Nucleotide substitution: C44683T.
The premature stop codon is in the L27N domain.
Amino acid replacement: Q?term.
C8235917T
C44683T
Q641term | sdt-PB; Q228term | sdt-PD; Q716term | sdt-PE; Q208term | sdt-PF; Q1369term | sdt-PG; Q283term | sdt-PH; Q618term | sdt-PI; Q857term | sdt-PJ; Q228term | sdt-PK; Q1271term | sdt-PL; Q1250term | sdt-PM; Q641term | sdt-PN; Q955term | sdt-PO; Q522term | sdt-PP
Q?term
Homozygous follicle cell clones show multilayering of cells in 31% of cases and show a flattened cells compared to wild-type cells in 37% of cases.
In sdtK85/+ retinas the photoreceptor cell stalk membrane is shorter than that of wild-type.
sdtK85 photoreceptor cell MARCM clones exhibit an approximate 50% reduction in stalk membrane length.
Photoreceptors in homozygous eye clones show severe defects, including a shortened stalk membrane, and they degenerate when exposed to light. This light-induced degeneration is strongly reduced if the animals are raised and maintained on vitamin A-depleted medium.
sdt[+]/sdtK85, w1118 has retina | conditional phenotype, suppressible by Scer\GAL4GMR.PF/sdtUAS.H.Tag:FLAG
sdt[+]/sdtK85 is an enhancer of retina | conditional phenotype of Scer\GAL4ninaE.PT, sdtUAS.D.Tag:FLAG, w1118
The light dependent retinal degeneration seen in w1118 flies does not depend on sdtK85/+. The rate of photoreceptor cell survival seen in a w1118 genetic background is similar in sdtK85/+ or +/+ flies.
Expression of sdtScer\UAS.H.T:Zzzz\FLAG in outer photoreceptor cells under the control of Scer\GAL4ninaE.PT in sdtK85/+ or +/+ genetic background completely blocked the light induced retinal degeneration in w1118 flies. In addition, the rhabdomeres of the outer Scer\GAL4ninaE.PT-expressing photoreceptor cells are reduced in size in comparison with control flies and flies including flies kept in the dark.
Expression of sdtScer\UAS.D.T:Zzzz\FLAG under the control of Scer\GAL4ninaE.PT enhances the retinal degeneration phenotype in w1118 eyes. When sdtScer\UAS.D.T:Zzzz\FLAG is expressed in a sdtK85/+ background, the decrease in photoreceptor cell survival is stronger.
sdtK85 is partially rescued by sdtA.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is partially rescued by sdtB2.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is not rescued by sdtΔN.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is not rescued by sdtΔL27C.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is not rescued by Scer\GAL4ninaE.PT/sdtΔPDZ.UAS.Tag:FLAG
sdtK85 is not rescued by sdtΔSH3.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is not rescued by sdtΔGUK.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
sdtK85 is not rescued by sdt1-L27C.UAS.Tag:FLAG/Scer\GAL4ninaE.PT
Expression of sdtA.Scer\UAS.T:Zzzz\FLAG or sdtB2.Scer\UAS.T:Zzzz\FLAG in sdtK85 photoreceptor cell MARCM clones partially restores talk-membrane length to a reduction of only 30% compared with wild-type. This stalk is significantly longer than that in photoreceptor cells mutant for sdtK85, but significantly shorter than stalk-membrane length in control photoreceptor cells.