FB2024_03 , released June 25, 2024
Allele: Dmel\tkvca.UASp
Open Close
General Information
Symbol
Dmel\tkvca.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0242615
Feature type
allele
Associated gene
Dmel\tkv
Associated Insertion(s)
Carried in Construct
P{UASp-tkv.ca}
Also Known As
P{uasp-tkv(ca)}, UASp-tkvCA
Key Links
Transgenic product class
missense_variant
(Jiang et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Jiang et al., 2008)
Mutagen
in vitro construct
(Jiang et al., 2008)
Progenitor genotype
Carried in construct
P{UASp-tkv.ca}
Cytology
Description

Amino acid replacement: Q253D.

(Jiang et al., 2008)

UASp regulatory sequences drive expression of a constitutively active form of tkv.

(Jiang et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
tkv
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Although mean cell number increases in MARCM clones expressing Scer\GAL4tub.PU>tkvca.Scer\UAS.P\T 24 hours after bleomycin treatment, the tkvca.Scer\UAS.P\T-expressing clones have fewer cells than wild-type clones after 48 and 72 hours.

      (Guo et al., 2013)

      Expression of tkvca.Scer\UAS.P\T in germline cells using Scer\GAL4nos.UTR.T:Hsim\VP16 results in the germaria containing an average of 2.6 cystoblasts per germanium, in comparison with about one cystoblast per wild-type germarium.

      (Chen et al., 2010)

      The germaria of flies expressing tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 contain an increased number of spectrosome-containing GSC-like cells.

      (Xia et al., 2010)

      Expression of tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in females results in a tumorous germarium consisting of undifferentiated germline stem cell-like cells that carry spectrosomes.

      (Jiang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      Statement
      Reference

      Scer\GAL4VP16.nanos.UTR/tkvca.UASp is a suppressor of female germline stem cell phenotype of dallygem

      (Guo and Wang, 2009)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Lis-1D heterozygous germaria overexpressing tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exhibit significantly fewer cystoblasts than wild-type germaria overexpressing tkvca.Scer\UAS.P\T.

      (Chen et al., 2010)

      Expression of fuScer\UAS.P\T.T:Myr-Src64B partially suppresses the increase in spectrosome-containing GSC-like cells seen when tkvca.Scer\UAS.P\T is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16. The germaria contain branched fusomes and 30% of ovarioles carry normal egg chambers.

      Co-expression of lackScer\UAS.P\T.cXa and fuScer\UAS.P\T.T:Myr-Src64B strongly suppresses the increase in spectrosome-containing GSC-like cells seen when tkvca.Scer\UAS.P\T is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16. The germaria contain branched fusomes and nearly 50% of egg chambers contain normal egg chambers.

      (Xia et al., 2010)

      Expression of tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 suppresses the loss of germline stem cells which is seen in the ovarioles of dallygem females.

      (Guo and Wang, 2009)

      Introduction of one copy of Oteunspecified into females expressing tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in some of the germline stem cell-like cells in the ovaries differentiating into cyst cells with highly branched fusomes. The germaria are attached with a long string of egg chambers and fertility is restored.

      Females expressing tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in a homozygous Oteunspecified background have a phenotype that is similar to that found in Oteunspecified single mutants; the majority of germaria are either empty or contain only differentiated germ cells.

      (Jiang et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      tkvca.Scer\UAS.P\T
      tkvca.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (7)