FlyBase Allele Report: Dmel\Drl-2[UAS.Tag:MYC]

General Information

Symbol

Dmel\Drl-2^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0317400

Feature type

allele

Associated gene

Dmel\Drl-2

Associated Insertion(s)

Carried in Construct

PBac{UAS-Drl-2.WT.MYC}

Key Links

Transgenic product class

wild_type

(Reynaud et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Reynaud et al., 2015)

Mutagen

in vitro construct

(Reynaud et al., 2015)

Progenitor genotype

Carried in construct

PBac{UAS-Drl-2.WT.MYC}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Drl-2 tagged with Tag:MYC.

(Reynaud et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Drl-2

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^eg-Mz360

(Reynaud et al., 2015)

Phenotype Manifest In

larval posterior commissure | embryonic stage, with Scer\GAL4^eg-Mz360

(Reynaud et al., 2015)

Detailed Description

Statement

Reference

Expression of two copies of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^eg-Mz360 in embryos results in more than 95% of axon commissures showing a switching phenotype where the posterior commissure axons cross to the adjacent anterior commissure.

(Reynaud et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drl-2^UAS.Tag:MYC, Scer\GAL4^eg-Mz360 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Wnt5⁴⁰⁰

(Reynaud et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155, Scer\GAL80^Mef2.mb247, Drl-2^UAS.Tag:MYC is a suppressor | partially of abnormal neuroanatomy phenotype of drl²/drl^R343

(Reynaud et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drl-2^UAS.Tag:MYC, Scer\GAL4^eg-Mz360 has larval posterior commissure | embryonic stage phenotype, suppressible by Wnt5⁴⁰⁰

(Reynaud et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155, Scer\GAL80^Mef2.mb247, Drl-2^UAS.Tag:MYC is a suppressor | partially of adult mushroom body alpha-lobe phenotype of drl²/drl^R343

(Reynaud et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The axon commissure switching phenotype seen in embryos expressing two copies of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^eg-Mz360 is completely suppressed by Wnt5⁴⁰⁰/Y.

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control Scer\GAL4^elav-C155 in the presence of Scer\GAL80^Mef2.mb247 partially rescues the formation of the mushroom body α lobe in drl²/drl^R343 flies.

(Reynaud et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Drl-2^UAS.Tag:MYC/Scer\GAL4^c739 partially rescues Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Scer\GAL4^elav-C155/Drl-2^UAS.Tag:MYC partially rescues Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Fails to rescue

Scer\GAL80^Mef2.mb247, Drl-2^UAS.Tag:MYC, Scer\GAL4^c739 fails to rescue Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Scer\GAL4^elav-C155, Scer\GAL80^Mef2.mb247, Drl-2^UAS.Tag:MYC fails to rescue Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Scer\GAL4^Tab2-201Y/Drl-2^UAS.Tag:MYC fails to rescue Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Scer\GAL4^c305a/Drl-2^UAS.Tag:MYC fails to rescue Df(2R)Exel8057/Drl-2^E124

(Reynaud et al., 2015)

Comments

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c739 rescues the formation of the mushroom body α lobe in the majority of Drl-2^E124/Df(2R)Exel8057 flies, with the degree of rescue depending on the number of copies of Drl-2^{Scer\UAS.T:Hsap\MYC} used. This rescue is suppressed by the co-expression of Scer\GAL80^Mef2.mb247.

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 significantly rescues the formation of the mushroom body α lobe in Drl-2^E124/Df(2R)Exel8057 flies, but this rescue is suppressed by the co-expression of Scer\GAL80^Mef2.mb247.

Expression of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^Tab2-201Y or Scer\GAL4^c305a fails to rescue the formation of the mushroom body α lobe in Drl-2^E124/Df(2R)Exel8057 flies.

(Reynaud et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64341

w¹¹¹⁸; PBac{UAS-Drl-2.WT.MYC}VK00027

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Drl-2^{Scer\UAS.T:Hsap\MYC}

Drl-2^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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