FB2024_03 , released June 25, 2024
Allele: Dmel\LarCRISPR.3
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General Information
Symbol
Dmel\LarCRISPR.3
Species
D. melanogaster
Name
FlyBase ID
FBal0336321
Feature type
allele
Associated gene
Dmel\Lar
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Poe et al., 2017)
Progenitor genotype
Cytology
Description

Large deletion that removes parts of the extracellular domain, the transmembrane domain, the first intracellular phosphatase domain, and parts of the second phosphatase domain.

(Poe et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:19,727,122..19,729,629 [+]
Comment:

2508 bp deletion in Lar that removes parts of the extracellular domain, the transmembrane domain, the first intracellular phosphatase domain, and parts of the second phosphatase domain.

(Poe et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      LarCRISPR.3/LarMI03443-TG4.1 transheterozygous third instar larvae show very mild decrease in total dendrite density (but not in terminal dendrite density or numbers), while LarCRISPR.13/LarCRISPR.3 transheterozygotes display slight increase in both total dendrite density and terminal dendrite numbers relative to controls.

      (Poe et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The very slight decrease in total dendrite density in class IV dendritic arborizing neurons characteristic for third instar larvae transheterozygous for LarCRISPR.3/LarMI03443-TG4.1 is strongly enhanced by expression of Ptp69DJF03399 under the Scer\GAL4Lar-MI03443-TG4.1 driver (which is inherent to the LarMI03443-TG4.1 allele), in addition the terminal dendrite density and terminal dendrite numbers are also significantly reduced.

      (Poe et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Lar3
      (Poe et al., 2017)
      LarCRISPR.3
      Name Synonyms
      Secondary FlyBase IDs
        References (1)