Overexpression of Sas-6Ubi.P.T:Avic\GFP leads to significant centriole overduplication in third instar larval brain cells.
The overexpression of SAKUbi.P.T:Avic\GFP does not cause centriole overduplication in spermatocytes.
60-80% of embryos expressing SAKUbi.P.T:Avic\GFP arrest early in development with mitotic defects.
There is a dramatic increase in centriole numbers in mitotic third larval instar brain cells in animals expressing SAKUbi.P.T:Avic\GFP. The brain cells do not have dramatic mitotic defects, and the accumulation of extra centrioles is not due to a failure in cytokinesis.
No extra centrioles are detected in the female or male germline in animals expressing SAKUbi.P.T:Avic\GFP.
Expression of SAKUbi.P.T:Avic\GFP does not result in de novo formation of centriole-like structures in unfertilised eggs (which do not normally contain centrioles).
SAKUbi.P.GFP is a non-enhancer of centriole phenotype of ana2Ubi.C.GFP
SAKUbi.P.GFP is a non-enhancer of centriole phenotype of Sas-6Ubi.P.GFP
Overexpression of Sas-6Ubi.P.T:Avic\GFP in Cp110Δ third instar larval brain cells leads to significant centriole overduplication (with approximately 60% of cells exhibiting overduplication).
Overexpression of either Cp110S.Ubi-p63E.T:Avic\GFP-m6 or Cp110L.Ubi-p63E.T:Avic\GFP-m6 significantly reduces the centriole overduplication seen in third instar larval brain cells expressing SAKUbi.P.T:Avic\GFP.
Overexpression of SAKUbi.P.T:Avic\GFP in Cp110Δ mutant spermatocytes drives centriole overduplication and leads predominantly to the formation of detached extra centrioles.
Eggs laid by mothers carrying one copy of either ana2Ubi.C.T:Avic\GFP or sas-6Ubi.P.T:Avic\GFP together with one copy of either SAKUbi.P.T:Avic\GFP or Sas-4Ubi.P.T:Avic\GFP exhibit at most a very small number of asters in very few eggs.
