A limited number of adult flies can be obtained when Cul-3^NIG.11861R is expressed under the control of Scer\GAL4^Clk.8424.

Under light-dark conditions, flies expressing Cul-3^NIG.11861R under the control of either Scer\GAL4^P2.4.Pdf or Scer\GAL4^Clk.8424 show no or reduced morning anticipatory activity. After transfer to constant darkness conditions, the flies either show weak locomotor activity rhythms or become arrhythmic.

Flies expressing Cul-3^NIG.11861R under the control of Scer\GAL4^P2.4.Pdf which are raised at 25[o]C and tested at 20[o]C show weaker locomotor activity rhythm defects under constant darkness conditions than flies raised and tested at 25[o]C, indicating that Cul-3 function is required during the adult stage for normal locomotor activity rhythms.

Flies expressing Cul-3^NIG.11861R under the control of Scer\GAL4¹¹¹⁸ show reduced morning anticipation under light-dark conditions. Under constant darkness conditions, only 44% of the flies are rhythmic for locomotor activity and the rhythmic flies display weak rhythms.

Under constant darkness conditions, only 30% of flies expressing Cul-3^NIG.11861R under the control of Scer\GAL4^cry.PE are rhythmic for locomotor activity and the rhythmic flies display weak rhythms.

Expression of Cul-3^NIG.11861R under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi effects) results in sleep defects: the flies show a reduction in sleep duration compared to controls, with a reduction in the average sleep bout length and an increase in latency to sleep after lights-off. The mutant flies show defects in sleep homeostasis, lacking a detectable sleep rebound after sleep deprivation. The mutant flies are more easily woken by a rotational stimulus compared to wild type controls.

Expression of Cul-3^NIG.11861R under the control of Scer\GAL4^30Y (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi effects) has no affect on sleep.

Expression of Cul-3^NIG.11861R under the control of Scer\GAL4^{elav.Switch.PO} during development (using RU486 to control the timing of expression and in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi effects) results in reduced sleep duration, while expression of inc^KK108908 under the control of Scer\GAL4^{elav.Switch.PO} during the adult stage only does not result in sleep defects.

Expression of Cul-3^NIG.11861R under the control of Scer\GAL4^elav-C155 results in a small decrease in sleep duration. In the presence of Dcr-2^Scer\UAS.cDa (to enhance the strength of RNAi) these animals show a severe decrease in sleep duration and bout length compared to controls.