UAS regulatory sequences drive expression of a mutated form of msl-1 containing the amino acid replacements E886R, F893R, H897R, F945E, A965E and F979E. The protein is tagged at the C-terminal end with three copies of Tag:FLAG.
lethal | male limited | heat sensitive, with Scer\GAL4αTub84B.PL
msl-1mut1.UAS.Tag:FLAG/Scer\GAL4αTub84B.PL fails to rescue msl-1L60/msl-1γ269
Expression of msl-1mut1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4αTub84B.PL at 25[o]C fails to rescue the male-specific lethality of msl-1L60/msl-1γ269 flies.