FlyBase Allele Report: Dmel\crb[GD14463]

General Information

Symbol

Dmel\crb^GD14463

Species

D. melanogaster

Name

FlyBase ID

FBal0209289

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

Carried in Construct

P{GD14463}

Also Known As

UAS-crb RNAi, crb RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14463}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14463

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Steffensmeier et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^en-e16E

(Richardson and Pichaud, 2010)

abnormal size, with Scer\GAL4^ey.PH

(Richardson and Pichaud, 2010)

abnormal size | adult stage, with Scer\GAL4^nub-AC-62

(Su et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PH

(Richardson and Pichaud, 2010)

embryonic/larval tracheal system | embryonic stage 17, with Scer\GAL4^btl.PS

(Stümpges and Behr, 2011)

eye, with Scer\GAL4^ey.PH

(Richardson and Pichaud, 2010)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^en-e16E

(Richardson and Pichaud, 2010)

wing blade, with Scer\GAL4^nub-AC-62

(Su et al., 2017)

Detailed Description

Statement

Reference

Expression of crb^GD14463 under the control of Scer\GAL4^nub-AC-62 results in a moderate wing overgrowth in adult flies: the wing area is significantly increased compared to controls.

(Su et al., 2017)

Expression of crb^GD14463 under the control of Scer\GAL4^btl.PS at 25[o]C does not alter the morphology of transition zone tubes.

(Song et al., 2013)

Expression of crb^GD14463 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^repo.PU has no detectable effect on glial cell number in the third instar larval eye disc.

(Reddy and Irvine, 2011)

Stage 17 embryos expressing crb^GD14463 under the control of Scer\GAL4^btl.PS show severe defects in the transition from luminal liquid-clearance to air-filled airways.

(Stümpges and Behr, 2011)

Adults expressing crb^GD14463 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of crb^GD14463 under the control of Scer\GAL4^ey.PH leads to an increase in head and eye size compared with controls.

Expression of crb^GD14463 under the control of Scer\GAL4^en-e16E leads to the overgrowth of the posterior compartment of the wing.

(Richardson and Pichaud, 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14463

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, crb^GD14463 has abnormal size | adult stage phenotype, suppressible by ex^UASp.EGFP/Scer\GAL4^nub-AC-62

(Su et al., 2017)

Scer\GAL4^nub-AC-62, crb^GD14463 has abnormal size | adult stage phenotype, suppressible by Scer\GAL4^nub-AC-62/kibra^UASp.cYa

(Su et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^nub-AC-62/crb^GD14463 is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^nub-AC-62, kibra^UASp.cYa

(Su et al., 2017)

Suppressor of

Statement

Reference

crb^GD14463, Scer\GAL4^GMR.PU is a suppressor of abnormal neuroanatomy phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Steffensmeier et al., 2013)

crb^GD14463, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Steffensmeier et al., 2013)

crb^GD14463, Scer\GAL4^GMR.PU is a suppressor of increased cell death phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Steffensmeier et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, crb^GD14463 has wing blade phenotype, suppressible by ex^UASp.EGFP/Scer\GAL4^nub-AC-62

(Su et al., 2017)

Scer\GAL4^nub-AC-62, crb^GD14463 has wing blade phenotype, suppressible by Scer\GAL4^nub-AC-62/kibra^UASp.cYa

(Su et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^nub-AC-62/crb^GD14463 is an enhancer of wing blade phenotype of Scer\GAL4^nub-AC-62, kibra^UASp.cYa

(Su et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^btl.PS/crb^GD14463 is a suppressor of terminal tracheal cell | third instar larval stage phenotype of GckIII^wheezy-770

(Song et al., 2013)

crb^GD14463, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Steffensmeier et al., 2013)

crb^GD14463, Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Steffensmeier et al., 2013)

NOT Suppressor of

Statement

Reference

crb^GD14463/Scer\GAL4^GR1 is a non-suppressor of follicle cell | somatic clone phenotype of Tao^eta

(Gomez et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The wing undergrowth observed in flies expressing either ex^{Scer\UAS.P\T.T:Avic\GFP-EGFP} or kibra^{Scer\UAS.P\T.cYa} under the control of Scer\GAL4^nub-AC-62 (their wing area is significantly decreased compared to controls) is modified by co-expression of crb^GD14463 (which on its own leads to wing overgrowth), but whereas the small wing size of ex^{Scer\UAS.P\T.T:Avic\GFP-EGFP}-expressing flies is increased, in the case of kibra^{Scer\UAS.P\T.cYa}, the wing size is further reduced by co-expression of crb^GD14463.

(Su et al., 2017)

Expression of crb^GD14463 under the control of Scer\GAL4^btl.PS partially suppresses the tracheal morphogenesis defects seen in GckIII^wheezy-770 mutant third instar larvae. Transition zone dilation is completely suppressed in 61% of terminal cells and partially suppressed in a further 27%.

(Song et al., 2013)

The failure of anterior follicle cells in homozygous Tao^eta stage 9 follicle cell clones to stretch is not suppressed if the clones are also expressing crb^GD14463 under the control of Scer\GAL4^GR1.

(Gomez et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of crb^GD14463 significantly rescues the eye neurodegeneration phenotype caused by expression of Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU. The increased cell death phenotype which is seen in the eye imaginal discs of animals expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU is also significantly suppressed.

(Steffensmeier et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v39177

w¹¹¹⁸; P{GD14463}v39177

Notes on Origin

Discoverer