FlyBase Allele Report: Dmel\Ptp69D[JF03399]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\Ptp69D^JF03399

General Information

Symbol

Dmel\Ptp69D^JF03399

Species

D. melanogaster

Name

FlyBase ID

FBal0242570

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03399}

Also Known As

Ptp69D RNAi

Key Links

Genomic Maps

Transgenic product class

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03399}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Pun et al., 2022)

exacerbates muscular dystrophy-dystroglycanopathy

modeled by tw^GD1160

(Monagas-Valentin et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

decreased size | adult stage, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

Phenotype Manifest In

adult thoracic mechanosensory chaeta neuron, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

axon collateral | adult stage, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

axon terminus | adult stage | decreased number, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

presynapse | adult stage | decreased number, with Scer\GAL4^pnr-MD237

(Landínez-Macías et al., 2021)

Detailed Description

Statement

Reference

The expression of Ptp69D^JF03399 under the control of Scer\GAL4^pnr-MD237 results in adult thoracic mechanosensory neurons with decreased connectivity: full extension of the contralateral branch, but complete loss of synaptic arborizations; decreased presynapse number; and contralateral branch arrest at the midline/on the ipsilateral side.

(Landínez-Macías et al., 2021)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ptp69D^JF03399/Scer\GAL4^{Lar-MI03443-TG4.1} is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Lar^CRISPR.3/Lar^{MI03443-TG4.1}

(Poe et al., 2017)

NOT Enhancer of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^repo.PU is a non-enhancer of increased size | larval stage phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Suppressor of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^elav.PU is a suppressor of increased size | larval stage phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

NOT Suppressor of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^repo.PU is a non-suppressor of increased size | larval stage phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Other

Statement

Reference

Lar^{MI03443-TG4.1}, Ptp69D^JF03399, Scer\GAL4^{Lar-MI03443-TG4.1} has abnormal neuroanatomy | third instar larval stage phenotype

(Poe et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Ptp69D^JF03399/Scer\GAL4^{Lar-MI03443-TG4.1} is an enhancer of larval multidendritic class IV neuron | third instar larval stage phenotype of Lar^CRISPR.3/Lar^{MI03443-TG4.1}

(Poe et al., 2017)

Ptp69D^JF03399/Scer\GAL4^{Lar-MI03443-TG4.1} is an enhancer of dendritic tree | third instar larval stage phenotype of Lar^CRISPR.3/Lar^{MI03443-TG4.1}

(Poe et al., 2017)

NOT Enhancer of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^repo.PU is a non-enhancer of larval ventral nerve cord phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Suppressor of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^elav.PU is a suppressor of larval ventral nerve cord phenotype of Scer\GAL4^elav.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

NOT Suppressor of

Statement

Reference

Ptp69D^JF03399, Scer\GAL4^repo.PU is a non-suppressor of larval ventral nerve cord phenotype of Scer\GAL4^repo.PU, kon^{UAS.Tag:HA,Tag:SS(wg)}

(Harrison et al., 2021)

Other

Statement

Reference

Lar^CRISPR.3, Ptp69D^JF03399, Scer\GAL4^{Lar-MI03443-TG4.1} has distal dendrite | third instar larval stage phenotype

(Poe et al., 2017)

Lar^{MI03443-TG4.1}, Ptp69D^JF03399, Scer\GAL4^{Lar-MI03443-TG4.1} has larval multidendritic class IV neuron | third instar larval stage phenotype

(Poe et al., 2017)

Lar^{MI03443-TG4.1}, Ptp69D^JF03399, Scer\GAL4^{Lar-MI03443-TG4.1} has dendritic tree | third instar larval stage phenotype

(Poe et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The very slight decrease in total dendrite density in class IV dendritic arborizing (da) neurons characteristic for third instar larvae transheterozygous for Lar^CRISPR.3/Lar^{MI03443-TG4.1} is strongly enhanced by expression of Ptp69D^JF03399 under the Scer\GAL4^{Lar-MI03443-TG4.1} driver (which is inherent to the Lar^{MI03443-TG4.1} allele), in addition the terminal dendrite density and terminal dendrite numbers are also significantly reduced. Larvae heterozygous for Lar^{MI03443-TG4.1} and expressing Ptp69D^JF03399 (under the Scer\GAL4^{Lar-MI03443-TG4.1}) driver display strong reduction in the total and terminal dendrite density as well as terminal dendrite numbers in the class IV da neurons relative to controls, this phenotype is not further worsened by combination with Lar^CRISPR.3.

(Poe et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

29462

y¹ v¹; P{TRiP.JF03399}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PTP69D^RNAi

(Monagas-Valentin et al., 2023)

Ptp69D^JF03399

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections