FlyBase Allele Report: Dmel\dl[UASp.cMa]

General Information

Symbol

Dmel\dl^UASp.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0212140

Feature type

allele

Associated gene

Dmel\dl

Associated Insertion(s)

Carried in Construct

P{UASp-dl.M}

Also Known As

UASp-dorsal

Key Links

Transgenic product class

cDNA

(Matova and Anderson, 2006)

wild_type

(Matova and Anderson, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Matova and Anderson, 2006)

wild_type

(Matova and Anderson, 2006)

Mutagen

in vitro construct

(Matova and Anderson, 2006)

Progenitor genotype

Carried in construct

P{UASp-dl.M}

Cytology

Description

UASp regulatory sequences drive expression of the dl-A isoform.

(Minidorff et al., 2007)

UASp regulatory sequences drive expression of dl cDNA.

(Matova and Anderson, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR.PF

(Minidorff et al., 2007)

lethal, with Scer\GAL4^hs.PB

(Matova and Anderson, 2006)

Phenotype Manifest In

axon & eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Minidorff et al., 2007)

lamina, with Scer\GAL4^GMR.PF

(Minidorff et al., 2007)

medulla, with Scer\GAL4^GMR.PF

(Minidorff et al., 2007)

Detailed Description

Statement

Reference

When dl^Scer\UAS.cMa is expressed under the control of Scer\GAL4^GMR.PF, some photoreceptor R2-R5 axons project past the lamina and into the medulla.

(Minidorff et al., 2007)

Expression of dl^Scer\UAS.cMa, driven by Scer\GAL4^hs.PB at 37^oC (as a 1 hour pulse) is lethal.

(Matova and Anderson, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

dl^UASp.cMa, dl¹, Scer\GAL4^hs.PB is a suppressor of lethal | larval stage phenotype of Dif¹

(Matova and Anderson, 2006)

Scer\GAL4^e33C, dl^UASp.cMa, dl¹ is a suppressor of lethal | larval stage phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of lethal | larval stage phenotype of Dif¹

(Matova and Anderson, 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^e33C, dl^UASp.cMa, dl¹ is a suppressor of hemocyte phenotype of Dif¹

(Matova and Anderson, 2006)

Scer\GAL4^e33C, dl^UASp.cMa, dl¹ is a suppressor of hemolymph phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of hemocyte phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^He.PZ, dl¹ is a suppressor of hemolymph phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemocyte phenotype of Dif¹

(Matova and Anderson, 2006)

dl^UASp.cMa, Scer\GAL4^srp.Hemo, dl¹ is a suppressor of hemolymph phenotype of Dif¹

(Matova and Anderson, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Ubiquitous expression of dl^Scer\UAS.cMa, driven by Scer\GAL4^hs.PB at 25^oC (without heat shock) fully rescues the lethality of Dif¹ dl¹ double mutants.

Expression of dl^Scer\UAS.cMa in circulating haemocytes, lymph glands, and epidermis, but not in the fat body, under the control of Scer\GAL4^e33C, restores normal haemocyte numbers in Dif¹ dl¹ double mutants. The rescued blood cells are indistinguishable in morphology from wild-type uninfected haemocytes. In addition, the rescued animals have do not have any microbes in their haemolymph and 66% survive to adult stages.

Expression of dl^Scer\UAS.cMa in circulating haemocytes, but not in other immune-responsive tissues, under the control of Scer\GAL4^He.PZ, results in an absence of microbes from the haemolymph, with approximately 45% of dl^Scer\UAS.cMa expressing Dif¹ dl¹ double mutants surviving till adulthood.

Expression of dl^Scer\UAS.cMa in circulating haemocytes and lymph gland cells, under the control of Scer\GAL4^srp.Hemo, increases blood cell number to approximately 90% of wild-type in Dif¹ dl¹ double mutants and enables approximately 50% survival till adulthood. The majority of these larvae have no microbes in their haemolymph.

(Matova and Anderson, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dl^Scer\UAS.cMa

dl^UASp.cMa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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