FlyBase Allele Report: Dmel\Sumo[RNAi.UAS]

General Information

Symbol

Dmel\Sumo^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0276748

Feature type

allele

Associated gene

Dmel\Sumo

Associated Insertion(s)

Carried in Construct

P{UAS-Sumo.RNAi}

Also Known As

UAS-smt3i

Key Links

Transgenic product class

RNAi_reagent

(Talamillo et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Talamillo et al., 2008)

Mutagen

in vitro construct

(Talamillo et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Sumo.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a smt3 RNAi construct.

(Talamillo et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sumo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

eye, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

eye disc, with Scer\GAL4^ey.PU

(Kanakousaki and Gibson, 2012)

follicle cell, with Scer\GAL4^T155

(Talamillo et al., 2013)

lipid droplet, with Scer\GAL4^phtm.PO

(Talamillo et al., 2013, Talamillo et al., 2008)

prothoracic gland, with Scer\GAL4^phtm.PO

(Talamillo et al., 2013, Talamillo et al., 2008)

submarginal cell, with Scer\GAL4^salm.EPv

(Ureña et al., 2016)

wing, with Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Detailed Description

Statement

Reference

The expression of Sumo^dsRNA.UAS under the control of Scer\GAL4^phm.PO leads to increased growth rate in hypoxic conditions (increased larval weight in 10% O[[2]] compared to controls).

(Kapali et al., 2022)

The expression of smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^salm.EPv leads to a small but significant decrease in the total wing area, coupled with a severe area decrease of the intervein region between L2 and L3, as compared to controls; expression under the control of Scer\GAL4^phm.PO leads to a complete development arrest at the larval stage.

(Ureña et al., 2016)

The follicle cells in flies expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^T155 show a strong reduction in the number of lipid droplets. Egg fertility is only slightly affected and viable embryos are obtained.

The prothoracic gland cells in larvae expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO have a reduced lipid content.

(Talamillo et al., 2013)

Expression of smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in a mutant phenotype in the eye.

Expression of smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PU results in a dramatic reduction in the size of the eye disc and severely reduced adult eyes. In many cases, head formation is abolished and the animals do not eclose.

(Kanakousaki and Gibson, 2012)

Animals expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO arrest their development at the third larval instar just before pupariation and survive to an additional 3 weeks. During this time, the larvae continue feeding and gain weight. Mutant third instar larvae fed with medium containing 20-hydroxyecdysone pupariate within 24 hours, but do not develop further.

At 120-144 hours after egg laying (AEL), some prothoracic gland cells in larvae expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO are increased in size. The nuclei of these cells are enlarged and show abnormal morphology. From 7 to 22 days AEL in the mutant larvae, the prothoracic gland cells and their nuclei continue to grow in size, and at the same time, the number of prothoracic gland cells progressively decreases. Massive cell death or degeneration are not seen in the mutant prothoracic glands. The cells show a decrease in the number of lipid droplets per cell compared to wild type.

Ultrastructurally, the prothoracic gland cells of larvae expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO show a clear reduction in the number and length of invaginations of the plasma membrane compared to wild-type cells. The number, size and morphology of the mitochondria appear normal. The nuclear lamina appears thickened, but nuclear pores appear not to be affected.

(Talamillo et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has visible | adult stage phenotype, enhanceable by Hsap\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has abnormal size | adult stage phenotype, enhanceable by Hsap\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has abnormal size | adult stage phenotype, suppressible by Bger\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^phtm.PO, Sumo^RNAi.UAS has lethal - all die during larval stage phenotype, suppressible by Bger\SUMO3^UAS.cUa, Scer\GAL4^phtm.PO

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has visible | adult stage phenotype, suppressible by Bger\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has visible | adult stage phenotype, non-suppressible by Bger\SUMO1^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has abnormal size | adult stage phenotype, non-suppressible by Bger\SUMO1^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^phtm.PO, Sumo^RNAi.UAS has lethal - all die during larval stage phenotype, non-suppressible by Bger\SUMO1^UAS.cUa, Scer\GAL4^phtm.PO

(Ureña et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has wing phenotype, enhanceable by Hsap\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has submarginal cell phenotype, enhanceable by Hsap\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has wing phenotype, suppressible by Bger\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has submarginal cell phenotype, suppressible by Bger\SUMO3^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^phtm.PO, Sumo^RNAi.UAS has prothoracic gland phenotype, suppressible by Scer\GAL4^phtm.PO/Snmp1^UAS.cBa

(Talamillo et al., 2013)

Scer\GAL4^phtm.PO, Sumo^RNAi.UAS has lipid droplet phenotype, suppressible by Scer\GAL4^phtm.PO/Snmp1^UAS.cBa

(Talamillo et al., 2013)

Scer\GAL4^T155, Sumo^RNAi.UAS has follicle cell phenotype, suppressible | partially by Scer\GAL4^T155/Snmp1^UAS.cBa

(Talamillo et al., 2013)

Scer\GAL4^T155, Sumo^RNAi.UAS has lipid droplet phenotype, suppressible | partially by Scer\GAL4^T155/Snmp1^UAS.cBa

(Talamillo et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has wing phenotype, non-suppressible by Bger\SUMO1^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Scer\GAL4^salm.EPv, Sumo^RNAi.UAS has submarginal cell phenotype, non-suppressible by Bger\SUMO1^UAS.cUa, Scer\GAL4^salm.EPv

(Ureña et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Snmp1^Scer\UAS.cBa partially rescues the reduction in the number of lipid droplets per cell in the prothoracic glands of 51% of larvae expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO. The lipid droplets are comparable in size to controls. Expression of Snmp1^Scer\UAS.cBa also rescues the lipid content in follicle cells expressing smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^T155.

(Talamillo et al., 2013)

Xenogenetic Interactions

Statement

Reference

The decreases in area of the full wing and, in particular, of the intervein region between L2 and L3 resulting from the expression of smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^salm.EPv, are enhanced, fully suppressed, or unaffected by co-expression of Hsap\SUMO3^Scer\UAS.cUa, Bger\SUMO3^Scer\UAS.cUa, or Bger\SUMO1^Scer\UAS.cUa, respectively. The development arrest at the larval stage resulting from the expression of smt3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^phm.PO is fully suppressed by the co-expression of Bger\SUMO1^Scer\UAS.cUa, but not Bger\SUMO3^Scer\UAS.cUa.

(Ureña et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sumo^RNAi.UAS

Sumo^dsRNA.UAS

smt3^{dsRNA.Scer\UAS}

smt3^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

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