FlyBase Allele Report: Dmel\lola[22.05]

General Information

Symbol

Dmel\lola^22.05

Species

D. melanogaster

Name

FlyBase ID

FBal0260764

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Tripathy et al., 2014)

Mutagen

ethyl methanesulfonate

(Weyers et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q97term.

(Silva et al., 2016)

Predicted to affect all lola isoforms.

(Silva et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:10,533,397..10,533,397 [-]

Nucleotide change:

C10533397T

Amino acid change:

Reported amino acid change:

Q97term

Comment:

Affects all lola isoforms. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Silva et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage (with lola^46.38)

(Silva et al., 2016)

lethal (with lola^C28)

(Tripathy et al., 2014)

Phenotype Manifest In

germline cell | embryonic stage (with lola^46.38)

(Silva et al., 2016)

germline cell | embryonic stage (with lola^e76)

(Silva et al., 2016)

germline cell | embryonic stage (with lola^ORC4)

(Silva et al., 2016)

gonad | embryonic stage

(Tripathy et al., 2014)

gonad | embryonic stage (with lola^46.38)

(Silva et al., 2016, Weyers et al., 2011)

gonad | embryonic stage (with lola^C28)

(Tripathy et al., 2014)

gonad | embryonic stage (with lola^e76)

(Silva et al., 2016)

gonad | embryonic stage (with lola^ORC4)

(Silva et al., 2016)

gonadal sheath proper primordium (with lola^46.38)

(Silva et al., 2016)

gonadal sheath proper primordium (with lola^e76)

(Silva et al., 2016)

gonadal sheath proper primordium (with lola^ORC4)

(Silva et al., 2016)

gonadal sheath proper primordium | embryonic stage (with lola^46.38)

(Weyers et al., 2011)

larval longitudinal connective

(Tripathy et al., 2014)

Detailed Description

Statement

Reference

lola^22.05/lola^46.38, lola^22.05/lola^e76 and lola^22.05/lola^ORC4 transheterozygous embryos, but not lola^22.05 heterozygotes, display a significant increase in defects in the fusion together and compaction of primordial germ cells and somatic gonadal precursor cells, as compared to controls.

lola^22.05/lola^46.38 transheterozygous embryos also display a significant increase in the number of extragonadal primordial germ cells, but no obvious defects in the development of the visceral mesoderm, as compared to controls.

(Silva et al., 2016)

Homozygous lola^22.05 mutant embryos exhibit defects in gonad development.

lola^C28/lola^22.05 mutant embryos exhibit gonad development defects that are similar in penetrance and severity to either lola^C28 or lola^22.05 homozygotes.

Homozygous lola^22.05 mutant embryos display interruptions in the longitudinal axonal tracts of the ventral nerve cord.

(Tripathy et al., 2014)

lola^22.05/lola^46.38 embryos have gonads with at least one somatic gonadal precursor (SGP) cluster that does not fuse with other SGPs. Somatic gonadal precursor specification appears normal. Visceral mesoderm appears normal.

(Weyers et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

lola^46.38/lola^22.05 has gonad | embryonic stage phenotype, enhanceable by rib^55.25/rib[+]

(Silva et al., 2016)

lola^46.38/lola^22.05 has gonadal sheath proper primordium phenotype, enhanceable by rib^55.25/rib[+]

(Silva et al., 2016)

lola^46.38/lola^22.05 has germline cell | embryonic stage phenotype, enhanceable by rib^55.25/rib[+]

(Silva et al., 2016)

Enhancer of

Statement

Reference

lola[+]/lola^22.05 is an enhancer of gonad | embryonic stage phenotype of rib^35.14/rib^55.25

(Silva et al., 2016)

lola[+]/lola^22.05 is an enhancer of gonadal sheath proper primordium phenotype of rib^35.14/rib^55.25

(Silva et al., 2016)

lola[+]/lola^22.05 is an enhancer of germline cell | embryonic stage phenotype of rib^35.14/rib^55.25

(Silva et al., 2016)

Other

Statement

Reference

lola^22.05, rib^35.14/rib[+] has gonadal sheath proper primordium phenotype

(Silva et al., 2016)

lola^22.05, rib^35.14/rib[+] has gonad | embryonic stage phenotype

(Silva et al., 2016)

lola^22.05, rib^35.14/rib[+] has germline cell | embryonic stage phenotype

(Silva et al., 2016)

Scer\GAL4^twi.PG, lola^C28/lola^22.05, mid^UAS.cBa has fat body/gonad primordium phenotype

(Tripathy et al., 2014)

Scer\GAL4^twi.PG, lola^C28/lola^22.05, mid^UAS.cBa has gonad | embryonic stage phenotype

(Tripathy et al., 2014)

Scer\GAL4^twi.PG, lola^C28/lola^22.05, mid^UAS.cBa has gonadal sheath proper primordium | increased number phenotype

(Tripathy et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

lola^22.05/+, rib^35.14/+ double heterozygous embryos display a very significant increase in defects in the fusion together and compaction of primordial germ cells and somatic gonadal precursor cells, as compared to controls.

Heterozygosity for lola^22.05 significantly enhances the defects exhibited by rib^35.14/rib^55.25 transheterozygous embryos to fuse together and compact primordial germ cells and somatic gonadal precursor cells.

(Silva et al., 2016)

When mid^Scer\UAS.cBa is expressed in the mesoderm of lola^C28/lola^22.05 mutant embryos under the control of Scer\GAL4^twi.PG 'super-elongated' gonads are produced in more than 50% of stage 15 embryos. Although somatic gonadal precursor (SGP) cluster formation occurs, these gonads remain elongated, spanning several parasegments. Far more SGPs are seen than in sibling controls, and these extra SGPs arise from conversion from fat body cells at stage 13.

(Tripathy et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lola^C28/lola^22.05 is rescued by lola^UAS.R.GFP/Scer\GAL4^twi.PG

(Tripathy et al., 2014)

Not rescued by

lola^C28/lola^22.05 is not rescued by Scer\GAL4^twi.PG/lola^L.UAS

(Tripathy et al., 2014)

Comments

Expression of lola^{Scer\UAS.R.T:Avic\GFP} in the mesoderm under the control of Scer\GAL4^twi.PG rescues the gonad defects of lola^C28/lola^22.05 mutant embryos, leading to round and compact stage 15 gonads and few germ cells that have not been ensheathed by somatic gonadal precursor cells.

Expression of lola^L.Scer\UAS in the mesoderm under the control of Scer\GAL4^twi.PG does not rescue the gonad defects of lola^C28/lola^22.05 mutant embryos.

Expression of lola^{Scer\UAS.R.T:Avic\GFP} in the mesoderm under the control of Scer\GAL4^twi.PG rescues the gonad defects of homozygous lola^22.05 mutant embryos.

(Tripathy et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

lola^22.05

(Silva et al., 2016, Tripathy et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (3)

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