FlyBase Allele Report: Dmel\Drl-2[E124]

General Information

Symbol

Dmel\Drl-2^E124

Species

D. melanogaster

Name

FlyBase ID

FBal0217757

Feature type

allele

Associated gene

Dmel\Drl-2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Sakurai et al., 2009)

abnormal neuroanatomy (with Df(2R)Exel8057)

(Reynaud et al., 2015)

Phenotype Manifest In

adult mushroom body alpha-lobe (with Df(2R)Exel8057)

(Reynaud et al., 2015)

alpha/beta Kenyon cell (with Df(2R)Exel8057)

(Reynaud et al., 2015)

antennal lobe

(Sakurai et al., 2009)

antennal lobe glomerulus VL1

(Sakurai et al., 2009)

antennal lobe glomerulus VM7

(Sakurai et al., 2009)

glomerulus

(Sakurai et al., 2009)

presumptive embryonic salivary gland

(Harris and Beckendorf, 2007)

Detailed Description

Statement

Reference

Drl-2^E124/Df(2R)Exel8057 flies show defects in the mushroom body: approximately 60% lack the α lobe. In those animals where α axons are affected, 90% of the α axons show misguidance, while the rest show growth defects.

(Reynaud et al., 2015)

No lateral transverse muscle attachment defects are observed in Drl-2^E124 mutant stage 16 embryos.

(Lahaye et al., 2012)

Drl-2^E124 mutants display mild antennal lobe defects, but the commissure between the antennal lobes remains intact. Two glomeruli are observed in aberrant locations. VM7v is shifted dorsally slightly, whereas VL1 is positioned ventrally. The direction of movement of the glomeruli is clockwise.

(Sakurai et al., 2009)

Mutant embryos have salivary glands that curve ventrally at the tip, instead of lying parallel to the midline as in wild-type embryos.

(Harris and Beckendorf, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drl-2^E124 has abnormal neuroanatomy phenotype, enhanceable by drl^R343

(Sakurai et al., 2009)

Enhancer of

Statement

Reference

Drl-2^E124/Drl-2[+] is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^c739, Wnt5^UAS.cFa

(Reynaud et al., 2015)

drl[+], drl², Drl-2^E124, Drl-2[+] is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^c739, Wnt5^UAS.cFa

(Reynaud et al., 2015)

Drl-2^E124 is an enhancer of abnormal neuroanatomy phenotype of drl^R343

(Sakurai et al., 2009)

Suppressor of

Statement

Reference

Drl-2^E124 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^AM29, Wnt5^GS1192, drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^AM29, Wnt5^GS1192

(Sakurai et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drl-2^E124 has antennal lobe phenotype, enhanceable by drl^R343

(Sakurai et al., 2009)

Drl-2^E124 has glomerulus phenotype, enhanceable by drl^R343

(Sakurai et al., 2009)

Enhancer of

Statement

Reference

drl[+], drl², Drl-2^E124, Drl-2[+] is an enhancer of adult mushroom body alpha-lobe phenotype of Scer\GAL4^c739, Wnt5^UAS.cFa

(Reynaud et al., 2015)

Drl-2^E124/Drl-2[+] is an enhancer of adult mushroom body alpha-lobe phenotype of Scer\GAL4^c739, Wnt5^UAS.cFa

(Reynaud et al., 2015)

Drl-2^E124 is an enhancer of antennal lobe phenotype of drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is an enhancer of glomerulus phenotype of drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is an enhancer of olfactory receptor neuron phenotype of drl^R343

(Sakurai et al., 2009)

NOT Enhancer of

Statement

Reference

Drl-2^E124 is a non-enhancer of lateral transverse muscle cell | embryonic stage 16 phenotype of drl^Red2

(Lahaye et al., 2012)

Suppressor of

Statement

Reference

Drl-2^E124 is a suppressor of glomerulus phenotype of Scer\GAL4^AM29, Wnt5^GS1192, drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is a suppressor of antennal lobe phenotype of Scer\GAL4^AM29, Wnt5^GS1192, drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is a suppressor of olfactory receptor neuron phenotype of Scer\GAL4^AM29, Wnt5^GS1192, drl^R343

(Sakurai et al., 2009)

Drl-2^E124 is a suppressor of glomerulus phenotype of Scer\GAL4^AM29, Wnt5^GS1192

(Sakurai et al., 2009)

Drl-2^E124 is a suppressor of antennal lobe phenotype of Scer\GAL4^AM29, Wnt5^GS1192

(Sakurai et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the mushroom body α axon misguidance phenotype seen in flies expressing two copies of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^c739 is significantly enhanced (to over 10%) if they are also carrying either drl²/+ or Drl-2^E124/+. Flies simultaneously carrying both drl²/+ and Drl-2^E124/+ and expressing two copies of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^c739 show misguidance of α axons in over 40% of cases.

