FlyBase Allele Report: Dmel\cmet[GL00404]

General Information

Symbol

Dmel\cmet^GL00404

Species

D. melanogaster

Name

FlyBase ID

FBal0262870

Feature type

allele

Associated gene

Dmel\cmet

Associated Insertion(s)

Carried in Construct

P{TRiP.GL00404}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.GL00404}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-GL00404

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2015)

karyosome, with Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2015)

Detailed Description

Statement

Reference

Expression of cmet^GL00404 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} leads to a subset of oocytes exhibiting karyosome splitting, with frequent splits into multiple masses, and mis-orientation of homologous chromosomes, but no significant defects in end-on kinetochore-microtubule attachment formation.

(Radford et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, cmet^GL00404 has karyosome phenotype, enhanceable by Ndc80^GL00625, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, cmet^GL00404 has karyosome phenotype, suppressible by Spc105R^GL00392/Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Spc105R^GL00392 suppresses the karyosome splitting defect of oocytes expressing cmet^GL00404 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

Co-expression of Ndc80^GL00625 enhances the karyosome splitting defect of oocytes expressing cmet^GL00404 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in karyosome splitting.

(Radford et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

35816