FB2024_03 , released June 25, 2024
Allele: Dmel\Sas-4Ubi.P.GFP
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General Information
Symbol
Dmel\Sas-4Ubi.P.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0240464
Feature type
allele
Associated gene
Dmel\Sas-4
Associated Insertion(s)
Carried in Construct
P{Ubi-Sas-4-GFP.P}
Also Known As
DSas-4-GFP, Ubq-DSas-4-GFP, GFP-SAS-4, Sas4-GFP
Key Links
Transgenic product class
wild_type
(Peel et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Peel et al., 2007)
Mutagen
in vitro construct
(Peel et al., 2007)
Progenitor genotype
Carried in construct
P{Ubi-Sas-4-GFP.P}
Cytology
Description

A ubiquitin promoter drives expression of Sas-4 which is tagged with GFP.

(Peel et al., 2007)
Allele components
Component
Use(s)
Encoded product / tool
Sas-4
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Sas-4 by approximately fivefold in wild-type wing disc cells (through expression of Sas-4Ubi.P.T:Avic\GFP) does not increase centriole length nor detectable centriole microtubule protrusions.

      The overexpression of Sas-4Ubi.P.T:Avic\GFP does not cause centriole overduplication in spermatocytes or syncytial embryos.

      (Franz et al., 2013)

      Centrioles replicate normally in syncytial embryos expressing Sas-4Ubi.P.T:Avic\GFP. No extra centrioles are detected in the female germline, male germline or mitotic third larval instar brain cells in animals expressing Sas-4Ubi.P.T:Avic\GFP.

      Expression of Sas-4Ubi.P.T:Avic\GFP does not result in de novo formation of centriole-like structures in unfertilised eggs (which do not normally contain centrioles).

      (Peel et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Sas-4Ubi.P.GFP is an enhancer of centriole phenotype of Cp110Δ

      (Franz et al., 2013)
      NOT Enhancer of
      Statement
      Reference

      Sas-4Ubi.P.GFP is a non-enhancer of centriole phenotype of ana2Ubi.C.GFP

      (Stevens et al., 2010)

      Sas-4Ubi.P.GFP is a non-enhancer of centriole phenotype of Sas-6Ubi.P.GFP

      (Stevens et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In a Cp110Δ background, Sas-4Ubi.P.T:Avic\GFP expression leads to an increase in centriole length by approximately 30%.

      The overexpression of Sas-4Ubi.P.T:Avic\GFP in Cp110Δ mutant syncytial embryos does not cause centriole overduplication.

      (Franz et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sas-4Ubi.P.GFP
      Sas-4Ubi.P.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (17)