FlyBase Allele Report: Dmel\lin-52[93]

General Information

Symbol

Dmel\lin-52⁹³

Species

D. melanogaster

Name

FlyBase ID

FBal0277116

Feature type

allele

Associated gene

Dmel\lin-52

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lewis et al., 2012)

Mutagen

Delta2-3 transposase

(Lewis et al., 2012)

Progenitor genotype

P{EP}EP404

(Lewis et al., 2012)

Cytology

Description

Imprecise excision of the P{EP} element in P{EP}EP404 has resulted in a 1302bp deletion that removes the entire lin-52 gene.

(Lewis et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:5,753,624..5,754,925 [-]

Comment:

1302 bp deletion resulting from the imprecise excision of P{EP}EP404.

(Lewis et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | germline clone | maternal effect

(Lewis et al., 2012)

lethal | embryonic stage | germline clone | maternal effect

(Lewis et al., 2012)

lethal | larval stage

(Lewis et al., 2012)

visible | adult stage | somatic clone

(Lewis et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Eyes composed entirely of lin-52⁹³ mutant clone cells (generated using the ey-FLP method) are rough and include small rhabdomeres and irregular ommatidia. When lin-52⁹³ mutant eye clones are generated alongside wild type control tissue mutant the mutant ommatidia are disorganised and contain rhabdomeres that are distended and/or absent compared to the adjacent control cells. No defects are observed in the control cells, indicating that the phenotype is cell-autonomous.

Less than 1% of embryos derived from females carrying homozygous lin-52⁹³ germline clones crossed to wild type males are able to hatch. in 86% of embryos mitotic arrest occurs when the embryo contains between two and eight nuclei. In many of these embryos the mitotic spindles and metaphase plate have failed to form correctly, with condensed chromosomes often distributed diffusely between the spindle poles. One or both centrosomes are often missing and other defects observed include a broad spindle with no centrosomes, a branched spindle with no centrosomes or an elongated spindle with no centrosomes.

Generation of lin-52⁹³ mutant clones in the germline or ovarian somatic cells has no effect on the morphology of these cells, nor on the progression of oogenesis.

(Lewis et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lin-52⁹³ has lethal | larval stage phenotype, suppressible by mip40^EY16520

(Lewis et al., 2012)

lin-52⁹³ has visible | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has visible | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

(Lewis et al., 2012)

lin-52⁹³ has lethal | germline clone | maternal effect | embryonic stage phenotype, suppressible | partially by mip120⁶⁷/mEFTu1^+tBa/mip120[+]

(Lewis et al., 2012)

NOT suppressed by

Statement

Reference

lin-52⁹³ has lethal | larval stage phenotype, non-suppressible by mip40[+]/mip40^EY16520

(Lewis et al., 2012)

lin-52⁹³ has visible | somatic clone | adult stage phenotype, non-suppressible by Myb^MH107

(Lewis et al., 2012)

(Lewis et al., 2012)

lin-52⁹³ has lethal | germline clone | maternal effect | embryonic stage phenotype, non-suppressible by mip40[+]/mip40^EY16520

(Lewis et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

lin-52⁹³ has rhabdomere | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

lin-52⁹³ has ommatidium | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has eye | somatic clone phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has ommatidium | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

Myb^MH107, lin-52⁹³ has rhabdomere | somatic clone | adult stage phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

(Lewis et al., 2012)

lin-52⁹³ has embryo | germline clone | maternal effect phenotype, suppressible | partially by mip120⁶⁷/mEFTu1^+tBa/mip120[+]

(Lewis et al., 2012)

lin-52⁹³ has eye | somatic clone phenotype, suppressible by mip130^1-36

(Lewis et al., 2012)

NOT suppressed by

Statement

Reference

lin-52⁹³ has eye | somatic clone phenotype, non-suppressible by Myb^MH107

(Lewis et al., 2012)

lin-52⁹³ has rhabdomere | somatic clone | adult stage phenotype, non-suppressible by Myb^MH107

(Lewis et al., 2012)

lin-52⁹³ has ommatidium | somatic clone | adult stage phenotype, non-suppressible by Myb^MH107

(Lewis et al., 2012)

lin-52⁹³ has embryo | germline clone | maternal effect phenotype, non-suppressible | germline clone | maternal effect by Myb^MH107

(Lewis et al., 2012)

lin-52⁹³ has embryo | germline clone | maternal effect phenotype, non-suppressible by mip40[+]/mip40^EY16520

(Lewis et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

mip40^EY16520 rescues the lethality seen in homozygous and hemizygous lin-52⁹³ larvae. Both male and female adult flies are seen at a similar frequency to controls. One copy of mip40^EY16520 is not sufficient to rescue the lethality.

Homozygous mip130^1-36 suppresses the eye phenotypes seen in lin-52⁹³ mutant eye clones. The mutant tissue appears completely normal.

Homozygous Myb^MH107 does not suppress the eye phenotypes seen in lin-52⁹³ mutant eye clones.

Homozygous mip130^1-36 suppresses the eye phenotypes seen in Myb^MH107, lin-52⁹³ double mutant clones. The resulting triple mutant eyes appear completely normal.

Approximately 29% of embryos derived from females carrying homozygous lin-52⁹³ mip130^1-36 double mutant germline clones crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-52⁹³ alone.

Approximately 2% of embryos derived from females carrying homozygous lin-52⁹³ Myb^MH107 double mutant germline clones crossed to wild type males are able to hatch, a similar rate to when females are maternally mutant for lin-52⁹³ alone.

Embryos derived from females that carry homozygous lin-52⁹³ mutant germline clones and are heterozygous for mip40^EY16520 crossed to wild type males are unable to hatch, similar to when females are maternally mutant for lin-52⁹³ alone.

Approximately 21% of embryos derived from females that carry homozygous lin-52⁹³ mutant germline clones and are heterozygous for mip120⁶⁷ (Df(2R)mip120⁶⁷ mutants in which EfTuM[+] function has been restored using EfTuM^+tBa) crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-52⁹³ alone.

(Lewis et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lin-52⁹³ is rescued by lin-52^+t1.6

(Lewis et al., 2012)

lin-52⁹³ is rescued by lin-52^GFP

(Lewis et al., 2012)

lin-52⁹³ is rescued by lin-52^{Ubi-p63E.Tag:FLAG}

(Lewis et al., 2012)

Comments

Expression of lin-52^+t1.6 rescues the eye phenotypes seen in eye composed entirely of lin-52⁹³ mutant somatic clone cells.

Expression of lin-52^+t1.6 rescues the embryonic lethality seen in embryos derived from females carrying homozygous lin-52⁹³ germline clones crossed to wild type males.

Expression of lin-52^T:Avic\GFP rescues the lethality seen in lin-52⁹³ mutant larvae.

Expression of lin-52^{Ubi-p63E.T:Zzzz\FLAG} rescues the lethality seen in lin-52⁹³ mutant larvae.

(Lewis et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lin-52⁹³

(Debruhl et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (2)

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