FlyBase Allele Report: Dmel\Tip60[HM05049]

General Information

Symbol

Dmel\Tip60^HM05049

Species

D. melanogaster

Name

FlyBase ID

FBal0239381

Feature type

allele

Associated gene

Dmel\Tip60

Associated Insertion(s)

Carried in Construct

P{TRiP.HM05049}

Also Known As

Tip60 RNAi, UAS-Tip60 RNAi, HM05049

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.HM05049}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HM05049

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates hematologic cancer

modeled by hop^Tum

(Bailetti et al., 2019)

exacerbates acute myeloid leukemia

modeled by Hsap\HOXA9::Hsap\NUP98^WT.UAS

(Gavory et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Tip60^HM05049 under the control of Scer\GAL4^NP1624 leads to testes showing increased numbers of cyst stem cells (Zfh-1-positive cells), associated with cyst stem cell differentiation defects, as illustrated by a high proportion of cells abnormally co-expressing Zfh-1 (marker of cyst stem cells/early cyst cells) and Eya (marker of later stage cyst cells). About half of testes also show germ cell expansion, with some testes also showing structures that resemble stem cell, GB and spermatogonial tumors. Additionally, some testes show cells expressing both germ cell markers (Vasa) and cyst cell markers (Zfh-1 or Yan).

(Feng et al., 2017)

Expression of Tip60^HM05049 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{otu.T:Hsim\VP16} causes significantly decreased female fertility, with germline development in the germarium appearing normal, but later stage egg chambers appearing defective, as compared to controls.

Expression of Tip60^HM05049 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not affect the development of the germarium or ovariole, but results in a significant proportion of eggs showing the 'dumpless' egg phenotype, as shown by defective late stage egg chambers with short eggs and non-fragmented nurse cells, and a significant reduction in egg-laying, as compared to controls.

(Börner and Becker, 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Switch1.106, Tip60^HM05049 has short lived phenotype, suppressible by foxo^UAS.cFa/Scer\GAL4^Switch1.106

(Martínez Corrales et al., 2022)

Enhancer of

Statement

Reference

Tip60^HM05049, Scer\GAL4^NP1624 is an enhancer of neoplasia | spermatogenesis phenotype of E(Pc)^GL00169, Scer\GAL4^NP1624

(Feng et al., 2017)

Scer\GAL4^bbg-C96/Tip60^HM05049 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Suppressor of

Statement

Reference

Tip60^HM05049/Scer\GAL4^Switch1.106 is a suppressor of long lived | female phenotype of Scer\GAL4^Switch1.106, foxo^UAS.cFa

(Martínez Corrales et al., 2022)

Other

Statement

Reference

Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH, Tip60^HM05049 has visible | adult stage phenotype

(Gavory et al., 2021)

Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH, Tip60^HM05049 has abnormal size | adult stage phenotype

(Gavory et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Tip60^HM05049, Scer\GAL4^NP1624 is an enhancer of male germline cell | increased number phenotype of E(Pc)^GL00169, Scer\GAL4^NP1624

(Feng et al., 2017)

Scer\GAL4^bbg-C96/Tip60^HM05049 is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Other

Statement

Reference

Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH, Tip60^HM05049 has wing | ectopic phenotype

(Gavory et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Hsap\HOXA9::Hsap\NUP98^WT.UAS and Tip60^HM05049 under the control of Scer\GAL4^ey.PH leads to ectopic wing-like structures in the dorsal-anterior part of the eye.

(Gavory et al., 2021)

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also co-expressing Tip60^HM05049.

(Kwon et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28563

y¹ v¹; P{TRiP.HM05049}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tip60^HM05049

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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