FlyBase Allele Report: Zzzz\CAG[Q92.GMR.Tag:FLAG]

General Information

Symbol

Zzzz\CAG^{Q92.GMR.Tag:FLAG}

Species

a. artificial

Name

FlyBase ID

FBal0240457

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{GMR-Q92}

Also Known As

gmr-Q92, GMR-FlagQ92

Transgenic product class

trinucleotide_repeat_expansion

(Higashiyama et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Higashiyama et al., 2002)

Mutagen

in vitro construct

(Higashiyama et al., 2002)

Progenitor genotype

Carried in construct

P{GMR-Q92}

Cytology

Description

GMR regulatory sequences drive expression of a 92 residue polyglutamine repeat, which is tagged with Tag:FLAG.

(Higashiyama et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Zzzz\CAG

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Manno et al., 2010, Nagai et al., 2003, Higashiyama et al., 2002)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is exacerbated by Hsap\VCP^A232E.UAS

(Manno et al., 2010)

is exacerbated by Hsap\VCP^R155P.UAS

(Manno et al., 2010)

is exacerbated by Hsap\VCP^T761E.UAS

(Manno et al., 2010)

is exacerbated by Hsap\VCP^R155H.UAS

(Manno et al., 2010)

is ameliorated by Hsap\VCP^UAS.cMa

(Manno et al., 2010)

is ameliorated by TER94^26-8

(Higashiyama et al., 2002)

is exacerbated by TER94^UAS.cHa

(Higashiyama et al., 2002)

is ameliorated by Hsap\PQBP1^UAS.CFP

(Nagai et al., 2003)

is ameliorated by Mmmm\VCP^UAS.cKa

(Koike et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Tue et al., 2012, Koike et al., 2010, Manno et al., 2010, Higashiyama et al., 2002)

Phenotype Manifest In

eye

(Tue et al., 2012, Koike et al., 2010, Manno et al., 2010, Higashiyama et al., 2002)

Detailed Description

Statement

Reference

Flies carrying Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} show eye degeneration phenotypes, including loss of pigment and rough eyes. Inclusion bodies are seen in the region posterior to the morphogenetic furrow in the eye disc.

(Tue et al., 2012)

Flies carrying Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} show degeneration of the eye.

(Koike et al., 2010)

Expression of Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} induces eye degeneration.

(Manno et al., 2010)

Flies carrying Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} have a rough eye phenotype, and reduced pigmentation in the eye. The internal structure of the compound eye is severely disrupted, with most eye cells being shrunken or flattened.

(Higashiyama et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^T761E.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^R155H.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^R155P.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^A232E.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, enhanceable by Scer\GAL4^GMR.PS

(Higashiyama et al., 2002)

Suppressed by

Statement

Reference

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible by Scer\GAL4^GMR.PS/Hsap\CRYAB^UAS.Tag:HA

(Tue et al., 2012)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible by Scer\GAL4^GMR.PS/Mmmm\VCP^UAS.cKa

(Koike et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible by Scer\GAL4^GMR.PS/Hsap\VCP^UAS.cMa

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible | partially by TER94⁰³⁷⁷⁵/TER94[+]

(Higashiyama et al., 2002)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible | partially by TER94[+]/TER94^26-8

(Higashiyama et al., 2002)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has visible phenotype, suppressible | partially by TER94[+]/TER94^22-26

(Higashiyama et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^T761E.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^R155H.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^R155P.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/Hsap\VCP^A232E.UAS

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, enhanceable by Scer\GAL4^GMR.PS

(Higashiyama et al., 2002)

Suppressed by

Statement

Reference

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible by Scer\GAL4^GMR.PS/Hsap\CRYAB^UAS.Tag:HA

(Tue et al., 2012)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible by Scer\GAL4^GMR.PS/Mmmm\VCP^UAS.cKa

(Koike et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible by Scer\GAL4^GMR.PS/Hsap\VCP^UAS.cMa

(Manno et al., 2010)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible | partially by TER94⁰³⁷⁷⁵/TER94[+]

(Higashiyama et al., 2002)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible | partially by TER94[+]/TER94^26-8

(Higashiyama et al., 2002)

Zzzz\CAG^{Q92.GMR.Tag:FLAG} has eye phenotype, suppressible | partially by TER94[+]/TER94^22-26

(Higashiyama et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\CRYAB^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PS effectively suppresses the mutant eye phenotypes of Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} flies, especially the loss of pigment phenotype. The formation of inclusion bodies in the eye disc is also suppressed in these animals.

(Tue et al., 2012)

Expression of Mmmm\VCP^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS suppresses the eye degeneration caused by Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}.

(Koike et al., 2010)

Co-expression of Scer\GAL4^GMR.PS>Hsap\VCP^{T761E.Scer\UAS} with Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} enhances the Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}-induced eye degeneration.

Co-expression of Scer\GAL4^GMR.PS>Hsap\VCP^{R155H.Scer\UAS} with Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} enhances the Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}-induced eye degeneration.

Co-expression of Scer\GAL4^GMR.PS>Hsap\VCP^{R155P.Scer\UAS} with Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} enhances the Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}-induced eye degeneration.

Co-expression of Scer\GAL4^GMR.PS>Hsap\VCP^{A232E.Scer\UAS} with Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} enhances the Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}-induced eye degeneration.

Co-expression of Scer\GAL4^GMR.PS>Hsap\VCP^Scer\UAS.cMa with Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG} mitigates the Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}-induced eye degeneration.

(Manno et al., 2010)

Expression of TER94^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PS enhances the rough eye phenotypes caused by Zzzz\CAG^{Q92.GMR.T:Zzzz\FLAG}.

(Higashiyama et al., 2002)

Complementation and Rescue Data