UAS regulatory sequences drive expression of a mutated form of msl-1 containing the amino acid replacements V114D, M121D, M142D, V145D and K131D. The protein is tagged at the C-terminal end with three copies of Tag:FLAG.
lethal | female | heat sensitive, with Scer\GAL4αTub84B.PL
lethal | male, with Scer\GAL4αTub84B.PL
Scer\GAL4αTub84B.PL/msl-1mut3.UAS.Tag:FLAG fails to rescue msl-1L60/msl-1γ269
Expression of msl-1mut3.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4αTub84B.PL at 25[o]C fails to rescue the male-specific lethality of msl-1L60/msl-1γ269 flies.