Amino acid replacement: Q80term.
C15226181T
Q80term | Caf1-55-PA; Q79term | Caf1-55-PB
Q80term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
lethal (with Caf1-55long)
lethal (with Caf1-55med)
lethal (with Df(3R)ED5664)
visible | cold sensitive (with Caf1-55med), with Caf1-55UAS.cAa, Scer\GAL4Ubi.PU
arista | cold sensitive (with Caf1-55med), with Caf1-55UAS.cAa, Scer\GAL4Ubi.PU
eye | cold sensitive (with Caf1-55med), with Caf1-55UAS.cAa, Scer\GAL4Ubi.PU
leg | ectopic | cold sensitive (with Caf1-55med), with Caf1-55UAS.cAa, Scer\GAL4Ubi.PU
rhabdomere | cold sensitive (with Caf1-55med), with Caf1-55UAS.cAa, Scer\GAL4Ubi.PU
Homozygous larvae appear grossly normal but mature slowly and die as first or second instars.
Homozygous clones induced using hs-FLP do not survive in adults. Homozygous clones in the eye which have been generated using the ey-FLP; FRT cell lethal system are very small, comprising only a small portion of the eye. The remaining eye is small, highly disorganised and almost completely devoid of bristles. Approximately 20% of flies containing ey-FLP induced clones have extra or missing appendages on the head, including the ocelli, antennae and maxillary palps.
Homozygous clones in the third larval instar eye disc which have been generated using the ey-FLP; FRT cell lethal system are small, show extensive apoptosis and have an irregular arrangement of photoreceptor clusters.
Homozygous clones have not been recovered in the adult thorax.
Caf1short/Caf1med flies expressing Caf1Scer\UAS.cAa under the control of Scer\GAL4Ubi and raised at 18[o]C during the third larval instar stage have disorganised kidney-shaped eyes with abnormalities in the arrangement of rhabdomeres (including loss of small central rhabdomeres) and show homeotic transformation of the arista to leg-like structures that occasionally end in claws. These defects are not seen if the animals are raised at 25[o]C during the third larval instar stage.
Caf1-55short is an enhancer of increased cell number phenotype of numbEx112/numb15
Caf1-55short/Caf1[+] is a suppressor of visible phenotype of Scer\GAL4lz-gal4, sensUAS.cNa
Caf1-55short/Caf1[+] is a suppressor of visible | dominant phenotype of Pc15
Caf1-55short is an enhancer of type II neuroblast | increased number phenotype of numbEx112/numb15
Caf1-55short/Caf1[+] is a suppressor of eye phenotype of Scer\GAL4lz-gal4, sensUAS.cNa
Caf1-55short/Caf1[+] is a suppressor of sex comb | ectopic phenotype of Pc15
Caf1-55short/Caf1[+] is a suppressor of mesothoracic leg phenotype of Pc15
Caf1-55short/Caf1[+] is a suppressor of metathoracic leg phenotype of Pc15
The mutant eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4 is dominantly suppressed by Caf1short.
Only 12.3% of Pc15/Caf1short double heterozygous males have ectopic sex combs on either the second or third leg.
Caf1-55short/Caf1-55long is rescued by Scer\GAL4Ubi.PU/Caf1-55UAS.cAa
Caf1-55med/Caf1-55short is rescued by Caf1-55+t12
Caf1-55short/Df(3R)ED5664 is rescued by Caf1-55+t12
Caf1-55med/Caf1-55short is partially rescued by Scer\GAL4Ubi.PU/Caf1-55UAS.cAa
Caf1-55short is partially rescued by Scer\GAL4Ubi.PU/Caf1-55UAS.cAa