FB2024_03 , released June 25, 2024
Allele: Dmel\Spc105RGL00392
Open Close
General Information
Symbol
Dmel\Spc105RGL00392
Species
D. melanogaster
Name
FlyBase ID
FBal0262858
Feature type
allele
Associated gene
Dmel\Spc105R
Associated Insertion(s)
Carried in Construct
P{TRiP.GL00392}
Also Known As
GL00392, Spc105R RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
in vitro construct
(Ni et al., 2011.7.19)
Progenitor genotype
Carried in construct
P{TRiP.GL00392}
Cytology
Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-GL00392
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  amyotrophic lateral sclerosis type 8
      modeled by Vap33P58S.UAS.Tag:FLAG
      (Sanhueza et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Spc105RGL00392 under the control of Scer\GAL4VP16.mat.αTub67C does not significantly affect karyosome compaction in late-stage oocytes, as compared to controls.

      (Radford et al., 2017)

      Expression of Spc105RGL00392 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 in oocytes disrupts kinetochore assembly, kinetochore-microtubule attachment and interactions, a loss of co-orientation of homologous chromosomes, lack of elongation of the karyosome, lack of separation of achiasmate chromosomes, lack of centromere orientation towards the spindle pole, weak/absent prometaphase and metaphase spindles, in contrast to wild type, no defect in central spindle formation or bipolar spindle morphology at prometaphase, but exhibit disorganization of the central spindle at metaphase.

      Expression of Spc105RGL00392 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in females results in sterility.

      (Radford et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of subGL00583 enhances the weak/absent spindle phenotype in prometaphase oocytes expressing Spc105RGL00392 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      Co-expression of Spc105RGL00392 suppresses the karyosome splitting defect of oocytes expressing cmetGL00404 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16.

      (Radford et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Spc105RGL00392
      Name Synonyms
      Secondary FlyBase IDs
        References (7)