(Reynaud et al., 2015)

Drl-2^E124 does not enhance the lateral transverse muscle attachment defects seen in drl^Red2 mutant stage 16 embryos.

(Lahaye et al., 2012)

Double mutant drl^R343 Drl-2^E124 flies frequently lack the commissure between antennal lobes and often exhibit repositioning of glomeruli in a clockwise manner.

Overexpression of Wnt5^GS1192 in drl^R343 mutant olfactory receptor neurons (under the control of Scer\GAL4^AM29) severely affects axonal projections; olfactory receptor neuron axons target ectopic regions near the original antennal lobe positions, enhancing the drl^R343 mutant phenotype. This enhancement is ameliorated through the loss of Drl-2 in Drl-2^E124 mutants. Olfactory receptor neuron axons project to the normal antennal lobe positions in these triple mutants. The glomerular pattern is similar to that of drl^R343 Drl-2^E124 double mutants.

(Sakurai et al., 2009)

Drl-2^E124 ; drl^R343 double heterozygotes show salivary gland guidance defects; the salivary glands curve ventromedially towards the central nervous system, instead of lying parallel to the midline as in wild-type embryos.

(Harris and Beckendorf, 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Df(2R)Exel8057/Drl-2^E124 is partially rescued by Drl-2^UAS.Tag:MYC/Scer\GAL4^c739

(Reynaud et al., 2015)

Df(2R)Exel8057/Drl-2^E124 is partially rescued by Scer\GAL4^elav-C155/Drl-2^UAS.Tag:MYC

(Reynaud et al., 2015)

Not rescued by

Df(2R)Exel8057/Drl-2^E124 is not rescued by Scer\GAL80^Mef2.mb247/Drl-2^UAS.Tag:MYC/Scer\GAL4^c739

(Reynaud et al., 2015)

Df(2R)Exel8057/Drl-2^E124 is not rescued by Scer\GAL4^elav-C155/Scer\GAL80^Mef2.mb247/Drl-2^UAS.Tag:MYC

(Reynaud et al., 2015)

Df(2R)Exel8057/Drl-2^E124 is not rescued by Scer\GAL4^Tab2-201Y/Drl-2^UAS.Tag:MYC

(Reynaud et al., 2015)

Df(2R)Exel8057/Drl-2^E124 is not rescued by Scer\GAL4^c305a/Drl-2^UAS.Tag:MYC

(Reynaud et al., 2015)

Df(2R)Exel8057/Drl-2^E124 is not rescued by Drl-2^{Δcyto.UAS.Tag:MYC}/Scer\GAL4^c739

(Reynaud et al., 2015)

Comments

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 rescues the formation of the mushroom body α lobe in the majority of Drl-2^E124/Df(2R)Exel8057 flies, with the degree of rescue depending on the number of copies of Drl-2^{Scer\UAS.T:Hsap\MYC} used. This rescue is suppressed by the co-expression of Scer\GAL80^Mef2.mb247.

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 significantly rescues the formation of the mushroom body α lobe in Drl-2^E124/Df(2R)Exel8057 flies, but this rescue is suppressed by the co-expression of Scer\GAL80^Mef2.mb247.

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^Tab2-201Y or Scer\GAL4^c305a fails to rescue the formation of the mushroom body α lobe in Drl-2^E124/Df(2R)Exel8057 flies.

Expression of Drl-2^{Δcyto.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 fails to rescue the formation of the mushroom body α lobe in Drl-2^E124/Df(2R)Exel8057 flies.

(Reynaud et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64302

y¹ w^67c23; Drl-2^E124/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Drl-2^E124

(Reynaud et al., 2015, Lahaye et al., 2012, Sakurai et al., 2009, Harris and Beckendorf, 2007)

drl2^E124

(Zhu et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (6)

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