FlyBase Allele Report: Dmel\Rala[S25N.cMa.UAS]

General Information

Symbol

Dmel\Rala^S25N.cMa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0221633

Feature type

allele

Associated gene

Dmel\Rala

Associated Insertion(s)

Carried in Construct

P{UAS-Ral.S25N.M}

Key Links

Transgenic product class

dominant_negative_variant

(Mirey et al., 2003)

missense_variant

(Mirey et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Mirey et al., 2003)

missense_variant

(Mirey et al., 2003)

Mutagen

in vitro construct

(Mirey et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Ral.S25N.M}

Cytology

Description

UAS regulatory sequences drive expression of a dominant negative form of Rala.

(Mirey et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rala

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^da.G32

(Mirey et al., 2003)

visible, with Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

visible, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

Phenotype Manifest In

adult head bristle, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

macrochaeta, with Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

macrochaeta, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

microchaeta, with Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

microchaeta, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

trichogen cell, with Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Detailed Description

Statement

Reference

Adults expressing Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca-537.4 show a loss of shaft cells in the microchaetae and macrochaetae.

(Balakireva et al., 2006)

Expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in a mutant phenotype in the eye.

Expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU results in the loss of the bristle shafts of most microchaetae and macrochaetae on the notum and the head.

(Mirey et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, enhanceable by Rlip^UAS.WT, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, enhanceable by msn^{Δ332-667.UAS}, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has visible phenotype, enhanceable by Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has visible phenotype, enhanceable by Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT Enhanced by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, suppressible by Jra^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, suppressible by p38b^UAS.cAa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, suppressible | partially by bsk¹/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, suppressible | partially by bsk²/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has visible phenotype, suppressible by Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has visible phenotype, non-suppressible by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Enhancer of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

NOT Enhancer of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Suppressor of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU is a suppressor | partially of lethal | embryonic stage phenotype of Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

NOT Suppressor of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU is a non-suppressor of lethal phenotype of Ras85D^V12.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Other

Statement

Reference

Rala^S25N.cMa.UAS, Ras85D^V12.S35.UAS, Scer\GAL4^sca.PU has lethal | pupal stage phenotype

(Mirey et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, enhanceable by p38b^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, enhanceable by Rlip^UAS.WT, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, enhanceable by Rlip^UAS.WT, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, enhanceable by msn^{Δ332-667.UAS}, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, enhanceable by msn^{Δ332-667.UAS}, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has microchaeta phenotype, enhanceable by Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has macrochaeta phenotype, enhanceable by Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has microchaeta phenotype, enhanceable by Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has macrochaeta phenotype, enhanceable by Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT Enhanced by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-enhanceable by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-enhanceable by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-enhanceable by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-enhanceable by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, suppressible | partially by bsk¹/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, suppressible by bsk¹/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, suppressible | partially by bsk²/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, suppressible by bsk²/bsk[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, suppressible by Jra^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, suppressible by Jra^DN.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, suppressible by p38b^UAS.cAa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, suppressible by p38b^UAS.cAa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has microchaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has macrochaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Ras85D^V12.C40.UAS, Scer\GAL4^sca.PU has chaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Ras85D^V12.G37.UAS, Scer\GAL4^sca.PU has chaeta phenotype, suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has microchaeta phenotype, suppressible by Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU has macrochaeta phenotype, suppressible by Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-suppressible by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-suppressible by Dredd^B118/Dredd[+]

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-suppressible by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-suppressible by Dredd[+]/Dredd^L23

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has microchaeta phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Rala^S25N.cMa.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

Enhancer of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ras85D^V12.G37.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

NOT Enhancer of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Suppressor of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

NOT Suppressor of

Statement

Reference

Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Ras85D^V12.C40.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rgl^{1.Scer\UAS.T:Hsap\CAAX} suppresses the loss of bristle phenotype caused by expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU.

Co-expression of either Ras85D^{V12.C40.Scer\UAS} or Ras85D^{V12.G37.Scer\UAS} dramatically enhances the loss of bristle phenotype caused by expression of Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU.

The loss of bristle phenotype seen in flies co-expressing Ras85D^{V12.C40.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU is suppressed by co-expression of Rgl^{1.Scer\UAS.T:Hsap\CAAX}.

The loss of bristle phenotype seen in flies co-expressing Ras85D^{V12.G37.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU is largely suppressed by co-expression of Rgl^{1.Scer\UAS.T:Hsap\CAAX}.

Co-expression of R^{V12.cHa.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU results in a 10-13% survival rate. In all surviving flies, a wild-type pattern of macrochaeta and microchaeta bristles is restored on the thorax.

Co-expression of Rala^{S25N.cMa.Scer\UAS} has no effect on the rough eye phenotype caused by expression of Ras85D^{V12.C40.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

Co-expression of Rala^{S25N.cMa.Scer\UAS} enhances the rough eye phenotype caused by expression of Ras85D^{V12.G37.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

Co-expression of Rala^{S25N.cMa.Scer\UAS} partially suppresses the rough eye phenotype caused by expression of R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Mirey et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ral^S25N

(Balakireva et al., 2006)

Rala^{S25N.cMa.Scer\UAS}

Rala^S25N.cMa.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

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