FlyBase Allele Report: Dmel\Rap1[V12.cHa.UAS]

General Information

Symbol

Dmel\Rap1^V12.cHa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0221626

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

P{UAS-Rap1.V12.H}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Mirey et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Rap1.V12.H}

Cytology

Description

UAS regulatory sequences drive expression of an activated form of Rap1.

(Mirey et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

some die during embryonic stage, with Scer\GAL4^sca.PU

(Mirey et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Mirey et al., 2003)

hemocyte | embryonic stage

(Siekhaus et al., 2010)

Detailed Description

Statement

Reference

Haemocytes expressing R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^srp.Hemo form large, tightly associated masses of cells.

(Siekhaus et al., 2010)

Flies expressing R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^GMR.PF have a rough eye phenotype.

(Mirey et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Suppressed by

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU has lethal | embryonic stage phenotype, suppressible | partially by Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by Rgl^unspecified/Rgl^unspecified

(Mirey et al., 2003)

NOT suppressed by

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU has lethal | embryonic stage phenotype, non-suppressible by Rala^G20V.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU has lethal | embryonic stage phenotype, non-suppressible by Rgl^{1.UAS.Tag:PM(hKRAS)}, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Suppressor of

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU is a suppressor of visible phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Rgl^1FL.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Suppressed by

Statement

Reference

Rap1^V12.cHa.UAS has hemocyte | embryonic stage phenotype, suppressible by RhoL^10-161

(Siekhaus et al., 2010)

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Rala^S25N.cMa.UAS, Scer\GAL4^GMR.PF

(Mirey et al., 2003)

Rap1^V12.cHa.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Rgl^unspecified/Rgl^unspecified

(Mirey et al., 2003)

Suppressor of

Statement

Reference

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU is a suppressor of microchaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Rap1^V12.cHa.UAS, Scer\GAL4^sca.PU is a suppressor of macrochaeta phenotype of Rala^S25N.cMa.UAS, Scer\GAL4^sca.PU

(Mirey et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Hemocyte clustering observed in embryos expressing R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^srp.Hemo is abolished in the presence of RhoL^10-161.

(Siekhaus et al., 2010)

Co-expression of R^{V12.cHa.Scer\UAS} and Rala^{S25N.cMa.Scer\UAS} under the control of Scer\GAL4^sca.PU results in a 10-13% survival rate. In all surviving flies, a wild-type pattern of macrochaeta and microchaeta bristles is restored on the thorax.

Co-expression of Rala^{S25N.cMa.Scer\UAS} partially suppresses the rough eye phenotype caused by expression of R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

The rough eye phenotype caused by expression of R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^GMR.PF is partially suppressed in Rgl^unspecified homozygotes.

Co-expression of Rgl^1FL.Scer\UAS enhances the rough eye phenotype caused by expression of R^{V12.cHa.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Mirey et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR.PF/Rap1^V12.cHa.UAS rescues Rap1^S17A.UAS

(Dupuy et al., 2005)

Comments

Co-expression of R^{V12.cHa.Scer\UAS} rescues the lethality caused by expression of R^{S17A.Scer\UAS} under the control of Scer\GAL4^GMR.PF, and the rescued flies have normal sized eyes.

(Dupuy et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

R^{V12.cHa.Scer\UAS}

Rap1^{V12.cHa.Scer\UAS}

Rap1^V12.cHa.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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