FlyBase Allele Report: Dmel\Rpp30[18.2]

General Information

Symbol

Dmel\Rpp30^18.2

Species

D. melanogaster

Name

FlyBase ID

FBal0287883

Feature type

allele

Associated gene

Dmel\Rpp30

Associated Insertion(s)

Carried in Construct

Also Known As

18.2

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Molla-Herman et al., 2015, Jagut et al., 2013)

hypomorphic allele - molecular evidence

(Molla-Herman et al., 2015)

Mutagen

ethyl methanesulfonate

(Jagut et al., 2013)

Progenitor genotype

Cytology

Description

Amino acid replacement: L83P.

(Molla-Herman et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:252,010..252,010 [-]

Nucleotide change:

T252010C

Amino acid change:

L83P | Rpp30-PB; L83P | Rpp30-PA

Reported amino acid change:

L83P

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Molla-Herman et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Jagut et al., 2013)

lethal (with Df(2L)ED21)

(Molla-Herman et al., 2015)

male sterile

(Jagut et al., 2013)

partially lethal

(Jagut et al., 2013)

partially lethal - majority die | recessive | heat sensitive

(Molla-Herman et al., 2015)

sterile (with Rpp30^k01901)

(Molla-Herman et al., 2015)

viable (with Rpp30^k01901)

(Molla-Herman et al., 2015)

Phenotype Manifest In

egg chamber

(Jagut et al., 2013)

egg chamber (with Rpp30^k01901)

(Molla-Herman et al., 2015)

egg chamber | germline clone

(Molla-Herman et al., 2015, Jagut et al., 2013)

oocyte | germline clone

(Jagut et al., 2013)

ovary

(Molla-Herman et al., 2015)

ovary (with Rpp30^k01901)

(Molla-Herman et al., 2015)

Detailed Description

Statement

Reference

10% of homozygotes survive to adulthood at 25[o]C, and 20% survive at 18[o]C. Homozygous and Rpp30^18.2/Rpp30^k01901 flies are normal in size.

Homozygous ovaries are rudimentary due to oogenesis arrest at an early stage of development. Homozygous clones in the germline result in egg chambers that arrest as early as in homozygous mutant ovaries, whereas egg chambers in which all follicle cells are homozygous develop normally.

(Molla-Herman et al., 2015)

Egg chambers containing coconut^18.2 mutant germline clones arrest oogenesis at stage 4-5. In some cases the oocyte is mislocalised within the egg chamber. Oogenesis arrests slightly earlier in homozygous coconut^18.2 mutant escapers.

(Jagut et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Rpp30^18.2 has sterile phenotype, non-suppressible by p53^11-1B-1

(Molla-Herman et al., 2015)

Other

Statement

Reference

Claspin⁴⁵, Rpp30^18.2 has lethal phenotype

(Molla-Herman et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rpp30^k01901/Rpp30^18.2 has egg chamber phenotype, suppressible | partially by Claspin^aq5

(Molla-Herman et al., 2015)

Rpp30^k01901/Rpp30^18.2 has egg chamber phenotype, suppressible | partially by Claspin²⁷⁹

(Molla-Herman et al., 2015)

Rpp30^k01901/Rpp30^18.2 has egg chamber phenotype, suppressible | partially by Claspin^aq4

(Molla-Herman et al., 2015)

Rpp30^k01901/Rpp30^18.2 has egg chamber phenotype, suppressible | partially by sqd^S.Tag:HA

(Molla-Herman et al., 2015)

Rpp30^18.2 has egg chamber phenotype, suppressible by p53^11-1B-1

(Molla-Herman et al., 2015)

Rpp30^18.2 has egg chamber phenotype, suppressible by lok^p6

(Molla-Herman et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Some egg chambers are found developing to late stages of oogenesis or even forming eggs in Rpp30^18.2 ; p53^11-1B-1 double mutant females. This rescue is partial, as the majority of egg chambers are still arrested early and the flies are still sterile.

Most egg chambers develop into eggs in Rpp30^18.2 ; lok^p6 double mutant females, and these eggs can be laid and fertilised.

(Molla-Herman et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rpp30^k01901/Rpp30^18.2 is rescued by Scer\GAL4^nanos.PU/Rpp30^UASp.GFP

(Molla-Herman et al., 2015)

Rpp30^18.2 is rescued by Rpp30^Ubi.GFP

(Molla-Herman et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

18.2

(Molla-Herman et al., 2015, Jagut et al., 2013)

Rpp30^18.2

(Molla-Herman et al., 2020)

coconut^18.2

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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