FlyBase Allele Report: Dmel\sdt[XN]

General Information

Symbol

Dmel\sdt^XN

Species

D. melanogaster

Name

FlyBase ID

FBal0218370

Feature type

allele

Associated gene

Dmel\sdt

Associated Insertion(s)

Carried in Construct

Also Known As

sdt^XN05, sdt^N5

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Tepass and Knust, 1993)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. The mutation is within the 3' splice junction of exon 6.

(Hong et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,237,170..8,237,170 [+]

Nucleotide change:

G8237170A

Reported nucleotide change:

G?A

Comment:

A G to A mutation in the splice donor site.

(Hong et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Pagliarini and Xu, 2003)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Muller and Wieschaus, 1996, Perrimon et al., 1989)

some die during embryonic stage | recessive

(Muller and Wieschaus, 1996, Perrimon et al., 1989)

Phenotype Manifest In

embryonic epidermis

(Muller and Wieschaus, 1996)

eye photoreceptor cell stalk | somatic clone

(Berger et al., 2007)

zonula adherens

(Muller and Wieschaus, 1996)

Detailed Description

Statement

Reference

Homozygous eye clones have photoreceptors with normally shaped rhabdomeres. The stalk membrane is reduced by approximately 50% in these mutant photoreceptors. The mutant photoreceptors undergo retinal degeneration when exposed to light. This light-induced degeneration is strongly reduced if the animals are raised and maintained on vitamin A-depleted medium.

(Berger et al., 2007)

Homozygous embryos derived from germ line clones exhibit severe malformations or an absence of epidermis. sdt^XN baz^YD double mutants exhibit abnormal zonula adherens formation and mislocalisation of membrane protein neurotactin, phenotype is stronger than sdt^XP single mutant. Double mutants exhibit early cell shape changes, the surface of early gastrulating embryos shows irregularly shaped cell apices and in some areas may be multilayered by mid-gastrulation. Ultimately the cells undergo a complete transformation from epidermal to a mesenchymal-like morphology.

(Muller and Wieschaus, 1996)

No changes in phenotype of tor^13D embryos.

(Strecker et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sdt[+]/sdt^XN is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

sdt^XN/sdt^XN is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Pagliarini and Xu, 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

sdt[+]/sdt^XN is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Other

Statement

Reference

baz⁴, sdt^XN has embryonic/first instar larval cuticle phenotype

(Tanentzapf and Tepass, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Marked clones in the eye disc expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI and which are also homozygous for sdt^N5 show metastatic behaviour.

(Pagliarini and Xu, 2003)

Most sdt^XN baz⁴ double mutant embryos do not secrete any cuticle, while some embryos have a small number of cuticle grains that appear solid, rather than vesicular. The amount of cuticle produced in sdt^XN baz⁴ mutant embryos is not significantly increased if they also carry Df(3L)H99.

(Tanentzapf and Tepass, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Nrg^ibx

(Carhan et al., 2005)

Fails to complement

sdt^E195

(Berger et al., 2007)

sdt^K85