression of Cp110L.Ubi-p63E.T:Avic\GFP-m6 weakly rescues the long centriole phenotype found in Cp110Δ wing disc cells.