FlyBase Allele Report: Dmel\Cp110[Δ]

General Information

Symbol

Dmel\Cp110^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0294119

Feature type

allele

Associated gene

Dmel\Cp110

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Franz et al., 2013)

Mutagen

FLPase

(Franz et al., 2013)

Progenitor genotype

PBac{RB}Cp110^e00667

(Franz et al., 2013)

P{XP}l(1)G0196^d05025

(Franz et al., 2013)

Cytology

Description

FLP mediated recombination between the two progenitor insertions, PBac{RB}Cp110^e00667 and P{XP}l(1)G0196^d05025 has deleted the Cp110 coding region.

(Franz et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:22,482,639..22,487,424 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them, removing the Cp110 coding sequence.

(Franz et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior

(Dobbelaere et al., 2020)

abnormal touch response | third instar larval stage

(Dobbelaere et al., 2020)

decreased fecundity | male

(Dobbelaere et al., 2020)

fertile

(Franz et al., 2013)

male semi-sterile

(Dobbelaere et al., 2020)

viable

(Franz et al., 2013)

visible

(Dobbelaere et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cp110^Δ wing disc centrioles are shorter with prominent extensions of individual microtubules emanating from one end.

Cp110^Δ males exhibit a reduction in sperm motility.

Cp110^Δ adults exhibit a reduction in climbing ability and abnormal wing posture.

(Dobbelaere et al., 2020)

Cp110^Δ mutant males display long centriole phenotype in some male germline stem cells in adult testes.

(Galletta et al., 2016)

There is no difference in centriole number or number of mitotic defects between Cp110^Δ mutant larval brain cells and wild-type.

Cp110^Δ syncytial embryos do not exhibit any defects in centrosome behavior, spindle formation, progression through the cell cycle, or chromosome segregation.

Cp110^Δ primary spermatocytes exhibit centrioles that are indistinguishable from wild-type. Older spermatocytes develop centrioles that may be slightly elongated and axonemes that are slightly shorter than in wild-type. Approximately 4% of axonemes exhibit one or more missing microtubule doublet. The centrioles in Cp110^Δ spermatocytes also display a slight but significant tendency to separate prematurely (often leading to centriole mis-segregation during meiosis).

Cp110^Δ mutant larval wing disc centrioles exhibit extensive microtubule protrusions emanating from their distal ends. The protrusions are often many times longer than the centrioles themselves - the longest being >5υm in length, which is approximately 50 times longer than the centriole. These microtubule protrusions are also seen in larval brain cells.

The centrioles in Cp110^Δ wing discs are slightly (approximately 10%) but significantly longer than those in wild-type controls.

(Franz et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cp110^Δ has abnormal locomotor behavior phenotype, enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

NOT Enhanced by

Statement

Reference

Cp110^Δ has abnormal touch response | third instar larval stage phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has male semi-sterile phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has decreased fecundity | male phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

NOT suppressed by

Statement

Reference

Cp110^Δ has abnormal touch response | third instar larval stage phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has male semi-sterile phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has decreased fecundity | male phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

NOT Enhancer of

Statement

Reference

Cp110^Δ is a non-enhancer of male semi-sterile phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of decreased fecundity | male phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of abnormal touch response | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of abnormal locomotor behavior phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

NOT Suppressor of

Statement

Reference

Cp110^Δ is a non-suppressor of abnormal touch response | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of abnormal locomotor behavior phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of male semi-sterile phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of decreased fecundity | male phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cp110^Δ has spermatozoon phenotype, enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has centriole phenotype, enhanceable by Sas-4^Ubi.P.GFP

(Franz et al., 2013)

NOT Enhanced by

Statement

Reference

Cp110^Δ has centriole phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has wing disc | third instar larval stage phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

NOT suppressed by

Statement

Reference

Cp110^Δ has centriole phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ has wing disc | third instar larval stage phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

NOT Enhancer of

Statement

Reference

Cp110^Δ is a non-enhancer of primary spermatocyte | P-stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of centriole phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of wing disc | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-enhancer of spermatozoon phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

NOT Suppressor of

Statement

Reference

Cp110^Δ is a non-suppressor of centriole phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of wing disc | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of spermatozoon phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Cp110^Δ is a non-suppressor of primary spermatocyte | P-stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

In a Cp110^Δ background, Sas-4^{Ubi.P.T:Avic\GFP} expression leads to an increase in centriole length by approximately 30%.

Co-expression of Sas-6^{Ubi.P.T:Avic\GFP} and ana2^{Scer\UAS.P\T.T:Avic\GFP} does not perturb centriole length in wild-type wing disc cells.

Co-expression of Sas-6^{Ubi.P.T:Avic\GFP} and ana2^{Scer\UAS.P\T.T:Avic\GFP} dramatically increases centriole length (by approximately 60% in Cp110^Δ wing disc cells.

Overexpression of ana2^{Scer\UAS.P\T.T:Avic\GFP} increases centriole length (by approximately 60% in Cp110^Δ wing disc cells.

Overexpression of Sas-6^{Ubi.P.T:Avic\GFP} increases centriole length (by approximately 60% in Cp110^Δ wing disc cells.

Overexpression of Sas-6^{Ubi.P.T:Avic\GFP} in Cp110^Δ third instar larval brain cells leads to significant centriole overduplication (with approximately 60% of cells exhibiting overduplication).

(Franz et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cp110^Δ is rescued by Cp110^{S.Ubi-p63E.mGFP6}

(Franz et al., 2013)

Cp110^Δ is rescued by Cp110^{L.Ubi-p63E.mGFP6}

(Franz et al., 2013)

Comments

A Cp110^{S.Ubi-p63E.T:Avic\GFP-m6} background rescues premature centriole mis-segregation during meiosis in Cp110^Δ mutants.

The formation of microtubule protrusions from Cp110^Δ centrioles is suppressed by the presence of Cp110^{S.Ubi-p63E.T:Avic\GFP-m6}.

A Cp110^{L.Ubi-p63E.T:Avic\GFP-m6} background rescues premature centriole mis-segregation during meiosis in Cp110^Δ mutants.

The formation of microtubule protrusions from Cp110^Δ centrioles is suppressed by the presence of Cp110^{L.Ubi-p63E.T:Avic\GFP-m6}.

Expression of Cp110^{L.Ubi-p63E.T:Avic\GFP-m6} rescues the long centriole phenotype found in Cp110^Δ wing disc cells.

Expression of Cp110^{L.Ubi-p63E.T:Avic\GFP-m6} weakly rescues the long centriole phenotype found in Cp110^Δ wing disc cells.

(Franz et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CP110^Δ

(Dobbelaere et al., 2020)

CP110Δ

(Aydogan et al., 2022)

Cp110^Δ

cp110^Δ

(Galletta et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (4)

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