Expression of Sas-6Ubi.P.GFP does not lead to the formation of the procentriolar ectopic particles in mature primary spermatocytes when compared to controls.
Overexpression of Sas-6Ubi.P.T:Avic\GFP leads to some centriole overduplication in third instar larval brain cells.
The overexpression of Sas-6Ubi.P.T:Avic\GFP does not cause centriole overduplication in spermatocytes.
Overexpression of Sas-6Ubi.P.T:Avic\GFP in syncytial embryos results causes approximately 18% of centrioles to overduplicate.
Unfertilized eggs laid by mothers carrying two copies of sas-6Ubi.P.T:Avic\GFP assemble centriole-like structures.
Approximately 3% of centrioles proceed through an extra round of template-driven replication within a single nuclear cycle in syncytial embryos expressing sas-6Ubi.P.T:Avic\GFP, and a few centrioles undergo two extra rounds of replication.
Extra centrioles are detected in mitotic third larval instar brain cells in animals expressing sas-6Ubi.P.T:Avic\GFP. The brain cells do not have dramatic mitotic defects, and the accumulation of extra centrioles is not due to a failure in cytokinesis.
No extra centrioles are detected in the female or male germline in animals expressing sas-6Ubi.P.T:Avic\GFP.
Expression of sas-6Ubi.P.T:Avic\GFP results in de novo formation of centriole-like structures in approximately 30% of unfertilised eggs (which do not normally contain centrioles).
Sas-6Ubi.P.GFP has centriole phenotype, non-enhanceable by SAKUbi.P.GFP
Sas-6Ubi.P.GFP has centriole phenotype, non-enhanceable by Sas-4Ubi.P.GFP
Sas-6Ubi.P.GFP, ana2Ubi.C.GFP has primary spermatocyte phenotype
Poc1UAS.B.GFP, Sas-6Ubi.P.GFP, Scer\GAL4VP16.bam has primary spermatocyte phenotype
Expression of ana2Ubi.C.GFP in combination with Sas-6Ubi.P.GFP leads to the formation of the procentriolar ectopic particles that are located in the cytoplasm of mature and immature primary spermatocytes, and attached to the centrioles only in mature primary spermatocytes when compared to controls. Expression of Poc1UAS.B.GFP (under the control of Scer\GAL4VP16.bam) together with Sas-6Ubi.P.GFP but not with Poc1UAS.A.GFP or Poc1BΔC.UAS.GFP leads to the formation of the procentriolar ectopic particles in mature primary spermatocytes when compared to controls. Procentriolar ectopic particles in mature primary spermatocytes resemble the proximal centriole-like ultrastructure with tubule-like structures (significantly wider in Poc1 Sas-6 than Ana2 Sas-6 co-expression), spokes (only in Ana2 Sas-6 co-expression), and electron-dense material (similar diameter in both Poc1 Sas-6 than Ana2 Sas-6 co-expression).
Expression of ana2Ubi.C.GFP in combination with Sas-6Ubi.P.GFP leads to formation of procentriolar ectopic particles while their expression in Poc1W87X homozygous background leads to structural defects in procentriolar ectopic particles such as fragmented tubule-like structures and electron-dense material in mature primary spermatocytes.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Scer\UAS.P\T.T:Avic\GFP does not perturb centriole length in wild-type wing disc cells.
Co-expression of Sas-6Ubi.P.T:Avic\GFP and ana2Scer\UAS.P\T.T:Avic\GFP dramatically increases centriole length (by approximately 60% in Cp110Δ wing disc cells.
Overexpression of Sas-6Ubi.P.T:Avic\GFP increases centriole length (by approximately 60% in Cp110Δ wing disc cells.
Overexpression of Sas-6Ubi.P.T:Avic\GFP in Cp110Δ third instar larval brain cells leads to some centriole overduplication, as seen in Sas-6Ubi.P.T:Avic\GFP single mutants.
Overexpression of Sas-6Ubi.P.T:Avic\GFP in Cp110Δ mutant spermatocytes drives centriole overduplication and leads to the formation of detached extra centrioles and rosette-like structures.
Overexpression of Sas-6Ubi.P.T:Avic\GFP in Cp110Δ syncytial embryos results causes approximately 37% of centrioles to overduplicate.
Almost all unfertilized eggs laid by mothers carrying one copy of sas-6Ubi.P.T:Avic\GFP and ana2Ubi.C.T:Avic\GFP contain hundreds of large structures that stain for centriole markers, recruited PCM, and nucleated asters. These centriole-like structures often appear ring-shaped, and only one structure is contained within each aster. Eggs laid by mothers carrying one copy of either ana2Ubi.C.T:Avic\GFP or sas-6Ubi.P.T:Avic\GFP together with one copy of either SAKUbi.P.T:Avic\GFP or Sas-4Ubi.P.T:Avic\GFP exhibit at most a very small number of asters in very few eggs.
Sas-6Ubi.P.GFP rescues Sas-6c02901
The uncoordinated phenotype of sas-6c02901 adults is rescued by sas-6Ubi.P.T:Avic\GFP.
