FlyBase Allele Report: Zzzz\CAG[CAA.94.UAS.Tag:MYC,Tag:FLAG]

General Information

Symbol

Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

Species

a. artificial

Name

FlyBase ID

FBal0276808

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{UAS-CAA.94}

Transgenic product class

trinucleotide_repeat_expansion

(McLeod et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(McLeod et al., 2005)

Mutagen

in vitro construct

(McLeod et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-CAA.94}

Cytology

Description

UASt regulatory sequences drive expression of a poly-glutamine-repeat encode by 94 CAA codons fused with C-terminal Tag:MYC and Tag:FLAG tags.

(McLeod et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(van Eyk et al., 2011, McLeod et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is exacerbated by mod(mdg4)^GD16547

(van Eyk et al., 2011)

is NOT exacerbated by Mef2^GD5039

(van Eyk et al., 2011)

is exacerbated by sgg^UAS.cBa

(van Eyk et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^GMR.PF

(McLeod et al., 2005)

viable, with Scer\GAL4^da.G32

(Lawlor et al., 2012)

visible, with Scer\GAL4^GMR.PF

(McLeod et al., 2005)

visible, with Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(McLeod et al., 2005)

eye, with Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

ommatidium, with Scer\GAL4^GMR.PF

(McLeod et al., 2005)

pigment cell, with Scer\GAL4^GMR.PF

(McLeod et al., 2005)

Detailed Description

Statement

Reference

The tergites of flies expressing Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^da.G32 appear normal compared to wild-type.

(Lawlor et al., 2012)

Expression of Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in pigmentation loss in the eye.

(van Eyk et al., 2011)

Expression of Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} in the developing eye under the control of Scer\GAL4^GMR.PF causes a dramatic phenotype with disorganization of the ommatidia, loss of pigment cells and a collapsed eye.

Depending on the insertion line used, expression of Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF results in phenotypes ranging in severity from wild-type to lethality.

(McLeod et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, enhanceable by sgg^UAS.cBa/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has visible phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, enhanceable by mod(mdg4)^GD16547/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, enhanceable by sgg^UAS.cBa/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, non-enhanceable by Mef2^GD5039, Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, non-enhanceable by sgg^KK108994/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, non-suppressible by Mef2^GD5039, Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Scer\GAL4^GMR.PFa, Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG} has eye phenotype, non-suppressible by sgg^KK108994/Scer\GAL4^GMR.PFa

(van Eyk et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Mef2^GD5039 with Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa does not alter the appearance of the eye.

Co-expression of mod(mdg4)^GD16547 with Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in a mild enhancement of the Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}-induced eye pigmentation defects.

Co-expression of sgg^Scer\UAS.cBa with Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa results in an increase in the eye compared with ectopic expression of sgg^Scer\UAS.cBa alone. There appears to be a reduction in the amount of pigment in the eye and in most cases there are necrotic patches and nearly complete loss of ommatidial array structure.

Co-expression of sgg^KK108994 with Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PFa does not dramatically alter the exterior appearance of the eye compared with flies expressing Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} alone.

(van Eyk et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Zzzz\CAG^{CAA.94.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

Zzzz\CAG^{CAA.94.T:Hsap\MYC,T:Zzzz\FLAG}

Zzzz\CAG^{CAA.94.UAS.Tag:MYC,Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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